executive summary - National Dairy Research Institute

Research Achievements
milk production/productivity was found maximum
with the increase of pregnancy rate in first parity and
minimum in fourth parity of Murrah buffaloes.
The center has produced 60,355 doses of frozen semen
of which 15,410 doses of frozen semen from five test
bulls and has supplied 900 doses of frozen semen to
other centers. In addition 3882 doses of semen were
supplied to NDRI field unit and 22,361 doses of frozen
semen to farmers and other dairy development
organizations during the period. The germplasm of
genetically superior progeny tested proven bulls has
been used on elite cows in organized herds for
production of high-pedigreed bulls for further
multiplication and production of superior germplasm
and establishment of elite herds. Superior semen of
proven and high-pedigreed bulls of the Center has also
been used by various dairy development agencies and
dairy farmers for bringing genetic improvement of
Murrah buffaloes.
Network Project on Buffalo Improvement (Field
Unit)
A total of 2792 AI were performed in Murrah buffaloes
under field conditions and as a result 44.53 conception
rate was obtained. The highest conception rate was
observed in the month of March (68%) and the lowest
was in the month of September (44.62%). Across the
villages, the highest conception rate was observed in
Rindal (54.08%) and lowest was observed in the village
Shekhpura (38.15%). A total of 1120 (573 male and 547
female) Murrah buffalo calves were born in the
farmers' herds and performance data on 138 daughters
have been recorded for evaluation of bulls under field
conditions. As many as 15 breeding bulls belonging to
th
the 12 set were used for AI during the year.
A daughter from Murrah bull born under field conditions
Fluorescent in situ hybridization (FISH) and
Associated Techniques for Characterization of
Chromosomes for Detection of Genetical and
Reproductive Problems in Cattle, Buffalo and
Goats
Investigations were carried out for identification and
inheritance pattern of fecundity genes in cattle, with
comparison of DNA fragment between dams bearing
twin and singleton. The study involved analysis of
genome related to twinning using molecular markers
(PCR-RFLP and SSCP) on BMPR-1B, BMP-15 and
GDF-9 genes pure (Sahiwal and Hariana) and three
crossbreeds (Karan Swiss, Karan Fries, Holstein
Friesian) besides other cross breeds of unknown
lineage selected from field. Molecular study was
carried out using 18 primers on the DNA of 51 animals
out of which 20 were experimental dams, 6 were
control dams and 25 were twin progenies. PCR-RFLP
analysis of Exon 6 of the BMPR-1B gene using AvaII
revealed monomorphic band pattern of the wild-type
allele in the sampled animals. Two of the BMP-15
mutation alleles FecXG and FecXI investigated using
HinfI and XbaI revealed monomorphic pattern of the
wild type allele for dams and their twin progenies.
Exon 1 of the GDF-9 gene amplified to produce 462bp
fragment and was subjected to HhaI and HinfI
restriction enzyme which revealed homozygous
pattern of the mutant allele. PCR-SSCP analysis of the
BMPR-1B gene detected monomorphic pattern for this
gene mutation. The progenies inherited similar
monomorphic band from their dams for this gene locus.
In BMP-15 locus polymorphic band pattern were
detected for one region, while two other regions
showed monomorphic pattern for this gene locus.
SSCP pattern analysis for bovine Bmp-15 gene
revealed monomorphism for two regions in exon 1 and
exon 2, while one region in exon 2 revealed
polymorphic pattern in both experimental and control
dams. PCR analysis of the FecXR allele of the BMP-15
gene detected the presence of wild-type allele in the
dams and their progenies. An allele specific primer
used for detection of GDF-9 gene mutation, identified
only wild-type and control fragment which revealed
the absence of the mutant fragment for this gene locus.
The study on the three fecundity genes BMPR-1B,
BMP-15 and GDF- 9 did not show association with
twinning trait in cattle.
ANIMAL BIOTECHNOLOGY
Faster Multiplication of Superior Germplasm for
Development of National Milch Herds 'Garima-II'
a Cloned Buffalo Gives Birth to a Normal Calf
'Mahima', a female calf weighing 32 kg was born on
January 25 to 'Garima-II' a cloned buffalo, which had
been produced by Hand-guided cloning using
Annual Report - 2012-13
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