executive summary - National Dairy Research Institute

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<strong>Research</strong> Achievements Epithelial Cells (MEC) at different stages of lactation metabolism. PCA was used to reduce the by differential gel electrophoresis (DIGE) followed by dimensionality of a multidimensional analysis to identification of the proteins by mass spectrometry. display the two principle components that distinguish MECs were isolated from milk of 4 Sawihal cows from between the two large sources of variation within the each stage which constituted early, peak and late (30, dataset. For the 44 features identified in the ~70 and 250 days in lactation, respectively) using experiment, the first principle component immunoaffinity-based methods. Protein samples were distinguished 54.9 % of the variance with 40.6 % prepared from isolated MECs and subjected to additional variation distinguished by the second isoelectric focusing in pH 4-7 gels. A total of 1523 principle component. Categorization of differentially resolved protein features, for different stages of expressed proteins for early, peak and late stages lactation, were matched across 6 gels. These included showed that more than one-third of identified bovine many isoforms that resulted from charged post- MEC proteins account for binding activity, which is translational modification and/or processing. A essential for cell-cell interaction, cell-matrix comparison of bovine MEC at different stages of interaction which plays role in growth, development lactation allowed identification of 44 proteins whose and remodeling of mammary gland throughout the expression varied with the stage of lactation. The most stages of lactation and rest are involved in structural strongly regulated proteins were cytoskeletal molecule activity, catalytical activity, enzyme components, regulators of protein folding and stability, regulator activity, receptor activity and motor activity. calcium-binding proteins, and regulators of cellular DIGE analysis of early vs peak vs late samples. DIGE images were scanned after SDS-PAGE. The gels were scanned using all the three lasers corresponding to Cy2, Cy3 and Cy5 wave lengths. The images were taken at 200 µ resolution. The green colour are down regulating and red colour spots represent up regulation. Categorization of differentially expressed proteins at different stages of lactation based on the molecular function Expression Analysis of Insulin-Like Growth Factor Binding Proteins (IGFBPs) in Milk of Murrah Buffaloes and Sahiwal Cows During Lactation Expression profiling of different IGFBPs was carried out during lactation in Sahiwal cows and Murrah buffaloes to understand their role in lactation persistency and explore their source in milk. Milk 15 Annual Report - 2012-13
<strong>Research</strong> Achievements Comparision between colostrum, peak and involution stages of lactation in Murrah buffaloes and Sahiwal cows Figure:Expression and purification of PepX:SDS-PAGE shows the purified PepX protein in Unbound fraction (Lane 1), Partially purified PepX using Ni-NTA affinity chormatography (Lane 2), and PepX purified by MonoQ anion exchange column (Lane 3). Annual Report - 2012-13 16
Page 3 and 4: CONTENTS Milestones, Goals & Mandat
Page 5 and 6: izkDdFku PREFACE jk"Vªh; Msjh vuql
Page 7 and 8: Preface izlaLd`r pht+ LizsM+] HksSa
Page 9 and 10: EXECUTIVE SUMMARY National Dairy Re
Page 11 and 12: Executive Summary • Area specific
Page 13 and 14: Executive Summary • The Institute
Page 15 and 16: Executive Summary Annual Report - 2
Page 17 and 18: ORGANISATIONAL SETUP Introduction T
Page 19 and 20: Introduction Dr. B. S. Bisht, Vice-
Page 21 and 22: 2. RESEARCH ACHIEVEMENTS DAIRY CATT
Page 23 and 24: Research Achievements PCR-RFLP of c
Page 25 and 26: Research Achievements milk producti
Page 27 and 28: Research Achievements the expressio
Page 29: Research Achievements CYTOCHROME C,
Page 33 and 34: Comparative pattern of TSPY gene do
Page 35 and 36: Research Achievements groups. In mi
Page 37 and 38: Research Achievements Neuro-Fuzzy s
Page 39 and 40: Research Achievements Testicular ce
Page 41 and 42: Research Achievements reproductive
Page 43 and 44: Research Achievements Modulation of
Page 45 and 46: Research Achievements Effect of Sel
Page 47 and 48: Research Achievements Overall milk
Page 49 and 50: Research Achievements Annual Report
Page 51 and 52: Research Achievements Annual Report
Page 53 and 54: Research Achievements (A) MSCs diff
Page 55 and 56: Research Achievements Particle size
Page 57 and 58: Research Achievements through this
Page 59 and 60: Research Achievements IPCS, PPCS, O
Page 61 and 62: Research Achievements protein in co
Page 63 and 64: Research Achievements Investigation
Page 65 and 66: Research Achievements Research on N
Page 67 and 68: Research Achievements Lactobacillus
Page 69 and 70: Research Achievements 1 H-NMR Spect
Page 71 and 72: Research Achievements Production of
Page 73 and 74: Research Achievements Miniaturized
Page 75 and 76: Research Achievements 2 kg/cm ). Op
Page 77 and 78: Research Achievements Selected Whol
Page 79 and 80: Research Achievements 485±32 and 5
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Research Achievements from one of t
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Research Achievements % NPN, 6 to 1
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Research Achievements gulabjamun wi
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Research Achievements of frying wer
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Research Achievements The training
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Research Achievements in different
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Sr. No DAIRY EXTENSION DIVISION Fie
Page 95 and 96:
Transfer of Technology Sponsored Co
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Transfer of Technology AGRICULTURE
Page 99 and 100:
Dairy Education Counseling for Admi
Page 101 and 102:
5. HONOURS/AWARDS • A project ent
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Honours/Awards Processed Cheese Spr
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Honours/Awards Mahesh V. Chaudhary
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6. RESEARCH COLLABORATIONS The scie
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Research Collaboration 31. Developm
Page 111 and 112:
7. EMPOWERMENT OF WOMEN & MAINSTREA
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INSTITUTE PUBLICATIONS Aggarwal, An
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Publications and growth curve in Sa
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Publications Kumaresan, A. Johannis
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Publications Salinomycin addition i
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Publications Upadhyay, R. C., Singh
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Publications Dutta, R. (2011) Isola
Page 125 and 126:
Publications antiobesity potential.
Page 127 and 128:
Publications Book Chapter clarifica
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Publications 128-129. Hati, S., Vij
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Publications (2012) Soy based ferme
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Publications Singh, S.K. (2012) Res
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Publications Gupta, Jancy (2012) Da
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Publications Livestock Sci.. 152 :
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Consultancy, Patents and Commercial
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PME the Institute were also documen
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Human Resource Development Dr. S. K
Page 145 and 146:
Human Resource Development Dr. Chan
Page 147 and 148:
Human Resource Development Dr. S. K
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Human Resource Development Dr. Biml
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Human Resource Development Dr. R. K
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13. DISTINGUISHED VISITORS 12.04.20
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14. PERSONNEL INSTITUTE STAFF st As
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Personnel A. K. Chauhan, Ph.D. Prin
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Personnel Veeraju, B.E. (Civil) Tec
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Personnel was promoted to T-9 w.e.f
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15. MAIN STATION RESEARCH DIVISIONS
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Research Division processes for pro
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Research Division methods of manufa
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Support Sections st nd nd rd • Au
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Support Sections Month-wise milking
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Support Sections Reproductive Statu
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Support Sections Production and Pro
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Support Sections Sale of the Dairy
Page 180 and 181:
Support Sections Exhibitions Organi
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16. REGIONAL STATIONS SOUTHERN REGI
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Regional Stations • Training prog
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Regional Stations also converted to
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Regional Stations Production of dif
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Regional Stations • Total 433 liv
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executive summary - National Dairy Research Institute

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