executive summary - National Dairy Research Institute

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<strong>Research</strong> Achievements • Artificial Neural Network (ANN) algorithm standard protocols. There was no significant difference (maximum accuracy) found best suited for observed in scrotal circumference and testicular length detection of lameness for routine screening of before and after the treatment. The testicular width was cows using “wireless sensor controlled four 6.59±0.32 and 7.08±0.27 during pre and post quadrant load cell platform”, based on percent aspiration, while the corresponding values for body weight distribution on individual legs of testicular mass were 355.77±39.20 and 427±28.75, Karan Fries cattle. respectively. There was no significant changes in ejaculate volume (4.63±0.33 Vs 4.73±0.20), mass • Appropriate Neuro-fuzzifiers for mastitis activity (2.32±0.33 Vs 2.42±0.22) and individual classification was developed. Fuzzy logic motility (53.15±4.29 Vs 58.60 ± 3.19) in bulls during algorithm once developed and standardized for pre- PNAB and post- PNAB period. Similarly, the a particular farming situation can be efficiently percentage of live spermatozoa, membrane intact used for automated subclinical mastitis spermatozoa and acrosome intact spermatozoa did not detection (Figure 1). Artificial neural network differ significantly between pre- PNAB and postalgorithm showed a good accuracy rate for PNAB period. These results indicated that detecting clinical mastitis from that of healthy percutaneous needle aspiration biopsy technique can animals. be used as a diagnostic method to detect sub-fertility • A DSS module for reproduction and production and infertility in crossbred bulls without affecting their management of crossbred and Murrah buffaloes reproductive health. to improve accuracy and efficiency of heat Testicular cell indices and peripheral blood detection, milking production and heifer growth testosterone concentrations in relation to age and was developed. semen quality in crossbred (KF) bulls: The changes Infertility in Crossbred Bulls: Search for in peripheral blood testosterone concentration and Spermatogenic Cell Markers for Early Prediction testicular cytogram in relation to age and semen quality of Fertility (NFBSFARA– 3009) were studied in Karan Fries crossbred males. Three different age groups of crossbred males viz. bull calves Percutaneous needle aspiration biopsy method for (6 months, n = 5), young bulls (15 months, n = 5) and extraction of testicular cells from live animals: A adult bulls (4-6 years, n = 8) were utilized for the study. method for extraction of testicular cells from bulls, Percutaneous needle aspiration biopsy technique was percutaneous needle aspiration biopsy (PNAB), was used to quantify testicular cytology and their indices. standardized. The needle length, gauge and site of Peripheral blood testosterone concentrations were aspiration were standardized in morbid specimen and measured by ELISA method. Semen collected from then in live goats, calves and then the standardized adult bulls was evaluated for quality parameters. Mean technique were applied on adult breeding bulls. The peripheral blood testosterone concentrations in bull animals were restrained properly and epidural calves, young bulls and adult bulls were 2.28 ± 0.09 anesthesia was administered at sacro-coccygeal ng/ml, 1.42 ± 0.22 ng/ml and 5.66 ± 1.08 ng/ml junction. By this procedure, desensitization of the respectively, and the difference was significant (P < posterior part of animals was achieved within 3-5 0.01). There was no significant difference (P > 0.05) minutes. The testes were held tightly with hand and between the proportion of different testicular cells in antiseptic solution was applied over the scrotum. A 22 bull calves and young bulls. Between young and adult gauge needle attached to 5 mL syringe was inserted bulls, significant difference (P < 0.01) was observed in into the testis through scrotum at right angle to the the proportion of spermatocytes, spermatozoa, and testis. When plunger was pulled back, the needle was sperm: Sertoli cell ratio. The proportions of Sertoli moved little forward and backward within the testis cells showed significant difference (P < 0.01) between two to three times for approximately 4 seconds for the three groups. The number of primary spermatocytes dislodging of cells and easy suction into the needle. It had positive correlation with peripheral blood was found that the aspirates, obtained using the testosterone concentrations in bull calves (r = 0.719, P standardized method, were enough to study the < 0.01). Sertoli cells per 100 germ cells were negatively testicular cytogram. correlated with testosterone concentration in young Effect of PNAB procedure on semen quality and testicular morphology: A preliminary study was bulls (r = -0.713, P < 0.01). Among different semen parameters in adult bulls, ejaculate volume (r = 0.790, P conducted to assess the effect of PNAB technique on = 0.01) had positive relationship, and sperm motility testicular biometry and seminal characteristics in had significant negative correlation (r = -0.711, P = Karan Fries crossbred bulls. Eight bulls within the age 0.048) with testosterone concentrations. These results range of 4-6 years were utilized for study. Before and indicated that quantification of testicular cells and after fine needle aspiration, testicular parameters were indices could be used to assess the semen production measured and seminal quality was assessed as per ability of bulls. 23 Annual Report - 2012-13
<strong>Research</strong> Achievements Testicular cells at 40 X (Left) and at 100 X (Right) magnifications Sertoli cell Spermatogonia Spermatocyte Early spermatids Late spermatids Spermatozoa Testicular cytology of crossbred bulls using the standardized percutaneous needle aspiration biopsy technique Peri-post-partum follow up protocol for reducing uterine infection in dairy cattle and buffaloes Annual Report - 2012-13 24
Page 3 and 4: CONTENTS Milestones, Goals & Mandat
Page 5 and 6: izkDdFku PREFACE jk"Vªh; Msjh vuql
Page 7 and 8: Preface izlaLd`r pht+ LizsM+] HksSa
Page 9 and 10: EXECUTIVE SUMMARY National Dairy Re
Page 11 and 12: Executive Summary • Area specific
Page 13 and 14: Executive Summary • The Institute
Page 15 and 16: Executive Summary Annual Report - 2
Page 17 and 18: ORGANISATIONAL SETUP Introduction T
Page 19 and 20: Introduction Dr. B. S. Bisht, Vice-
Page 21 and 22: 2. RESEARCH ACHIEVEMENTS DAIRY CATT
Page 23 and 24: Research Achievements PCR-RFLP of c
Page 25 and 26: Research Achievements milk producti
Page 27 and 28: Research Achievements the expressio
Page 29 and 30: Research Achievements CYTOCHROME C,
Page 31 and 32: Research Achievements Comparision b
Page 33 and 34: Comparative pattern of TSPY gene do
Page 35 and 36: Research Achievements groups. In mi
Page 37: Research Achievements Neuro-Fuzzy s
Page 41 and 42: Research Achievements reproductive
Page 43 and 44: Research Achievements Modulation of
Page 45 and 46: Research Achievements Effect of Sel
Page 47 and 48: Research Achievements Overall milk
Page 49 and 50: Research Achievements Annual Report
Page 51 and 52: Research Achievements Annual Report
Page 53 and 54: Research Achievements (A) MSCs diff
Page 55 and 56: Research Achievements Particle size
Page 57 and 58: Research Achievements through this
Page 59 and 60: Research Achievements IPCS, PPCS, O
Page 61 and 62: Research Achievements protein in co
Page 63 and 64: Research Achievements Investigation
Page 65 and 66: Research Achievements Research on N
Page 67 and 68: Research Achievements Lactobacillus
Page 69 and 70: Research Achievements 1 H-NMR Spect
Page 71 and 72: Research Achievements Production of
Page 73 and 74: Research Achievements Miniaturized
Page 75 and 76: Research Achievements 2 kg/cm ). Op
Page 77 and 78: Research Achievements Selected Whol
Page 79 and 80: Research Achievements 485±32 and 5
Page 81 and 82: Research Achievements from one of t
Page 83 and 84: Research Achievements % NPN, 6 to 1
Page 85 and 86: Research Achievements gulabjamun wi
Page 87 and 88: Research Achievements of frying wer
Page 89 and 90:
Research Achievements The training
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Research Achievements in different
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Sr. No DAIRY EXTENSION DIVISION Fie
Page 95 and 96:
Transfer of Technology Sponsored Co
Page 97 and 98:
Transfer of Technology AGRICULTURE
Page 99 and 100:
Dairy Education Counseling for Admi
Page 101 and 102:
5. HONOURS/AWARDS • A project ent
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Honours/Awards Processed Cheese Spr
Page 105 and 106:
Honours/Awards Mahesh V. Chaudhary
Page 107 and 108:
6. RESEARCH COLLABORATIONS The scie
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Research Collaboration 31. Developm
Page 111 and 112:
7. EMPOWERMENT OF WOMEN & MAINSTREA
Page 113 and 114:
INSTITUTE PUBLICATIONS Aggarwal, An
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Publications and growth curve in Sa
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Publications Kumaresan, A. Johannis
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Publications Salinomycin addition i
Page 121 and 122:
Publications Upadhyay, R. C., Singh
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Publications Dutta, R. (2011) Isola
Page 125 and 126:
Publications antiobesity potential.
Page 127 and 128:
Publications Book Chapter clarifica
Page 129 and 130:
Publications 128-129. Hati, S., Vij
Page 131 and 132:
Publications (2012) Soy based ferme
Page 133 and 134:
Publications Singh, S.K. (2012) Res
Page 135 and 136:
Publications Gupta, Jancy (2012) Da
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Publications Livestock Sci.. 152 :
Page 139 and 140:
Consultancy, Patents and Commercial
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PME the Institute were also documen
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Human Resource Development Dr. S. K
Page 145 and 146:
Human Resource Development Dr. Chan
Page 147 and 148:
Human Resource Development Dr. S. K
Page 149 and 150:
Human Resource Development Dr. Biml
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Human Resource Development Dr. R. K
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13. DISTINGUISHED VISITORS 12.04.20
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14. PERSONNEL INSTITUTE STAFF st As
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Personnel A. K. Chauhan, Ph.D. Prin
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Personnel Veeraju, B.E. (Civil) Tec
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Personnel was promoted to T-9 w.e.f
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15. MAIN STATION RESEARCH DIVISIONS
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Research Division processes for pro
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Research Division methods of manufa
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Support Sections st nd nd rd • Au
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Support Sections Month-wise milking
Page 174 and 175:
Support Sections Reproductive Statu
Page 176 and 177:
Support Sections Production and Pro
Page 178 and 179:
Support Sections Sale of the Dairy
Page 180 and 181:
Support Sections Exhibitions Organi
Page 182 and 183:
16. REGIONAL STATIONS SOUTHERN REGI
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Regional Stations • Training prog
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Regional Stations also converted to
Page 188 and 189:
Regional Stations Production of dif
Page 190 and 191:
Regional Stations • Total 433 liv
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