executive summary - National Dairy Research Institute

Research Achievements
Effect of Selected Herbal Extracts on Levels of
Conjugated Linoleic Acid (CLA) Levels in Milk
on fibre degradation potential by anaerobic rumen
fungi consortium in vitro . Anaerobic rumen fungi
Effect of the selected plant extracts on CLA
consortium was grown using different carbon source
enhancement and important rumen fermentation
viz. cellobiose, glucose, avicel, carboxymethyl
parameters viz., total gas production, individual gases,
cellulose, xylan and wheat straw containing medium to
volatile fatty acid production was studied in vitro
stimulate growth of different fungal isolates. Once the
which was subsequently validated in vivo. There was
consortium was grown, wheat straw (WS) was used as
360% increase in CLA production was observed in case
carbon source for fungal growth. Cultures were
of supplementation with 4% of J. palak plant extract.
incubated for 96 h at 39°C in 125 mL serum bottles
Supplementation of S. nigrum and J. palak. had
under strict anaerobic conditions and various rumen
desirable effects on polyunsaturated fatty acids
fermentation parameters were measured. In addition to
(PUFA), total volatile fatty acids (TVFA) and
different treatments, suitable controls viz. control 1
digestibility under in vitro conditions. In the rumen
(+WS; + Culture; -Feed additive), control 2 (+WS; -
and milk, CLA (32 and 11.7%, P