Qualitative and quantitative analysis of biologically active principles ...
Qualitative and quantitative analysis of biologically active principles ...
Qualitative and quantitative analysis of biologically active principles ...
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Spray reagents<br />
1. Lead acetate<br />
2. Aluminium chloride<br />
3. Benedict’s Reagent<br />
The most suitable spray reagent for coumarin was Benedict’s<br />
Reagent.<br />
The powder <strong>of</strong> each sample was extracted with methanol, ethanol,<br />
chlor<strong>of</strong>orm <strong>and</strong> acetone, fractioned through thin layer<br />
chromatography with various solvent systems <strong>and</strong> analyzed for<br />
psoralen. These plates were sprayed with Benedict’s Reagent.<br />
Spectrophotometric <strong>analysis</strong><br />
The fractions eluted from TLC plates were used for the absorbance<br />
wavelength using a spectrophotometer. The results obtained were<br />
analysed with the s<strong>of</strong>tware.<br />
HPLC <strong>analysis</strong><br />
The presence <strong>of</strong> psoralen detected by medium pressure liquid<br />
chromatography in Aegle marmelos <strong>and</strong> Murraya koenigii was<br />
confirmed by High Pressure Liquid Chromatography (HPLC,<br />
Shimadzu, Japan) using st<strong>and</strong>ard psoralen (Sigma Aldrich).<br />
2.1.3. Screening for luteolin<br />
Selected species <strong>and</strong> their pr<strong>of</strong>ile<br />
Premna serratifolia L. (Plate 3e)<br />
Local name: Munja, Kozhichedi<br />
Small trees or large shrubs. Leaves simple, opposite, lamina 2.5<br />
8.5 x 27.2 cm, elliptic, ellipticoblong, base acute, obtuse,<br />
15
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