Qualitative and quantitative analysis of biologically active principles ...

When the absorbance profiles of different extracts (Figure 17) were
compared, Aegle marmelos and Murraya koenigii showed more or
less similar optical density pattern in methanolic, ethanolic,
chloroform and acetone extracts to the standard. In methanolic
extract, Murraya koenigii gave better results than Aegle marmelos.
Chloroform and acetone extracts did not give much yield when
compared to the other two species. But Aegle marmelos and
Murraya koenigii showed better profile. Paramignya monophylla and
Glycosmis pentaphylla gave similar results with each other but
deviated from the absorbance value of the standard. Clausena
indica produced a comparatively different absorption profile.
The presence of psoralen in Aegle marmelos and Murraya koenigii
detected by medium pressure liquid chromatography was confirmed
by HPLC.
The chloroform and acetone extracts of both the plants were
analysed in HPLC (Shimadzu, Japan). The separation was
performed using reverse–phase C18 column at 25 o C with a flow
rate of 1 ml/min of the solvent actonitril. The absorbance for the
psoralen was monitored at 288 nm with a UV detector SPD­10A
and CLASS VP software. The retention time of standard psoralen
(Sigma) was 4.7 min. Peaks in the same retention time (4.7 min)
were also detected in chloroform as well as acetone extracts of Aegle
marmelos and Murraya koenigii (Figures 18 and 19).
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