Qualitative and quantitative analysis of biologically active principles ...

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Figure 21. In vitro antioxidant analysis of Premna serratifolia. SOD Super oxide, HOD Hydroxyl radical, NONitric oxide, LP Lipid peroxidation Nitric oxide radical scavenging activity Nitric oxide radical generated from sodium nitroprusside at physiological pH was found to be inhibited by the Premna serratifolia root extract. Concentration of 70% methanolc extract needed for 50% of inhibition of nitric oxide radical was 79 µg/ml (Figure 21). Lipid peroxidation activity The root extract was found to inhibit lipid peroxides generated by induction of Fe 2+ /ascorbate and Fe 3+ /ADD/ascorbate in rat liver homogenate. Concentration of methanolic extract needed for 50% inhibition of lipid peroxides was 60 µg/ml (Figure 21). 47
Antiinflammatory activity The administration of the root extract inhibited the carragenan induced acute paw oedema and formalin induced chronic paw oedema in a dose dependent manner (Table 6). The animals treated with 70 per cent methonolic extract @ 250 mg/kg and 500 mg/kg showed 47.05 and 70.58% inhibition of carragenan induced acute inflammation. In the case of formalin induced chronic inflammation, 250 mg/kg and 500 mg/kg showed inhibition of 50.00 and 72.00%, respectively (Table 7). Table 6. Effect of Premna serratifolia (70% methanolic extract) on carrageenan induced paw odema Initial paw Groups thickness (mm) Control 0.21 ± 0.01 Dichlofenac 0.22 ± 0.02 Paw thickness on 3 hrs (mm) Increase in paw thickness % of inhibition 0.38 ± 0.02 0.17 ……. 0.29 ± 0.07 58.82 0.008 a Premna serratifolia 250 mg/kg body wt Premna serratifolia 500 mg/kg body wt 0.21 ± 0.02 0.20 ± 0.01 0.30 ± 0.09 47.05 0.008 a 0.25 ± 0.05 70.58 0.027 a Values are Mean ± SD; for six animals in each group; (a) p, 0.01, (b) p
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KFRI Research Report No. 350 ISBN N
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Contents Acknowledgements Abstract
Page 6 and 7: Abstract In the present study, anal
Page 8 and 9: Among plantderived chemicals, the
Page 10 and 11: eported. Considering the presence o
Page 12 and 13: study from Japan, researchers went
Page 14 and 15: 2. Materials and methods 2.1. Phyto
Page 16: entire, membranous, glabrous. Flowe
Page 19 and 20: Extraction from Scutellaria The pla
Page 23 and 24: tritetra carpellary. Fruit a berr
Page 25 and 26: subcordate or rounded, apex acumina
Page 27 and 28: adical generated by the Fe 3+ /asco
Page 29 and 30: 2.3. Statistical analysis The value
Page 31 and 32: collected elutes were also subjecte
Page 33 and 34: Figure 6. Absorption spectra of Ste
Page 35 and 36: Figure 8. Absorption spectra of Rad
Page 37 and 38: The presence of baicalein in Scutel
Page 39 and 40: Psoralen content TLC analysis The m
Page 41 and 42: Figure 13. TLC comparison of Aegl
Page 43 and 44: Aegle marmelos and Murraya koenigii
Page 45 and 46: A. Comparison of acetone extracts M
Page 47 and 48: C. Comparison of methanol extracts
Page 49 and 50: When the absorbance profiles of dif
Page 51 and 52: Figure 19. HPLC analysis profiles o
Page 53 and 54: Hydroxyl radical scavenging activit
Page 55: colebrookiana and S. violacea posse
Page 59 and 60: adicals by directly scavenging them
Page 61 and 62: eported that psoralen induces inter
Page 63 and 64: Curtin J.F., Donovan M. and Cotter
Page 65 and 66: Patwardhan B., Ashok D.B. and Mukun
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