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D. Comparison of chloroform extracts Murraya koenigii Aegle marmelos Clausena indica Paramignya monophylla Glycosmis pentaphylla 39
When the absorbance profiles of different extracts (Figure 17) were compared, Aegle marmelos and Murraya koenigii showed more or less similar optical density pattern in methanolic, ethanolic, chloroform and acetone extracts to the standard. In methanolic extract, Murraya koenigii gave better results than Aegle marmelos. Chloroform and acetone extracts did not give much yield when compared to the other two species. But Aegle marmelos and Murraya koenigii showed better profile. Paramignya monophylla and Glycosmis pentaphylla gave similar results with each other but deviated from the absorbance value of the standard. Clausena indica produced a comparatively different absorption profile. The presence of psoralen in Aegle marmelos and Murraya koenigii detected by medium pressure liquid chromatography was confirmed by HPLC. The chloroform and acetone extracts of both the plants were analysed in HPLC (Shimadzu, Japan). The separation was performed using reverse–phase C18 column at 25 o C with a flow rate of 1 ml/min of the solvent actonitril. The absorbance for the psoralen was monitored at 288 nm with a UV detector SPD10A and CLASS VP software. The retention time of standard psoralen (Sigma) was 4.7 min. Peaks in the same retention time (4.7 min) were also detected in chloroform as well as acetone extracts of Aegle marmelos and Murraya koenigii (Figures 18 and 19). 40
Page 2 and 3: KFRI Research Report No. 350 ISBN N
Page 4 and 5: Contents Acknowledgements Abstract
Page 6 and 7: Abstract In the present study, anal
Page 8 and 9: Among plantderived chemicals, the
Page 10 and 11: eported. Considering the presence o
Page 12 and 13: study from Japan, researchers went
Page 14 and 15: 2. Materials and methods 2.1. Phyto
Page 16: entire, membranous, glabrous. Flowe
Page 19 and 20: Extraction from Scutellaria The pla
Page 23 and 24: tritetra carpellary. Fruit a berr
Page 25 and 26: subcordate or rounded, apex acumina
Page 27 and 28: adical generated by the Fe 3+ /asco
Page 29 and 30: 2.3. Statistical analysis The value
Page 31 and 32: collected elutes were also subjecte
Page 33 and 34: Figure 6. Absorption spectra of Ste
Page 35 and 36: Figure 8. Absorption spectra of Rad
Page 37 and 38: The presence of baicalein in Scutel
Page 39 and 40: Psoralen content TLC analysis The m
Page 41 and 42: Figure 13. TLC comparison of Aegl
Page 43 and 44: Aegle marmelos and Murraya koenigii
Page 45 and 46: A. Comparison of acetone extracts M
Page 47: C. Comparison of methanol extracts
Page 51 and 52: Figure 19. HPLC analysis profiles o
Page 53 and 54: Hydroxyl radical scavenging activit
Page 55 and 56: colebrookiana and S. violacea posse
Page 57 and 58: Antiinflammatory activity The adm
Page 59 and 60: adicals by directly scavenging them
Page 61 and 62: eported that psoralen induces inter
Page 63 and 64: Curtin J.F., Donovan M. and Cotter
Page 65 and 66: Patwardhan B., Ashok D.B. and Mukun

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