Qualitative and quantitative analysis of biologically active principles ...
Qualitative and quantitative analysis of biologically active principles ...
Qualitative and quantitative analysis of biologically active principles ...
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Psoralen content<br />
TLC <strong>analysis</strong><br />
The methanolic, ethanolic, chlor<strong>of</strong>orm <strong>and</strong> acetone extracts <strong>of</strong> fruits<br />
along with control were subjected to thin layer chromatography<br />
using various solvent systems. The most suitable solvent system<br />
was toluene: petroleum benzene: acetic acid (4.4: 4.4: 1.2).<br />
Pr<strong>of</strong>iles obtained for samples show more or less similar patterns <strong>of</strong><br />
b<strong>and</strong>s with control (Table 3). When ethanolic extract was used,<br />
separation <strong>and</strong> intensity <strong>of</strong> b<strong>and</strong>s were more.<br />
Table 3. Detection based on intensities <strong>of</strong> brownish b<strong>and</strong>s<br />
Aegle<br />
marmelos<br />
Murraya<br />
koenigii<br />
Clausena<br />
indica<br />
Paramignya<br />
monophylla<br />
Glycosmis<br />
pentaphylla<br />
Plants<br />
Intensities<br />
<strong>of</strong><br />
brownish<br />
b<strong>and</strong>s<br />
St<strong>and</strong>arde<br />
d<br />
Dark<br />
brown<br />
Dark<br />
brown<br />
Dark<br />
brown<br />
Dark<br />
brown<br />
Dark<br />
brown<br />
Samples taken for study<br />
Methanol Ethanol Chlor<strong>of</strong>orm Acetone<br />
Light<br />
brown<br />
Light<br />
brown<br />
Light<br />
greenish<br />
brown<br />
Fleshy<br />
Light<br />
greenish<br />
brown<br />
Dark<br />
brown<br />
Dark<br />
brown<br />
Greenis<br />
h brown<br />
Dark<br />
fleshy<br />
Greenis<br />
h brown<br />
Brown<br />
Brown<br />
Greenish<br />
brown<br />
Brown<br />
Brown<br />
Light<br />
greenis<br />
h brown<br />
Dark fleshy Fleshy<br />
Greenish<br />
brown<br />
Light<br />
greenis<br />
h brown<br />
By spraying Benedict’s Reagent, different b<strong>and</strong>ing patterns were<br />
obtained in all the samples used (Table 4). Aegle marmelos <strong>and</strong><br />
Murraya koenigii (Figure 11) showed more or less same number <strong>of</strong><br />
b<strong>and</strong>s. When Paramignya monophylla <strong>and</strong> Clausena indica compared<br />
in TLC <strong>analysis</strong>, the results were entirely different (Figure 12). Aegle<br />
marmelos <strong>and</strong> Glycosmis pentaphylla in TLC <strong>analysis</strong> showed<br />
difference in the number <strong>of</strong> b<strong>and</strong>s (Figure 13). Murraya koenigii <strong>and</strong><br />
Glycosmis pentaphylla did not show any resemblances (Figure 14).<br />
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