Qualitative and quantitative analysis of biologically active principles ...

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Figure 4. Absorption spectra of various solvent extracts of Oroxylum indicum isolated through column chromatography The different fractions eluted from the column were then subjected to spectrophotometric analysis. The wavelength ranging from 200– 900 nm was selected for generating the spectra (Figure 58). Among the fractions screened, the fraction obtained from Scutellaria spp. Alone showed the peaks identical with standard baicalein. The 21
collected elutes were also subjected to TLC with various solvent systems and the baicalien content was detected by spraying Benedict’s reagent (Figure 9). The Rf. value of standard baicalein and samples from Scutellaria was equal. Table 1. Weight of the fractions obtained in ethyl acetate by soxhlet extraction No Sample mg.l 1 Stereospermum suaveolens 451 2 Stereospermum colais 599 3 Dolichandrone arcuata 499 4 Radermachera xylocarpa 428 5 Millingtonia hortensis 211 6 Scutellaria colebrookiana 227 7 Scutellaria violacea 271 Table 2. The quantity and % yield of different elutes obtained through silica gel column No Sample (1mg) 1 st Elute 2 nd Elute 3 rd Elute 4 th Elute 5 th Elute 1 Stereospermum suaveolens 0.166 mg (16.6%) 0.151 mg (15.1%) 0.199 mg (19.9%) 0.140 mg (14%) 0.140 mg (14%) 2 Stereospermum colais 0.166 mg (16.6%) 0.153 mg (15.3%) 0.155 mg (15.5%) 0.145 mg 14.5%) 0.133 mg 13.3%) 3 Dolichandrone arcuata 0.124 mg (12.4%) 0.170 mg (17.0%) 0.154 mg (15.4%) 0.258 mg (25.8%) 0.187 mg (18.7%) 4 Radermachera xylocarpa 0.163 mg (16.3%) 0.121 mg (12.1%) 0.189 mg (18.9%) 0.173 mg (17.3%) 0.239 mg (23.9%) 5 Millingtonia hortensis 0.063 mg (6.3%) 0.160 mg (16%) 0.149 mg (14.9%) 0.243 mg (24.3%) 0.181 mg (18.1%) 6 Scutellaria colebrookiana 0.056 mg (5.6%) 0.102 mg (10.2%) 0.156 mg (15.6%) 0.122 mg (12.2%) 0.098 mg (9.8%) 7 Scutellaria violacea 0.121 mg (12.1%) 0.217 mg (21.7%) 0.176 mg (17.6%) 0.162 mg (16.2%) 0.133 mg (13.3%) 22
Page 2 and 3: KFRI Research Report No. 350 ISBN N
Page 4 and 5: Contents Acknowledgements Abstract
Page 6 and 7: Abstract In the present study, anal
Page 8 and 9: Among plantderived chemicals, the
Page 10 and 11: eported. Considering the presence o
Page 12 and 13: study from Japan, researchers went
Page 14 and 15: 2. Materials and methods 2.1. Phyto
Page 16: entire, membranous, glabrous. Flowe
Page 19 and 20: Extraction from Scutellaria The pla
Page 23 and 24: tritetra carpellary. Fruit a berr
Page 25 and 26: subcordate or rounded, apex acumina
Page 27 and 28: adical generated by the Fe 3+ /asco
Page 29: 2.3. Statistical analysis The value
Page 33 and 34: Figure 6. Absorption spectra of Ste
Page 35 and 36: Figure 8. Absorption spectra of Rad
Page 37 and 38: The presence of baicalein in Scutel
Page 39 and 40: Psoralen content TLC analysis The m
Page 41 and 42: Figure 13. TLC comparison of Aegl
Page 43 and 44: Aegle marmelos and Murraya koenigii
Page 45 and 46: A. Comparison of acetone extracts M
Page 47 and 48: C. Comparison of methanol extracts
Page 49 and 50: When the absorbance profiles of dif
Page 51 and 52: Figure 19. HPLC analysis profiles o
Page 53 and 54: Hydroxyl radical scavenging activit
Page 55 and 56: colebrookiana and S. violacea posse
Page 57 and 58: Antiinflammatory activity The adm
Page 59 and 60: adicals by directly scavenging them
Page 61 and 62: eported that psoralen induces inter
Page 63 and 64: Curtin J.F., Donovan M. and Cotter
Page 65 and 66: Patwardhan B., Ashok D.B. and Mukun

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