XII Iberian Meeting of Electrochemistry XVI Meeting of the ...

XII Iberian Meeting of Electrochemistry & XVI Meeting of the Portuguese Electrochemical Society PL 04
Dynamic Electrochemical Studies of Redox Proteins
Margarida M. Correia dos Santos
Centro de Química Estrutural, IST, Av. Rovisco Pais, 1049-001, Lisboa, Portugal
mcsantos@ist.utl.pt
The use of voltammetric techniques is well proved nowadays in unravelling important
aspects of the chemistry of metalloproteins and metalloenzymes [1,2]. Unlike the more
conventional and widespread used of potenciometric titrations, voltammetric methods
allow in situ measurements of reduction potentials together with the acquisition of
information about the kinetics of the electrode reactions as well of relevant parameters
of coupled reactions including catalysis.
It is now well recognized the electron transfer process occurring between a redox protein
and a solid electrode is a protein electrode surface recognition process A large effort has
been put forward to understand and control the way the protein surface interacts with the
electrodes and nowadays the problem is quite mastered.
advantages over bulk titrations. However a wealth of information can be obtained by
these methodologies that quite overpass the "static" utilization of the technique. The
study of electron transfer (intra and intermolecular), recognition of redox partners and
the observation of catalytic currents, for example, puts the methods on a dynamic range
of utilization.
Some of these topics will be covered in this lecture [3-8].
References
[1] Armstrong, F.A.; Bioelectrochemistry (Ed.: Wilson G..S.) in Encyclopedia of Electrochemistry
(Eds: Bard, A..J.;.Stratmann, M.)., Vol.9, Ch.1, 13, Wiley-VCH, 2003.
[2] Léger, C.; Bertrand, P. Chem.Rev., 2008, 108, 2379.
[3] Santos, M.M.C.; de Sousa, P.M.P.; Gonçalves, M.L.S.; Krippahl, L.; Moura, J.J.G.; Lojou, E. ;
Bianco, P. J. Electroanal. Chem., 2003, 541, 153.
[4] Santos, M.M.C.; de Sousa, P.M.P.; Gonçalves, M.L.S.; Romão, M..J.; Moura, I. ; Moura, J.J.G.
Eur. J. Biochem., 2004, 271, 1329.
[5] Santos, M.; Santos, M.M.C.; Gonçalves, M.L.S.; Costa, C.; Romão, J.C.; Moura, J.J.G. J. Inorg.
Biochem., 2006, 100, 2009.
[6] de Sousa, P.M.P.; Pauleta, S.R.; Gonçalves, M.L.S.; Pettigrew, G.W.; Moura, I.; Santos,
M.M.C.; Moura, J.J.G. J Biol Inorg Chem., 2007, 12, 691.
[7] de Sousa, P.M.P.; Pauleta, S.R.; Rodrigues, D.; Gonçalves, M.L.S.; Pettigrew, G.W.; Moura, I.;
Moura, J.J.G.; Santos, M.M.C. J Biol Inorg Chem., 2008, 13, 779.
[8] de Sousa, P.M.P.; Pauleta, S.R.; Gonçalves, M.L.S.; Pettigrew, G.W.; Moura, I.; Moura, J.J.G.;
Santos, M.M.C. Submitted for publication in Angewandte Chemie.
September, 811, 2010. ISEL - Lisbon 15