BD FACSCanto Clinical Software Reference Manual

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Chapter 6: Troubleshooting 119 General Software Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 120 Setup Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 Setup Wizard Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126 Setup Report Failure Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Levey-Jennings Errors and Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Acquisition Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134 Analysis Troubleshooting for 4- and 6-Color TBNK . . . . . . . . . . . . . . . . . . . 138 QC Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 4- and 6-Color TBNK Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143 Disabling the Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146 Appendix A: Menus and Keyboard Shortcuts 147 Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148 Keyboard Shortcuts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150 Menu Command Shortcuts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 Appendix B: Technical Overview for BD Multitest 4- and 6-Color Reagents 155 Panels and Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156 Acquisition Stopping Criteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Gate Hierarchy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158 Visual Check for BD Multitest Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163 Default Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 Options Defaults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168 Reagent Defaults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170 Alarm Ranges Defaults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175 Report Defaults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176 Lab Manager Preferences Defaults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178 Index 181 vi BD FACSCanto Clinical Software Reference Manual
About This Manual This manual contains reference information about BD FACSCanto clinical software. Designed for the BD FACSCanto and the BD FACSCanto II flow cytometers, the software handles setup, acquisition, analysis, and automated loading of samples. For information about cytometer components, maintenance, and troubleshooting, and instructions on how to run samples using the software, refer to the printed instructions for use for either the BD FACSCanto or the BD FACSCanto II flow cytometer. Further details can be found in the reference manual, provided on the documentation CD, for either the BD FACSCanto or the BD FACSCanto II flow cytometer. For application-specific instructions, refer to the individual application guides and information supplied with the reagents. The BD FACSCanto Clinical Software Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar with the Windows operating system, refer to the documentation provided with your computer. Before using BD FACSCanto clinical software, print and review the ReadMe file for BD FACSCanto clinical software that is included on both the software and documentation CD. It contains important information that is not included in the printed or electronic documentation. vii
Page 1 and 2: BD FACSCanto Clinical Software Refe
Page 3 and 4: Contents About This Manual Conventi
Page 5: Disabling User Accounts . . . . . .
Page 9 and 10: Technical Assistance For technical
Page 11 and 12: 1 Introduction BD FACSCanto clinica
Page 13 and 14: Compatibility Data Files BD FACSCan
Page 15 and 16: 2 Starting Up Use this chapter to f
Page 17 and 18: minimize maximize close menu bar to
Page 19 and 20: Becoming Familiar with Toolbars Too
Page 21 and 22: Viewing Status Indicators Status wi
Page 23 and 24: Parameter Acceptable Range Details
Page 25 and 26: Becoming Familiar with the Worklist
Page 27 and 28: Deleting Samples from a Worklist 1
Page 29 and 30: Understanding the Workflow The foll
Page 31 and 32: 3 Reports The software generates fo
Page 33 and 34: Figure 3-1 Cytometer Setup Report (
Page 35 and 36: 7 Comments—Provides an area to wr
Page 37 and 38: 3 4 5 6 Detectors—Shows either BD
Page 39 and 40: 3 All Levey-Jennings plots contain
Page 41 and 42: 1 2 3 A lab manager chooses which R
Page 43 and 44: 4 User Options Certain software opt
Page 45 and 46: Entering Lot Information for Setup
Page 47 and 48: Figure 4-3 Example setup beads labe
Page 49 and 50: 2D Barcode Symbologies BD Bioscienc
Page 51 and 52: The lot ID, expiration date, target
Page 53 and 54: Connecting to the Cytometer After s
Page 55 and 56: Opening an Existing Worklist 1 Sele
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Importing a Worklist from BD FACS S
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Printing a Worklist You can print a
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Exporting the Worklist to Another F
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Printing Setup Reports Automaticall
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Specifying a Display Time for the L
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Enter a new location or find a loca
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Figure 4-5 Example Lab Report A dia
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Figure 4-6 Location of current file
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5 Lab Manager Options In BD FACSCan
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Keep the default of Anyone who uses
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The installer loads the software an
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The installer places the software a
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Table 5-1 Files (continued) File Ty
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Changing Default File Locations The
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Managing User Accounts • Setting
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The default name (User 1) appears o
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3 Click Delete User. 4 Click Yes to
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Option Automatically when connectin
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Specifying Levey-Jennings View Pref
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For example, a choice of +/- 3SD me
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Changing Worklist Report Header Pre
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Changing Acquisition Targets To cha
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4 Click OK. Changing the Lab Report
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• Disabling Comments on page 110
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Subset results choices will vary, d
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4 Select the QC results you want to
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If the lymphosum falls beyond the d
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3 Select the header items you want
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5 Click OK. Other Options You can m
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• Click Move Up or Move Down unti
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6 (Optional) Click Preview to view
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6 Troubleshooting The tips in this
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General Software Troubleshooting (c
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General Software Troubleshooting (c
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Setup Troubleshooting • For help
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Setup Wizard Messages (continued) M
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Setup Wizard Messages (continued) M
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Setup Report Failure Messages (cont
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Levey-Jennings Errors and Messages
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Acquisition Troubleshooting (contin
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Acquisition Troubleshooting (contin
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QC Messages (continued) Message Pos
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QC Messages (continued) Message Pos
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4- and 6-Color TBNK Troubleshooting
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4- and 6-Color TBNK Troubleshooting
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Appendix A Menus and Keyboard Short
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Tools menu, all users Tools menu, l
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Menu Command Shortcuts To select me
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Function Keys Use the function keys
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Appendix B Technical Overview for B
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Acquisition Stopping Criteria The f
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3/8/45/4 Plot Population(s) of Inte
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3/16+56/45/19 Plot Population(s) of
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Visual Check for BD Multitest Reage
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2 Inspect the CD4 vs SSC plot. For
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• Merged clusters If you are stil
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Preference Menu Item Value Files Fi
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4-Color Multitest (continued) Reage
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4-Color Multitest TruC (continued)
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Alarm Ranges Defaults Only lab mana
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Report Option Enable Comments Secti
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Preference Menu Item Value Results
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Index Symbols 26 % T-sum about 108
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files BD FACSCanto 80 compatibility
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Q QC messages 107, 138 values 106,
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users adding 85 deleting 88 disabli
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