Principles and Practices of Biosafety - San Diego State University

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BSL 1: Work Practices and Procedures • Applications Non-infectious agent and tissue culture, media preparation • Prevent Cross Contamination Keep cultures covered Flame instruments and containers Use sterile media and equipment Keep hands or face away from cultures
BSL 1: Work Practices and Procedures • Biosafety Procedures Work with agents may be conducted on open bench Wash hands often No mouth pipetting No eating or drinking in lab Minimize aerosol generation Decontaminate work surfaces Wear applicable PPE
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Principles and Practices of Biosafe
Page 3 and 4:
Introduction The management of biol
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SDSU Biosafety Requirements and Gui
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Institutional Biosafety Committee a
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Submission of New BUA • New infec
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Principal Investigator • Develops
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Risk Group and Biosafety Level
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Classification of Infective Agents
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Risk Group and Biosafety Level Clas
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Introduction • California Code of
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Exposure Determination • Exposure
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Hepatitis B Vaccination and Post-Ex
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Pathogenic Microbiology
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Viral Laboratory Acquired Infection
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Molecular Biology
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Risk Assessment The following risk
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Donor Organism and Cloned DNA Inser
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Transgenic and “Knock-Out” Anim
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Human Source Material • Blood and
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Cell Culture Risks • Contaminatin
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Cell Culture Safety • Extend Univ
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Animals and Allergens
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Risks Associated with the Agent Use
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Transmission of Biohazards During W
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Transmission of Biohazards During W
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Risk Reduction: Containment of Infe
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Containment Caging Systems • Micr
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Containment Caging Systems • Can
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Special Animal Housing Situations
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Some Animals and Their Zoonoses •
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Transmission of Zoonoses • Enteri
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Laboratory Acquired Allergies (LAA)
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Routes of Exposure to LAA • Inhal
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LAA: Exposure Control • Engineeri
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Why Disinfect • To reduce or elim
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Classes of Disinfectants • Chlori
Page 73 and 74: • Organic Load Blood, sputum, mil
Page 75 and 76: • Contact Time Disinfectants shou
Page 77 and 78: Medical Waste Disposal (Biohazardou
Page 79 and 80: Medical Waste …biohazardous waste
Page 81 and 82: Medical Waste Does Not Include: •
Page 83 and 84: Sharps Waste … means any device h
Page 85 and 86: Biohazard Bag …means a disposable
Page 87 and 88: • Containers Containers shall be
Page 89 and 90: Containment and Storage Sharps Wast
Page 91 and 92: Solid Medical Waste Collection Must
Page 93 and 94: What’s Wrong with these Pictures
Page 101 and 102: Containment Equipment and Facilitie
Page 103 and 104: Primary Barrier • Primary barrier
Page 105 and 106: Primary Barriers - Equipment • Pe
Page 107 and 108: Chemical Fume Hood • 100 fpm face
Page 109 and 110: Clean Bench / Laminar Flow Hoods
Page 111 and 112: Class I Cabinet • 75 fpm face vel
Page 113 and 114: Any Comments BSC - remove unnecessa
Page 115 and 116: Types of Biosafety Cabinets NSF/ANS
Page 117 and 118: Other Primary Barriers- Engineering
Page 119 and 120: Biosafety Practices and Procedures
Page 121 and 122: Administrative Controls • Substit
Page 123: Personal Protective Clothing and Eq
Page 127 and 128: BSL 2: Work Practices and Procedure
Page 129 and 130: Correct Use of Biosafety Cabinets
Page 131 and 132: Correct Use of Biosafety Cabinets
Page 133 and 134: Safe Use of Centrifuge • Use seal
Page 135 and 136: Minimizing Aerosols • Use careful
Page 137 and 138: Use Extreme Care with Sharps • Us
Page 139 and 140: Signs and Labels
Page 141 and 142: Biohazard Label • Must be attache
Page 143 and 144: It is important that the laboratory
Page 145 and 146: Moving the Biosafety Cabinet • Di
Page 147 and 148: Shipment and Transportation
Page 149 and 150: Emergency Response to Biological In
Page 151 and 152: Exposure Management For needlestick
Page 153 and 154: Biological Spill Clean-Up Kit- Basi
Page 155 and 156: Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158: Biosecurity
Page 159 and 160: Inspection
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Principles and Practices of Biosafety - San Diego State University

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