Principles and Practices of Biosafety - San Diego State University

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Biohazardous Waste • Laboratory Wastes Human or animal specimen cultures Cultures and stocks of infectious agents Wastes from production of biologicals, live and attenuated vaccines, culture dishes and devices • Pharmaceuticals • Human surgery specimens or tissues, including those fixed in fixatives • Animal parts, tissues, fluids, or carcasses • Wastes containing recognizable fluid blood or blood products, containers with fluid blood, blood from animals • Wastes contaminated or containing chemotherapeutic agents
Sharps Waste … means any device having acute rigid corners, edges, or protuberances capable of cutting or piercing. • Needles, needles with syringes, contaminated syringes, blades, needles with attached tubing • Broken glass items i.e., Pasteur pipettes, blood vials contaminated with biohazardous waste
Page 1 and 2:
Principles and Practices of Biosafe
Page 3 and 4:
Introduction The management of biol
Page 5 and 6:
SDSU Biosafety Requirements and Gui
Page 7 and 8:
Institutional Biosafety Committee a
Page 9 and 10:
Submission of New BUA • New infec
Page 11 and 12:
Principal Investigator • Develops
Page 13 and 14:
Risk Group and Biosafety Level
Page 15 and 16:
Classification of Infective Agents
Page 17 and 18:
Risk Group and Biosafety Level Clas
Page 19 and 20:
Introduction • California Code of
Page 21 and 22:
Exposure Determination • Exposure
Page 23 and 24:
Hepatitis B Vaccination and Post-Ex
Page 25 and 26:
Pathogenic Microbiology
Page 27 and 28:
Viral Laboratory Acquired Infection
Page 29 and 30:
Molecular Biology
Page 31 and 32: Risk Assessment The following risk
Page 33 and 34: Donor Organism and Cloned DNA Inser
Page 35 and 36: Transgenic and “Knock-Out” Anim
Page 37 and 38: Human Source Material • Blood and
Page 39 and 40: Cell Culture Risks • Contaminatin
Page 41 and 42: Cell Culture Safety • Extend Univ
Page 43 and 44: Animals and Allergens
Page 45 and 46: Risks Associated with the Agent Use
Page 47 and 48: Transmission of Biohazards During W
Page 49 and 50: Transmission of Biohazards During W
Page 51 and 52: Risk Reduction: Containment of Infe
Page 53 and 54: Containment Caging Systems • Micr
Page 55 and 56: Containment Caging Systems • Can
Page 57 and 58: Special Animal Housing Situations
Page 59 and 60: Some Animals and Their Zoonoses •
Page 61 and 62: Transmission of Zoonoses • Enteri
Page 63 and 64: Laboratory Acquired Allergies (LAA)
Page 65 and 66: Routes of Exposure to LAA • Inhal
Page 67 and 68: LAA: Exposure Control • Engineeri
Page 69 and 70: Why Disinfect • To reduce or elim
Page 71 and 72: Classes of Disinfectants • Chlori
Page 73 and 74: • Organic Load Blood, sputum, mil
Page 75 and 76: • Contact Time Disinfectants shou
Page 77 and 78: Medical Waste Disposal (Biohazardou
Page 79 and 80: Medical Waste …biohazardous waste
Page 81: Medical Waste Does Not Include: •
Page 85 and 86: Biohazard Bag …means a disposable
Page 87 and 88: • Containers Containers shall be
Page 89 and 90: Containment and Storage Sharps Wast
Page 91 and 92: Solid Medical Waste Collection Must
Page 93 and 94: What’s Wrong with these Pictures
Page 101 and 102: Containment Equipment and Facilitie
Page 103 and 104: Primary Barrier • Primary barrier
Page 105 and 106: Primary Barriers - Equipment • Pe
Page 107 and 108: Chemical Fume Hood • 100 fpm face
Page 109 and 110: Clean Bench / Laminar Flow Hoods
Page 111 and 112: Class I Cabinet • 75 fpm face vel
Page 113 and 114: Any Comments BSC - remove unnecessa
Page 115 and 116: Types of Biosafety Cabinets NSF/ANS
Page 117 and 118: Other Primary Barriers- Engineering
Page 119 and 120: Biosafety Practices and Procedures
Page 121 and 122: Administrative Controls • Substit
Page 123 and 124: Personal Protective Clothing and Eq
Page 125 and 126: BSL 1: Work Practices and Procedure
Page 127 and 128: BSL 2: Work Practices and Procedure
Page 129 and 130: Correct Use of Biosafety Cabinets
Page 131 and 132: Correct Use of Biosafety Cabinets
Page 133 and 134:
Safe Use of Centrifuge • Use seal
Page 135 and 136:
Minimizing Aerosols • Use careful
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Use Extreme Care with Sharps • Us
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Signs and Labels
Page 141 and 142:
Biohazard Label • Must be attache
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It is important that the laboratory
Page 145 and 146:
Moving the Biosafety Cabinet • Di
Page 147 and 148:
Shipment and Transportation
Page 149 and 150:
Emergency Response to Biological In
Page 151 and 152:
Exposure Management For needlestick
Page 153 and 154:
Biological Spill Clean-Up Kit- Basi
Page 155 and 156:
Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158:
Biosecurity
Page 159 and 160:
Inspection
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