Principles and Practices of Biosafety - San Diego State University

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Careful Pipetting Techniques • Never blow out last drop in pipette • Use pipetting aids with filters • Never mix by suction and expulsion (mix by sonication) • Discharge liquid down side of container, using tip-to to-wall contact • Deliver as close as possible to contents • Work over plastic-backed absorbent matting (ensure it doesn’t t slide forward or backward blocking air grill)
Use Extreme Care with Sharps • Use sharps if only absolutely required as part of a process • Percutaneous exposure risk Employ safe work practices Utilize safe sharp devices • Aerosol exposure risk Use biosafety cabinet for removal of air from needle • Use mechanical methods for needle removal • Never bend, recap or manipulate sharps by hand • Keep hands away from needle
Page 1 and 2:
Principles and Practices of Biosafe
Page 3 and 4:
Introduction The management of biol
Page 5 and 6:
SDSU Biosafety Requirements and Gui
Page 7 and 8:
Institutional Biosafety Committee a
Page 9 and 10:
Submission of New BUA • New infec
Page 11 and 12:
Principal Investigator • Develops
Page 13 and 14:
Risk Group and Biosafety Level
Page 15 and 16:
Classification of Infective Agents
Page 17 and 18:
Risk Group and Biosafety Level Clas
Page 19 and 20:
Introduction • California Code of
Page 21 and 22:
Exposure Determination • Exposure
Page 23 and 24:
Hepatitis B Vaccination and Post-Ex
Page 25 and 26:
Pathogenic Microbiology
Page 27 and 28:
Viral Laboratory Acquired Infection
Page 29 and 30:
Molecular Biology
Page 31 and 32:
Risk Assessment The following risk
Page 33 and 34:
Donor Organism and Cloned DNA Inser
Page 35 and 36:
Transgenic and “Knock-Out” Anim
Page 37 and 38:
Human Source Material • Blood and
Page 39 and 40:
Cell Culture Risks • Contaminatin
Page 41 and 42:
Cell Culture Safety • Extend Univ
Page 43 and 44:
Animals and Allergens
Page 45 and 46:
Risks Associated with the Agent Use
Page 47 and 48:
Transmission of Biohazards During W
Page 49 and 50:
Transmission of Biohazards During W
Page 51 and 52:
Risk Reduction: Containment of Infe
Page 53 and 54:
Containment Caging Systems • Micr
Page 55 and 56:
Containment Caging Systems • Can
Page 57 and 58:
Special Animal Housing Situations
Page 59 and 60:
Some Animals and Their Zoonoses •
Page 61 and 62:
Transmission of Zoonoses • Enteri
Page 63 and 64:
Laboratory Acquired Allergies (LAA)
Page 65 and 66:
Routes of Exposure to LAA • Inhal
Page 67 and 68:
LAA: Exposure Control • Engineeri
Page 69 and 70:
Why Disinfect • To reduce or elim
Page 71 and 72:
Classes of Disinfectants • Chlori
Page 73 and 74:
• Organic Load Blood, sputum, mil
Page 75 and 76:
• Contact Time Disinfectants shou
Page 77 and 78:
Medical Waste Disposal (Biohazardou
Page 79 and 80:
Medical Waste …biohazardous waste
Page 81 and 82:
Medical Waste Does Not Include: •
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Sharps Waste … means any device h
Page 85 and 86: Biohazard Bag …means a disposable
Page 87 and 88: • Containers Containers shall be
Page 89 and 90: Containment and Storage Sharps Wast
Page 91 and 92: Solid Medical Waste Collection Must
Page 93 and 94: What’s Wrong with these Pictures
Page 101 and 102: Containment Equipment and Facilitie
Page 103 and 104: Primary Barrier • Primary barrier
Page 105 and 106: Primary Barriers - Equipment • Pe
Page 107 and 108: Chemical Fume Hood • 100 fpm face
Page 109 and 110: Clean Bench / Laminar Flow Hoods
Page 111 and 112: Class I Cabinet • 75 fpm face vel
Page 113 and 114: Any Comments BSC - remove unnecessa
Page 115 and 116: Types of Biosafety Cabinets NSF/ANS
Page 117 and 118: Other Primary Barriers- Engineering
Page 119 and 120: Biosafety Practices and Procedures
Page 121 and 122: Administrative Controls • Substit
Page 123 and 124: Personal Protective Clothing and Eq
Page 125 and 126: BSL 1: Work Practices and Procedure
Page 127 and 128: BSL 2: Work Practices and Procedure
Page 129 and 130: Correct Use of Biosafety Cabinets
Page 131 and 132: Correct Use of Biosafety Cabinets
Page 133 and 134: Safe Use of Centrifuge • Use seal
Page 135: Minimizing Aerosols • Use careful
Page 139 and 140: Signs and Labels
Page 141 and 142: Biohazard Label • Must be attache
Page 143 and 144: It is important that the laboratory
Page 145 and 146: Moving the Biosafety Cabinet • Di
Page 147 and 148: Shipment and Transportation
Page 149 and 150: Emergency Response to Biological In
Page 151 and 152: Exposure Management For needlestick
Page 153 and 154: Biological Spill Clean-Up Kit- Basi
Page 155 and 156: Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158: Biosecurity
Page 159 and 160: Inspection
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Principles and Practices of Biosafety - San Diego State University

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