Principles and Practices of Biosafety - San Diego State University

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Correct Use of Biosafety Cabinets – Open Flames • DO NOT use Bunsen burners or open flames Fire hazard Can damage HEPA filter Interferes with proper air flow Microincinerator preferred Burner with pilot light not a good alternative Open flames react with disinfectants (flammables)
Safe Use of Centrifuge • Use sealed tubes, rotors, and safety cups/buckets that are sealed d with O-rings • Inspect tubes, O-rings O and rotors for wear, and buckets for cracks, chips, erosion, etc. • Do not use aluminum foil to cap centrifuge tubes • Clean and maintain gaskets and O-ringsO • Change O-rings O if compromised • Load/unload centrifuge tubes, rotors and accessories in BSC • Small, low speed centrifuges may be placed in a BSC; however, high speed centrifuges pose additional hazards • Do not overfill tubes • Balance buckets, tubes and rotors properly before centrifugation • Wait 5 minutes (or 30 mins. for high speed centrifuge) after each h run before opening • Do not decant or pour off supernatant. Use a vacuum system with appropriate in-line reservoirs and filters
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Principles and Practices of Biosafe
Page 3 and 4:
Introduction The management of biol
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SDSU Biosafety Requirements and Gui
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Institutional Biosafety Committee a
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Submission of New BUA • New infec
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Principal Investigator • Develops
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Risk Group and Biosafety Level
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Classification of Infective Agents
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Risk Group and Biosafety Level Clas
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Introduction • California Code of
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Exposure Determination • Exposure
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Hepatitis B Vaccination and Post-Ex
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Pathogenic Microbiology
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Viral Laboratory Acquired Infection
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Molecular Biology
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Risk Assessment The following risk
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Donor Organism and Cloned DNA Inser
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Transgenic and “Knock-Out” Anim
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Human Source Material • Blood and
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Cell Culture Risks • Contaminatin
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Cell Culture Safety • Extend Univ
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Animals and Allergens
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Risks Associated with the Agent Use
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Transmission of Biohazards During W
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Transmission of Biohazards During W
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Risk Reduction: Containment of Infe
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Containment Caging Systems • Micr
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Containment Caging Systems • Can
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Special Animal Housing Situations
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Some Animals and Their Zoonoses •
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Transmission of Zoonoses • Enteri
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Laboratory Acquired Allergies (LAA)
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Routes of Exposure to LAA • Inhal
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LAA: Exposure Control • Engineeri
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Why Disinfect • To reduce or elim
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Classes of Disinfectants • Chlori
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• Organic Load Blood, sputum, mil
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• Contact Time Disinfectants shou
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Medical Waste Disposal (Biohazardou
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Medical Waste …biohazardous waste
Page 81 and 82: Medical Waste Does Not Include: •
Page 83 and 84: Sharps Waste … means any device h
Page 85 and 86: Biohazard Bag …means a disposable
Page 87 and 88: • Containers Containers shall be
Page 89 and 90: Containment and Storage Sharps Wast
Page 91 and 92: Solid Medical Waste Collection Must
Page 93 and 94: What’s Wrong with these Pictures
Page 101 and 102: Containment Equipment and Facilitie
Page 103 and 104: Primary Barrier • Primary barrier
Page 105 and 106: Primary Barriers - Equipment • Pe
Page 107 and 108: Chemical Fume Hood • 100 fpm face
Page 109 and 110: Clean Bench / Laminar Flow Hoods
Page 111 and 112: Class I Cabinet • 75 fpm face vel
Page 113 and 114: Any Comments BSC - remove unnecessa
Page 115 and 116: Types of Biosafety Cabinets NSF/ANS
Page 117 and 118: Other Primary Barriers- Engineering
Page 119 and 120: Biosafety Practices and Procedures
Page 121 and 122: Administrative Controls • Substit
Page 123 and 124: Personal Protective Clothing and Eq
Page 125 and 126: BSL 1: Work Practices and Procedure
Page 127 and 128: BSL 2: Work Practices and Procedure
Page 129 and 130: Correct Use of Biosafety Cabinets
Page 131: Correct Use of Biosafety Cabinets
Page 135 and 136: Minimizing Aerosols • Use careful
Page 137 and 138: Use Extreme Care with Sharps • Us
Page 139 and 140: Signs and Labels
Page 141 and 142: Biohazard Label • Must be attache
Page 143 and 144: It is important that the laboratory
Page 145 and 146: Moving the Biosafety Cabinet • Di
Page 147 and 148: Shipment and Transportation
Page 149 and 150: Emergency Response to Biological In
Page 151 and 152: Exposure Management For needlestick
Page 153 and 154: Biological Spill Clean-Up Kit- Basi
Page 155 and 156: Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158: Biosecurity
Page 159 and 160: Inspection
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Principles and Practices of Biosafety - San Diego State University

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