Principles and Practices of Biosafety - San Diego State University

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Bacterial Laboratory Acquired Infections • 76% of exposures occurred in clinical labs; 6% in vaccine manufacturing facilities; 8% in research labs. • Exposure modes: 60% inhalation, other exposure modes included ingestion (intentional, poor technique such as mouth pipetting, smoking and eating in the lab) and secondary transmission.
Viral Laboratory Acquired Infections • >70% associated with research labs; 32% of all viral LAIs associated with animals • 18% of total were Hantavirus; of these, 8% were working with known infectious material or rodents - others thought they were working with uninfected rodents • 16% were in clinical labs; rest were in production or field work • Major exposure modes: inhalation, percutaneous (especially from animals)
Page 1 and 2: Principles and Practices of Biosafe
Page 3 and 4: Introduction The management of biol
Page 5 and 6: SDSU Biosafety Requirements and Gui
Page 7 and 8: Institutional Biosafety Committee a
Page 9 and 10: Submission of New BUA • New infec
Page 11 and 12: Principal Investigator • Develops
Page 13 and 14: Risk Group and Biosafety Level
Page 15 and 16: Classification of Infective Agents
Page 17 and 18: Risk Group and Biosafety Level Clas
Page 19 and 20: Introduction • California Code of
Page 21 and 22: Exposure Determination • Exposure
Page 23 and 24: Hepatitis B Vaccination and Post-Ex
Page 25: Pathogenic Microbiology
Page 29 and 30: Molecular Biology
Page 31 and 32: Risk Assessment The following risk
Page 33 and 34: Donor Organism and Cloned DNA Inser
Page 35 and 36: Transgenic and “Knock-Out” Anim
Page 37 and 38: Human Source Material • Blood and
Page 39 and 40: Cell Culture Risks • Contaminatin
Page 41 and 42: Cell Culture Safety • Extend Univ
Page 43 and 44: Animals and Allergens
Page 45 and 46: Risks Associated with the Agent Use
Page 47 and 48: Transmission of Biohazards During W
Page 49 and 50: Transmission of Biohazards During W
Page 51 and 52: Risk Reduction: Containment of Infe
Page 53 and 54: Containment Caging Systems • Micr
Page 55 and 56: Containment Caging Systems • Can
Page 57 and 58: Special Animal Housing Situations
Page 59 and 60: Some Animals and Their Zoonoses •
Page 61 and 62: Transmission of Zoonoses • Enteri
Page 63 and 64: Laboratory Acquired Allergies (LAA)
Page 65 and 66: Routes of Exposure to LAA • Inhal
Page 67 and 68: LAA: Exposure Control • Engineeri
Page 69 and 70: Why Disinfect • To reduce or elim
Page 71 and 72: Classes of Disinfectants • Chlori
Page 73 and 74: • Organic Load Blood, sputum, mil
Page 75 and 76: • Contact Time Disinfectants shou
Page 77 and 78:
Medical Waste Disposal (Biohazardou
Page 79 and 80:
Medical Waste …biohazardous waste
Page 81 and 82:
Medical Waste Does Not Include: •
Page 83 and 84:
Sharps Waste … means any device h
Page 85 and 86:
Biohazard Bag …means a disposable
Page 87 and 88:
• Containers Containers shall be
Page 89 and 90:
Containment and Storage Sharps Wast
Page 91 and 92:
Solid Medical Waste Collection Must
Page 93 and 94:
What’s Wrong with these Pictures
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Containment Equipment and Facilitie
Page 103 and 104:
Primary Barrier • Primary barrier
Page 105 and 106:
Primary Barriers - Equipment • Pe
Page 107 and 108:
Chemical Fume Hood • 100 fpm face
Page 109 and 110:
Clean Bench / Laminar Flow Hoods
Page 111 and 112:
Class I Cabinet • 75 fpm face vel
Page 113 and 114:
Any Comments BSC - remove unnecessa
Page 115 and 116:
Types of Biosafety Cabinets NSF/ANS
Page 117 and 118:
Other Primary Barriers- Engineering
Page 119 and 120:
Biosafety Practices and Procedures
Page 121 and 122:
Administrative Controls • Substit
Page 123 and 124:
Personal Protective Clothing and Eq
Page 125 and 126:
BSL 1: Work Practices and Procedure
Page 127 and 128:
BSL 2: Work Practices and Procedure
Page 129 and 130:
Correct Use of Biosafety Cabinets
Page 131 and 132:
Correct Use of Biosafety Cabinets
Page 133 and 134:
Safe Use of Centrifuge • Use seal
Page 135 and 136:
Minimizing Aerosols • Use careful
Page 137 and 138:
Use Extreme Care with Sharps • Us
Page 139 and 140:
Signs and Labels
Page 141 and 142:
Biohazard Label • Must be attache
Page 143 and 144:
It is important that the laboratory
Page 145 and 146:
Moving the Biosafety Cabinet • Di
Page 147 and 148:
Shipment and Transportation
Page 149 and 150:
Emergency Response to Biological In
Page 151 and 152:
Exposure Management For needlestick
Page 153 and 154:
Biological Spill Clean-Up Kit- Basi
Page 155 and 156:
Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158:
Biosecurity
Page 159 and 160:
Inspection
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