Principles and Practices of Biosafety - San Diego State University

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Abbreviations • BSC – Biosafety Cabinet • BSL – Biosafety Level • ABSL – Animal Biosafety B Level • BSO – Biosafety Officer • BUA – Biological Use Authorization • CA – California • CDC – Center for Disease Control and Prevention • DGR – Dangerous Goods Regulations • DOT – Department of Transportation • EHS – Environmental Health and Safety • EPA – Environmental Protection Agency • HMR – Hazardous Materials Regulations • IACUC – Institutional Animal Care and Use Committee • IATA – International Air Transport Association • IBC – Institutional Biosafety Committee • IRB – Institutional Review Board • NIH – National Institute of Health • OSHA – Occupational Health and Safety Administration • PI – Principal Investigator • PP – Physical Plant • PPE – Personal Protective Equipment • PS – Pubic Safety • SD – San Diego • USPS – U.S. Postal Service
Introduction The management of biological hazards through the proper application of engineered containment and administrative controls is referred to as biosafety or biohazard control. Biosafety or biohazard control is a team effort involving the PI, research lab personnel, BSO, IBC, IRB, IACUC, EHS, PP and PS.
Page 1: Principles and Practices of Biosafe
Page 5 and 6: SDSU Biosafety Requirements and Gui
Page 7 and 8: Institutional Biosafety Committee a
Page 9 and 10: Submission of New BUA • New infec
Page 11 and 12: Principal Investigator • Develops
Page 13 and 14: Risk Group and Biosafety Level
Page 15 and 16: Classification of Infective Agents
Page 17 and 18: Risk Group and Biosafety Level Clas
Page 19 and 20: Introduction • California Code of
Page 21 and 22: Exposure Determination • Exposure
Page 23 and 24: Hepatitis B Vaccination and Post-Ex
Page 25 and 26: Pathogenic Microbiology
Page 27 and 28: Viral Laboratory Acquired Infection
Page 29 and 30: Molecular Biology
Page 31 and 32: Risk Assessment The following risk
Page 33 and 34: Donor Organism and Cloned DNA Inser
Page 35 and 36: Transgenic and “Knock-Out” Anim
Page 37 and 38: Human Source Material • Blood and
Page 39 and 40: Cell Culture Risks • Contaminatin
Page 41 and 42: Cell Culture Safety • Extend Univ
Page 43 and 44: Animals and Allergens
Page 45 and 46: Risks Associated with the Agent Use
Page 47 and 48: Transmission of Biohazards During W
Page 49 and 50: Transmission of Biohazards During W
Page 51 and 52: Risk Reduction: Containment of Infe
Page 53 and 54:
Containment Caging Systems • Micr
Page 55 and 56:
Containment Caging Systems • Can
Page 57 and 58:
Special Animal Housing Situations
Page 59 and 60:
Some Animals and Their Zoonoses •
Page 61 and 62:
Transmission of Zoonoses • Enteri
Page 63 and 64:
Laboratory Acquired Allergies (LAA)
Page 65 and 66:
Routes of Exposure to LAA • Inhal
Page 67 and 68:
LAA: Exposure Control • Engineeri
Page 69 and 70:
Why Disinfect • To reduce or elim
Page 71 and 72:
Classes of Disinfectants • Chlori
Page 73 and 74:
• Organic Load Blood, sputum, mil
Page 75 and 76:
• Contact Time Disinfectants shou
Page 77 and 78:
Medical Waste Disposal (Biohazardou
Page 79 and 80:
Medical Waste …biohazardous waste
Page 81 and 82:
Medical Waste Does Not Include: •
Page 83 and 84:
Sharps Waste … means any device h
Page 85 and 86:
Biohazard Bag …means a disposable
Page 87 and 88:
• Containers Containers shall be
Page 89 and 90:
Containment and Storage Sharps Wast
Page 91 and 92:
Solid Medical Waste Collection Must
Page 93 and 94:
What’s Wrong with these Pictures
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Containment Equipment and Facilitie
Page 103 and 104:
Primary Barrier • Primary barrier
Page 105 and 106:
Primary Barriers - Equipment • Pe
Page 107 and 108:
Chemical Fume Hood • 100 fpm face
Page 109 and 110:
Clean Bench / Laminar Flow Hoods
Page 111 and 112:
Class I Cabinet • 75 fpm face vel
Page 113 and 114:
Any Comments BSC - remove unnecessa
Page 115 and 116:
Types of Biosafety Cabinets NSF/ANS
Page 117 and 118:
Other Primary Barriers- Engineering
Page 119 and 120:
Biosafety Practices and Procedures
Page 121 and 122:
Administrative Controls • Substit
Page 123 and 124:
Personal Protective Clothing and Eq
Page 125 and 126:
BSL 1: Work Practices and Procedure
Page 127 and 128:
BSL 2: Work Practices and Procedure
Page 129 and 130:
Correct Use of Biosafety Cabinets
Page 131 and 132:
Correct Use of Biosafety Cabinets
Page 133 and 134:
Safe Use of Centrifuge • Use seal
Page 135 and 136:
Minimizing Aerosols • Use careful
Page 137 and 138:
Use Extreme Care with Sharps • Us
Page 139 and 140:
Signs and Labels
Page 141 and 142:
Biohazard Label • Must be attache
Page 143 and 144:
It is important that the laboratory
Page 145 and 146:
Moving the Biosafety Cabinet • Di
Page 147 and 148:
Shipment and Transportation
Page 149 and 150:
Emergency Response to Biological In
Page 151 and 152:
Exposure Management For needlestick
Page 153 and 154:
Biological Spill Clean-Up Kit- Basi
Page 155 and 156:
Spill Clean-Up for BSL 1-2 If the s
Page 157 and 158:
Biosecurity
Page 159 and 160:
Inspection
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