Principles and Practices of Biosafety - San Diego State University

Spill Clean-Up for BSL 1-2
If the spill is in the laboratory but outside the biosafety cabine
• Call the BSO if the material is RG 2 or greater.
• Clear area of all personnel. Wait at least 30 minutes for aerosol ol to settle
before entering spill area.
• Remove any contaminated clothing and place in biohazard bag to be b
autoclaved.
• Put on disposable gown, safety glasses and gloves.
• Initiate cleanup with disinfectant as follows:
Place dry paper towels on spill then layer a second set of disinfectant
soaked paper towels over the spill.
Encircle the spill with additional disinfectant being careful to minimize
aerosolization while assuring adequate contact.
Allow at least a minimum of 20 minutes contact time to ensure germicid
rmicid
action of disinfectant. Wipe up spill, working from the edges to the
center. Clean spill areas with fresh paper towels soaked in disinfectant.
infectant.
Decontaminate all items within the spill area.
Discard contaminated disposable materials using appropriate
biohazardous waste disposal procedures.