Principles and Practices of Biosafety - San Diego State University

Factors Influencing Efficacy
• Surface/Topography – uneven, cracked or pitted
surfaces especially wooden surfaces can hide
microorganisms and are difficult to disinfect
• Temperature - elevated temperatures may
enhance germicidal action but also evaporation
rate
• Relative Humidity – many disinfectants have
optimal relative humidity range for maximum
effectiveness
• Water Hardness – some disinfectants may be less
effective when diluted in hard water