Ph.D Programs in Agricultural Microbiology

manipulative enzymes - Polymerase Chain Reaction (PCR) - different
versions of PCR- application of PCR methods
Unit III
Cloning - vectors based on p
construction of recombinant DNA molecules-Introduction of recombinant
DNA into bacterial cells- Transformation, transduction, conjugation,
electroporation.
Unit IV 
Const ruction of genomic library and cDNA library - Identification of
correct clone through primers, probes & antibodies - DNA sequencing –
Methods in Genome sequencing - expression of cloned genes in
homologous & heterologous hosts – 16S rRNA, molecular markers, GFP,
lacZ, GUS.
Expression in recipient cell – methods of scoring markers – DNA
hybridization, southern blotting, western – western blot techniques –
screening for DNA polymorphism – RAPD- VNTRs- SSRs- Gene tagging
and techniques for DNA finger printing.
7
– 
UnitV
Application in m
Practical
Isolation - ofbacterialgenom plasmid DNA ic DNA
from bacteria - Agarose gel electrophoresis of DNA - Restriction digestion
of DNA-Restriction mapping - Ligation of DNA molecules - Microbial DNA
amplification by PCR, random primers, specific primers - Transformation
of E.coli-screening of transformants - expression of cloned genes in E.coliplasmid
curing in beneficial microbes-bacterial conjugation- transposon
mutagenesis- lysogenic and lytic phage- phage DNA isolation.
Lecture Schedule
1. Microbial genetics - Genetic elements in microbes
2. Tools & techniques for molecular biology
3. Genetic and phenotypic variation
4. Molecular aspects of gene expression