Ph.D Programs in Agricultural Microbiology
Ph.D Programs in Agricultural Microbiology
Ph.D Programs in Agricultural Microbiology
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
7. Induction of mutation <strong>in</strong> microbes by physical agents<br />
8. Restriction digestion of plasmid and genomic DNA<br />
9. Size determ<strong>in</strong>ation of DNA molecules<br />
10. Restriction mapp<strong>in</strong>g<br />
11. Elution of DNA from Agarose gel<br />
12. Ligation of DNA molecules<br />
13. Preparation of E.coli competent cells<br />
14. Transformation of E.coli -lacZ selection of recomb<strong>in</strong>ant E.coli<br />
15. Expression of cloned gene <strong>in</strong> E. coli (SDS-PAGE of prote<strong>in</strong>s)<br />
16. Microbial DNA amplification by PCR<br />
17. Practical exam<strong>in</strong>ation<br />
Suggested read<strong>in</strong>gs<br />
Ausubel. F.M., R.Brent, R.E.K<strong>in</strong>gston, D.D.Moore, J.G.Seidman,<br />
J.A.Smith and K. Struhl. 1999. Short Protocols <strong>in</strong> Molecular Biology.<br />
Fourth Edition. John Wiley & Sons, Inc. New York.<br />
Gene Clon<strong>in</strong>g and DNA Analysis: An Introduction. Fourth Edition.<br />
Blackwell Science Inc., Oxford, UK.<br />
Brown.T.A.2001.<br />
Primrose. S., R. Twyman and B. Old. 2001. Pr<strong>in</strong>ciples of Gene<br />
Manipulation. Sixth Edition. Blackwell Science Limited, Oxford.<br />
J., E.F. Fritsch and T. Maniatis. 2000. Molecular Clon<strong>in</strong>g: A laboratory<br />
Manuel. Hird Edition. Cold Spr<strong>in</strong>g Harbor Press, New York.<br />
Sam brook,<br />
Streips, U.N. and R.E. Yasb<strong>in</strong>. 2006. Modern Microbial Genetics. Wiley –<br />
Liss. John Wiley & sons, Inc. Publication, NY.
Change language