Ph.D Programs in Agricultural Microbiology

Polymorphism (RFLP) and Random Amplified Polymorphic DNA
(RAPD) – Microsatellite Markers – Ribotyping - phylogenetic
backbone for prokaryotic systematics.
16
Unit V
Num – Adensonian ericaltaxonom method y of classificationpolyphasic
taxonomy – antigenic typing: ELISA and Radio immunoassayphage
typing – DNA probes and their application in Microbial Ecology –
culture collections as essential resources for Microbiology – patenting of
microbial cultures.
Practical
M l differentiation orphologicaland of eubaeteria cultura and
archaeobacteria, Gram +ve and Gram – ve bacteria - Biochemical
characterization of prokayayotes eubacteria and archaeobacteria, Gram
+ve and Gram –ve bacteria- intrinsic antibiotic suseptibitiy of prokaryotes
eubacteria and archaeobacteria; Gram +ve and Gram –ve bacteria;
Biochemical techniques for identification of bacteria; CFA, SDS –PAGE,
isozymes pattern and zymogram analysis-Molecular techniques for
identification of bacteria; G+C content, DNA-DNA hyhridization, DNA-RNA
hybridization RAPD, RFLP, dendrogram analysis, microsatellite markers
for strain identification – immunometric assay like ELISA /western blot,
radioimmunoassay for strain identification- Numerical taxonomy using
softwares.
Lecture Schedule
1. Evolution of earth and earliest life forms- primitive organisms:
2. Molecular coding and energy generation
3. Eukaryotes and organelles –origin of modern eukaryotes – evolutionary
chronometers-
4. Ribosomal RNA sequences and cellular evolution
5. General principles of classification-classification, nomenclature and