Ph.D Programs in Agricultural Microbiology

Outcome
microbial diversity in various natural environments including extreme
After com pleting
environments such as hyperthermal, hypersaline, and anoxic habitats
•Evaluate approaches to isolate, cultivate, and classify
prokaryotic microorganisms
•Use aerobic and anaerobic techniques to isolate and
cultivate prokaryotes from natural and 
 man-made environments
•Analyze DNA sequences to identify prokaryotes using
molecular databases and 
 phylogenetic software 
 2.3. AGM 608
Advanced Methods for Identification of Microorganisms (2 + 1)
Objective 
To understand basic conce
microorganisms in conjuction with 
 advancements in identifying
and classifying them.
To update students
tools in phylogeny and identification of microbes. 
 Unit I 
M olecular
coding and energy generation –origin of modern eukaryotesevolutionary
chronometers- Ribosomal RNA sequences and cellular
evolution. General principles of classification- - International Code
of Nomenclature of Bacteria-major developments in bacterial
classification- classification of prokaryotic organisms and concept of
bacterial speciation –Etymology in nomenclature of prokaryotes.
Unit II 
M orphological,
biochemical, metabolic, enzymic, reproductive and molecular
biological features for delineating prokaryotes from eukaryotes,
bacteria from archaea and Gram - ve bacteria from Gram+ve
bacteria- Isolation and characterization of anaerobes and
extrmophiles. 
 Unit III
Biochemical techniques: Instrumentation of GC/ Gs-MS - Cellular
fatty acid analysis (CFA), protein profile by SDS-PAGE, isozyme
pattern and zymogram analysis- Molecular taxonomy: GC content,
DNA / DNA hybridization, DNA/ RNA hybridization, 16S rRNA
sequence analysis –DNA finger printing. 
 Unit IV
Electrophoresis (PFGE), Restriction Fragment Length
-
M odern approaches to c
Pulsed Field G e