Ph.D Programs in Agricultural Microbiology

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manipulative enzymes - Polymerase Chain Reaction (PCR) - different versions of PCR- application of PCR methods Unit III  Cloning - vectors based on p construction of recombinant DNA molecules-Introduction of recombinant DNA into bacterial cells- Transformation, transduction, conjugation, electroporation. Unit IV  Const ruction of genomic library and cDNA library - Identification of correct clone through primers, probes & antibodies - DNA sequencing – Methods in Genome sequencing - expression of cloned genes in homologous & heterologous hosts – 16S rRNA, molecular markers, GFP, lacZ, GUS. Expression in recipient cell – methods of scoring markers – DNA hybridization, southern blotting, western – western blot techniques – screening for DNA polymorphism – RAPD- VNTRs- SSRs- Gene tagging and techniques for DNA finger printing. 7 –  UnitV Application in m Practical  Isolation - ofbacterialgenom plasmid DNA ic DNA from bacteria - Agarose gel electrophoresis of DNA - Restriction digestion of DNA-Restriction mapping - Ligation of DNA molecules - Microbial DNA amplification by PCR, random primers, specific primers - Transformation of E.coli-screening of transformants - expression of cloned genes in E.coliplasmid curing in beneficial microbes-bacterial conjugation- transposon mutagenesis- lysogenic and lytic phage- phage DNA isolation. Lecture Schedule 1. Microbial genetics - Genetic elements in microbes 2. Tools & techniques for molecular biology 3. Genetic and phenotypic variation 4. Molecular aspects of gene expression
5. lac operon and other operon models, fine structure 6. Types of plasmids and plasmid controlled characters 7. Conjugation and fertility factor in bacteria 8. Transposons mutagenesis 9. Bacterial transformation - theory and concept 10. Mapping of genes by transformation 11. Transduction - Generalized and specialized transduction 12. Mutation in microorganisms – spontaneous and induced 13. Mutagens – mechanism, mutation frequency & and reversion 14. Preparation of DNA from microbial cells 15. DNA manipulative enzymes 16. Plasmids as cloning vectors for E. coli 17. Mid semester examination 18. Bacteriophages as cloning vectors for E. coli 19. Cloning vectors for other microbes 20. Polymerase Chain Reaction 21. Construction of genomic library and cDNA Library 22. Direct and indirect selection of clones 23. DNA analysis for clone identification
Page 1 and 2: Academic program in Agricultural Mi
Page 3 and 4: fermentation. Unit V  Yeasttechno
Page 5 and 6: Peppler.H.J and D.Perlman.1979.Micr
Page 7 and 8: 24. Molecular basis of cell - cell
Page 9 and 10: 10. Regulation of lipid synthesis 1
Page 11 and 12: Unit IV  O ther topics ofcurrenti
Page 13: Suggested readings Annable, M.O., M
Page 17 and 18: 7. Induction of mutation in microbe
Page 19 and 20: metabolic profiling, genomics, prot
Page 21 and 22: 12. 17. 21. 10 29. Intercellular co
Page 23 and 24: viruses -Multiplication and disease
Page 25 and 26: 23. Isotypes, allotypes and idiotyp
Page 27 and 28: Unit IV  Phylogenetic - An Overvi
Page 29 and 30: 25. Bacteria - gram+ bacteria 26. B
Page 31 and 32: Outcome microbial diversity in vari
Page 33 and 34: identification- 6. International Co
Page 35 and 36: archeabacteria and Gram positive &

Ph.D Programs in Agricultural Microbiology

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