Effects of Dietary Phytoestrogens on Paternal Responsiveness and Maturation in the Biparental California Mouse Aaron T. Stamp, Trey Amador Table 1: Behavioral scores, in 15-min paternalbehavior tests, of male California mice fed highsoy, medium-soy, or lowsoy diets (N=16 per group). P-values are from Kruskal- Wallis tests comparing the three treatment groups. Description Median (range) P High Soy Medium Soy Low Soy In contact with pup a 1.5 (0, 30) 0.5 (0, 30) 2 (0, 31) 0.281 >1 Body length from pup a 0 (0, 9) 1 (0, 23) 0.5 (0, 9) 0.493 Jump b 0 (0, 70) 31 (0, 548) 22.5 (0, 462) 0.074 Attack pup b 0 (0, 1) 0 (0, 1) 0 (0, 1) 0.526 Eat c 0 (0, 0.019) 0 (0, 0.107) 0 (0 , 0.078) 0.203 Approach pup c 0.398 (0, 0.933) 0.298 (0, 0.982) 0.244 (0, 0.989) 0.922 Carry pup c 0 (0, 0) 0 (0, 0.005) 0 (0, 0) 0.368 Huddle pup c 0 (0, 0.783) 0 (0, 0.958) 0 (0, 0.924) 0.400 Lick pup c 0 (0, 0.811) 0 (0, 0.814) 0 (0, 0.967) 0.388 Sniff pup c 0.119 (0, 0.801) 0 (0, 0.505) 0.0924 (0, 0.985) 0.959 Latency to approach pup d 84.120 (0, 330.62) 74.840 (2.48, 503.81) 22.460 (0, 71.92) 0.456 a Number of 30-sec instantaneous scans b Total number of occurrences c Duration as proportion of total time d Seconds although the LS group had a higher average sperm count than the HS and MS groups. Body Mass A 2-way (diet x week) repeated-measures ANOVA, using mass at weaning, weekly mean masses for weeks 1-10 after weaning, and final body mass (day before euthanasia), revealed a significant main effect of week Table 2: Wet and dry weights of testes and adipose tissue from California mice fed high-soy, medium-soy, or low-soy diets (N=8 per group), as well as sperm counts per mouse (N=8 for LS and MS groups, N=7 for HS group). Organ Wet/Dry Mean ± SEM (g) P High Soy Medium Soy Low Soy Left testis Wet Dry 0.162 ± 0.025 0.024 ± 0.003 0.173 ± 0.027 0.026 ± 0.003 0.170 ± 0.013 0.028 ± 0.001 0.532 a 0.888 a Right testis Wet Dry 0.151 ± 0.023 0.024 ± 0.003 0.167 ± 0.023 0.027 ± 0.003 0.165 ± 0.014 0.028 ± 0.002 0.648 a 0.898 a Left gonadal fat pad Wet Dry 0.173 ± 0.021 0.126 ± 0.020 0.199 ± 0.232 0.109 ± 0.171 0.217 ± 0.035 0.141 ± 0.030 0.501 a 0.544 a Right gonadal fat pad Wet Dry 0.166 ± 0.023 0.116 ± 0.017 0.217 ± 0.027 0.116 ± 0.019 0.216 ± 0.036 0.160 ± 0.031 0.234 a 0.171 a Left longitudinal fat pad Wet Dry 0.784 ± 0.117 0.448 ± 0.089 0.805 ± 0.118 0.508 ± 0.102 0.853 ± 0.139 0.519 ± 0.105 0.758 a 0.590 a Right longitudinal fat pad Wet Dry 0.813 ± 0.126 0.442 ± 0.071 0.861 ± 0.117 0.536 ± 0.090 0.861 ± 0.143 0.524 ± 0.101 0.791 a 0.435 a Back fat pad Wet Dry 0.328 ± 0.188 0.051 ± 0.039 0.402 ± 0.084 0.159 ± 0.035 0.481 ± 0.117 0.267 ± 0.063 0.259 a 0.168 a Sperm Count (X 106) 12.89 ± 1.04 12.97 ± 3.07 13.72 ± 2.63 0.967 b a Main effect of diet from ANCOVA using body mass as a covariate. b Main effect of diet from one-way ANOVA. 4 6 U C R U n d e r g r a d u a t e R e s e a r c h J o u r n a l
Effects of Dietary Phytoestrogens on Paternal Responsiveness and Maturation in the Biparental California Mouse Aaron T. Stamp, Trey Amador (F[11,473]=520.414, P