A report on an experiment I did of doing electrophoresis with proteins

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IV.
CONCLUSION
This experiment and the associated analysis shows a careful analysis of the limitations of being able predict mass
of a protein in a given sample by looking at intensitities of dye stains. In the process the use of running a serial
dilution of the protein solution through an SDS-PAGE electrophoresis set-up is found to be helpful to find out the
dependence of the intensity of the stain on the mass. Analysis of that shows that though intensities of stain seem to
show not so regular behaviour with the mass of the protein the intensities show a power-law fall with masses of the
protein with exponents and pre-factors which seem robust across experiments. Hence as an end result one can get
standardization curves which can be used to either predict masses of proteins in a random solution if in one sample
the mass is exactly known or ratios of masses in different samples in any case.
Doing of the experiment also introduced me to new experimental skills involved in handling microliters of fluids
and doing image analysis.
V. ACKNOWLEDGEMENT
I would like to express my deep gratitude for Prof.Roop Mallick for letting me intern in his laboratory for these 2
months and for giving me immense freedom to explore various possibilities and suggesting crucial checks and cautions
while analyzing the results.
I was fortunate to receive and extraordinary amount of help from Pradeep. It was heartening to see him even
compromise on his examination peparations and his own research to help me with every experiment that I tried and
especially for patiently teaching me all the basic techniques. For every problem in the laboratory I found Pradeep’s
kind help to save the day.
Further I would like to thank Ashim for helping me through a Western Blotting experiment and also for his timely
and sharp insights with feasibility of experiments.
I would like to thank Ravi for letting me use some of the solutions of Actin protein which he along with the others
had painstakingly extracted and purified.
I would like to thank members of Prof.Ulhas Kolthur’s lab for letting me use their water heater and antibodies ever
so often. Once I also had to use the water bath in Prof.Shubha Tole’s lab. Thanks are also due to Prof. Gotam. K.
Jarori’s lab for letting me use their 30% Acrylamide solutions.
Lastly I would like to thank the Suject Board of Physics at TIFR for providing this enriching experience.
5 “Electrobloting of proteins from polyacrylamide gels” by Michael.J.Dunn, published in“Protein Purification Protocols” edited
by Paul Cutler
5 “Using Antibodies” by Ed Harlow and David Lane
5 “Disc Electrophoresis-I” by Leonard Ornstein
5 “Isotachophoresis-Some fundamental aspects” by Jozef Leonardus Beckers
5 Wikipedia