Zeiss LSM510 Meta Confocal Microscope User Guide

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Fluorescence light adjustment no ND filters Near the back of the scope on the right are adjusters: Field stop slide up and down Aperture stop in back Focusing Manual focus as usual To set the focal plane as Zero Press Zero button on right of scope The 2 other buttons are Work (top) and Load (bottom) When in focus, press and hold Work button to set Load will then drop nosepiece and Work will return to Work position Do not change focus in Load position or a new Work is set If nosepiece won’t move up, you have to delete the Work position Press top button and hold while manual focusing until in focus, then release and new Work is set To avoid confusion, don’t use these 2 buttons, just manually return to the Zero position using the output on the little screen Brightfield – Transmitted light Switch lamps with buttons on right near front of scope Halogen lamp rheostat in front of scope at bottom Set Kohler Illumination Adj condenser aperture for desired contrast motor on top left of condenser
<strong>Confocal</strong> Mode LSM icon to switch to confocal scan Changes lamps, light path Imaging Setup icon under the setup manager Single Track for simultaneous acquisition, click the pro button at the upper right corner of imaging setup window, you can see the single track configs on the bottom. Multi Track configs are on the top in Configuration, the button next to it is "Apply". You can assign colors to the channels here. If you want to see the brightfield/DIC channel, check Ch D under Setup Manger / Light Path / Acquisition If you want to manually set up the lasers / filters / mirrors / dichriocs, also go to Setup Manger / Light Path / Acquisition Mode Channel Mode for normal scanning For Transmitted Light must use NONE for filter below stage After adjusting, Store (button next to apply) with a new name You can minimize this window Acquisition Mode icon under Online Acquisition Frame size in pixels, 512 x 512 Speed, use 6-9 Bits, 8 bits = 256 grey levels, 12 bits = 4096 grey levels For Scan direction, click pro at the upper right corner Averaging Line or Frame (line is not faster) Mean (averages) or Sum (accumulates) Scan Area (at the bottom of the Acquisition Mode window) Zoom – (use crop tool on image window) Must Stop scanning. Under 1.0x you cannot rotate the scan Rotation, X- or Y- move To get rid of the Zoom/Rotation, Reset All Channels tab SET BEFORE SCAN for EACH channel every time you switch objectives: Pinhole = 1 Airy make all channels have = section thickness for Z-series or colocalization start from the largest section (longest wavelength, or red) Gain - start at ~500 Offset is OK Amplifer Gain - keep at 1 unless very low signal Laser (AOTF) keep optimal low %
Page 1: Zeiss 510 Meta w/ ZEN Confocal Micr
Page 5 and 6: Z-Stack Z-Stack icon under Multidim
Page 7 and 8: Zeiss Axiovert 200 Microscope Specs
Page 9: Set-up for First-time users of the

Zeiss LSM510 Meta Confocal Microscope User Guide

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