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Zeiss LSM510 Meta Confocal Microscope User Guide

Zeiss LSM510 Meta Confocal Microscope User Guide

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Set-up for First-time users of the <strong>Zeiss</strong> <strong>LSM510</strong> software<br />

(System #209385)<br />

Login to Windows with your Domain username and password<br />

Find the New <strong>User</strong> Initialization files (Usually on the D drive)<br />

Initialize_New_<strong>User</strong>_Singletracks<br />

Initialize_New_<strong>User</strong>_Multitracks<br />

Initialize_New_<strong>User</strong>_Macros (if desired)<br />

Initialize<br />

From the LSM 510 software:<br />

Click Options, Settings<br />

Program Start:<br />

Check “don’t show logo”<br />

Save:<br />

Check the third bullet - “At “Create Database” automatically<br />

create a subdirectory with same name as specified database<br />

and create dataset image files in that subdirectory.”<br />

Check “Save prompt at Closing Modified Windows” and “Warning<br />

before overwriting existing recordsets”<br />

Temporary Files:<br />

“Use RAM” is fine, unless you are doing time lapse, then you may<br />

want to set a temporary location, e.g. your server directory<br />

Recording/Reuse:<br />

UNcheck all<br />

Time series: Check “Time Interval”<br />

Image Status Display:<br />

Check all selections under “Status display in image window” and “Image<br />

status bar”<br />

Don’t check “Show status display upon opening of new image display”

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