Zeiss LSM510 Meta Confocal Microscope User Guide

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Focus, Stage and Tile Scan Focus - set / change focus (Z) with software Choose step size Up arrow moves nosepiece up Stage - move / change positions on stages in X-Y directions Finding and marking sites of interest Move to first position Zero – defines this position as 0,0 Mark position Set distance to move Arrows go in opposite direction from image on screen Or move to a new location and mark Go to zero Goes to each position and scans To combine with Time, see Multitime series Tile Scan (aka stitching) Move to first position - this is center (?) Choose #X and #Y – will move based on pixel resolution (eg 512 x 512) Moves up and to the right first (?) Before start, check Tile Scan under Multidimensional Acquisition / Information On Experiment Start to start tile scan Bleaching (under Multidimensional Acquisition) Draw ROIs with Regions (under Online Acquisition) Setup bleaching / FRAP experiment Before start, check Bleaching under Multidimensional Acquisition / Information On Experiment Start to start bleaching You could combine all or any of the Z-stack, Time series, Bleaching, and Tile Scan. You could always Reuse your imaging settings by open a previous .lsm file and click the "reuse" button under the image window. All settings are saved for reuse on a .lsm file except the objective, please match the objective properly before reuse your settings. Shutdown Lasers OFF Hg lamp OFF Copy files to removable disk File – Exit Shut down Windows Wait until fan for argon laser shuts off automatically then – Components OFF and System OFF
<strong>Zeiss</strong> Axiovert 200 <strong>Microscope</strong> Specs Filters: 1 blank = LSM??? 2 Blue LP FS 02 ex 365/100 (300-400) bs 395 em 420 LP 3 Green FS 10 ex 470/40 (450-490) bs 510 em 540/50 (515-565) 4 Red FS 15 ex 546/12 (534-558) bs 580 em 590 LP 5 DIC Objectives: Mag NA Desc coverslip WD (mm) 5x 0.15 C Plan-NEOFLUAR 0.17 13.6 10x 0.3 C Plan-NEOFLUAR 0.17 5.6 20x 0.8 Plan-APOCHROMAT 0.17 0.55 40x 1.3 oil EC Plan-NEOFLUAR 0.17 0.20 63x 1.4 oil Plan-APOCHROMAT 0.17 0.19 40x 1.2 W C-APOCHROMAT c slip adj 0.28 DIC for all except 5x and 10x Section thickness in um Wavelength Obj 488nm 561nm 633mn 5x/0.15 47.2 um 52.7 um 60.1 um 10x/0.3 11.7 13.1 14.9 20x/0.8 1.6 1.7 2.0 40x/1.3 0.9 1.0 1.1 63x/1.4 0.8 0.8 0.9 40x/1.2W 0.9 1.0 1.1 “Optimal” step size is ½ section thickness.
Page 1 and 2: Zeiss 510 Meta w/ ZEN Confocal Micr
Page 3 and 4: Confocal Mode LSM icon to switch to
Page 5: Z-Stack Z-Stack icon under Multidim
Page 9: Set-up for First-time users of the

Zeiss LSM510 Meta Confocal Microscope User Guide

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