LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates

More documents

Recommendations

Info

Replicates 93 3 3 3 33002502001501005002 50 0 5 0 00 75 00Amount / Amt. Std.Rep lic ate s 24M15 012 510 07550250.999900 0.999925 0.999950 0.999975 1.000000 1.000025 1.000050 1.000075AmountAmphetamines Experimental ConditionsMethamphetamine: MS/MS BreakdownMethamphetamine Calibration CurveChromatographic data of AmphetaminesCalibration Curve ReportFile: e:\norchem\amps\amphetaminesdata multiplier2.mthDetector: 1200 Mass Spec, Address: 42Calibration Curve ReportFile: e:\norchem\amps\amphetaminesdata multiplier2.mthDetector: 1200 Mass Spec, Address: 42MethamphetamineC urve Fit: Linear, Origin: Ignore, Weight: None Resp. Fact. R SD : 5.318%y = +0.031914x +0.257271Coeff. Det.(r2): 0.997419Methamphetamine d5C urve Fit: Linear, Origin: Include (Ignore), W eight: N one Resp. Fact. R SD : 0.0000%y = +1.000000x +0.0C o eff. D et.( r2) : 1.00000 0Peak Size / PS Std.Peak SizeMethamphetamine 5-10,000 ng/mLMethamphetamine d5 ISSpiked: 50 ng/mL in urine
Amphetamine Summary Excellent sensitivity at the level of 5ng/mL Good separation from a column packed with afluorinated phenyl phase Detection limits will be at least the low ppb levels for allcompounds All 6 drugs can be run simultaneously in a 6 min. run Good linearity of calibration curves ranging from 5-10,000 ng/mLAnalysis of Carboxy-THCbyLC/MS/MSCarboxy -THC Experimental ConditionsChromatograms – Carboxy - THC ESI Negative. LC Conditions: Column: Varian Pursuit Diphenyl50X2mm, 3μ Flow Rate: 0.2 mL/min A: Water B: Methanol Time %B 0:00 40 0:30 40 1:00 95 3:00 95 3:01 40 6:30 40 Injection Volume, 20 μL Mass Spec conditions: Argon pressure: 2.0 mtorr Q1/Q3 peak width: 1.0/1.0amu Transitions: 343.50 -> 299 CE 17.5 352.50 -> 308 CE 20.5 SamplesIn a complicated matrixsolutionStandard Solutions preparedby serial dilutions in DI water50 μL IS added to 1 mL ofsampleCarboxy-THC – 10 ng/mLCarboxy-THC – 1 ng/mL
Page 1 and 2: Presentation OutlineLC/MS/MS Analys
Page 3 and 4: MSMS Using a Curved Collision CellI
Page 5 and 6: Analysis of Gabapentin byLC/MS/MSWh
Page 7 and 8: Introduction to BenzodiazepinesAnal
Page 9 and 10: 320-MS LC SystemsBreakdown CurvesHy
Page 11: Opiate resultsCustom ReportsBenzodi

LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?