LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates

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Representative Calibration Curves (10-1000ng/mL)Chromatogramof Benzo Mix,250 ng/mLCalibratorMCounts271.0>140.0 [-24.0V]100MCounts285.0>193.0 [-24.0V]500MCounts286.0>222.0 [-18.5V]500MCounts287.0>241.0 [-10.5V]0.0MCounts290.0>226.0 [-18.5V]20MCounts290.1>198.0 [-24.0V]200MCounts292.0>246.0 [-10.5V]40MCounts301.0>255.0 [-18.5V]200MCounts309.0>281.0 [-23.5V]400MCounts314.1>286.0 [-18.5V]0MCounts316.0>270.0 [-20.5V]40MCounts321.0>275.0 [-18.0V]4NordiazepamDiazepam7-AminoclonazepamOxazepamD-4 7-AminoclonazepamD-5 DiazepamD-5 OxazepamTemazepamAlprazolamD-5 AlprazolamClonazepamLorazepam7-AminoclonazepamWeight: None Curve Fit: Linear, Origin: Include, Resp. Fact. RSD: 8.179%y = +1.058993x -0.006779Coeff. Det.(r2): 0.9993Replicates32 2 2 2 2 25432Peak Size / PS Std.101 2 3 4 5Amount / Amt. Std. (ng/mL)ClonazepamInclude, Weight: None Curve Fit: Linear, Origin: Resp. Fact. RSD: 5.639%y = +1.054153x +0.013111Coeff. Det.(r2): 0.9978Replicates32 2 2 2 2 25432Peak Size / PS Std.1R(2) = 0.999RSD = 8.17R(2) = 0.997RSD = 5.6402.5 5.0 7.5 10.0 12.5 minutesSeg 1, Time: 0.05-15.10, Scan Functions: 282031 4107 6182 8257 10333 Scans01 2 3 4 5Amount / Amt. Std. (ng/mL)Submitted Samples Extracted from Plasma matrixAlternative Benzodiazepine and Method OpiatesUsingUsing the the 320MS Vial 01 Opiate Calibrator (500ng/mL) Codeine Hydrocodone Hydromorphone Morphine Oxycodone Vail 02 Unknown Vial 03 Opiate Internal Stds. (2500ng/mL) Codeine D3 Hydrocodone D6 Hydromorphone D6 Morphine D3 Oxycodone D6 Vial 4 Benzo Internal Stds. (250ng/mL) Nordiazepam D5 Alprazolam D5 Hydroxyalprazolam D5 Vial 5 Benzo Calibrator (250 ng/mL) Nordiazepam D5 Alprazolam D5 Hydroxyalprazolam Nordiazepam Alprazolam Hydroxyalprazolam D5 Flurazepam Oxyazepam Lorazepam Clonazepam Desalkylflurazepam Temazepam Diazepam Vials 6 and 7 Unknowns
320-MS LC SystemsBreakdown CurvesHydroxyalprazolamHydrocodone320-MS212-LCProStar 430320-MS212-LCHTS PALBenzodiazepines MethodBenzo Three Ion Monitoring Mobile Phase: 0.1% Formicaq/MeOH Nebulizer Gas: N 2 @ 30psi Drying gas: N 2 @ 24psi (350C) Flow rate: 200 uL/min Column: Pursuit XRS Diphenyl 100x 4.6mm 3umExample:Hydroxyalprazolam325>297 (Quant. Ion)325>216 (Qualifier)325>279 (Qualifier)
Page 1 and 2: Presentation OutlineLC/MS/MS Analys
Page 3 and 4: MSMS Using a Curved Collision CellI
Page 5 and 6: Analysis of Gabapentin byLC/MS/MSWh
Page 7: Introduction to BenzodiazepinesAnal
Page 11 and 12: Opiate resultsCustom ReportsBenzodi
Page 13 and 14: Amphetamine Summary Excellent sensi

LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates

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