LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates
LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates
LC/MS/MS Analysis of Gabapentin, Benzodiazepines, and Opiates
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Conditions <strong>MS</strong> Conditions:Ionisation Mode: ESI positiveScan Time: 1.0 sDrying Gas: 350 ˚C at 25 psiCollision Cell Pressure: 2.0 mTorrCapillaryVoltage PrecursorProductIonsCollisionEnergyCompounds (V) Ion (m/z) (m/z) (V)<strong>Gabapentin</strong> 65 172 154 -26.50<strong>Gabapentin</strong> 65 172 137 -29.00Bacl<strong>of</strong>en(IS) 65 214 151 -36.50<strong>Gabapentin</strong> <strong>MS</strong> Parameters HP<strong>LC</strong> Conditions: Column: Pursuit Diphenyl 50mm x2.0mm 3u Guard Column: Pursuit Diphenyl2.0mm I.D., 3u Solvent A: Water Solvent B: Methanol Flow Rate: 200µL/min Injection Volume: 25µL <strong>LC</strong> Program:%ATime (min)%B 0:00 60 40 1:001:306054095 3:30 5 95 3:35 60 40 5:00 60 40kCounts 1.0 ug ml std.xms172.0>137.01.0 ug ml std.xms500 172.0>154.040030020010001.0 ug/ml extracted <strong>Gabapentin</strong> (left); Bacl<strong>of</strong>en – IS(right)0.5 1.0 1.5 2.0 2.5minutesMCounts1.000.750.500.250.00BACLOFEN (IS) 1.0 ug mlstd.xms 151.0(214.0>151.0)0.5 1.0 1.5 2.0 2.5minutes<strong>Gabapentin</strong> Calibration Curve (1.0 ug/ml – 25 ug/ml)<strong>Gabapentin</strong> ConclusionG A B A P E N T I NC u r v e F i t : L i n e a r , O r i g i n : F o r c e , W e i g h t : N o n e R e s p . F a c t . R S D : 6 . 9y = + 1 . 0 7 0 8 0 6 xC o e f f . D e t . ( r 2 ) : 0 . 9R e p l i c a t e s1 1 1 1 1 12 . 52 . 01 . 51 . 0Peak Size / PS Std.0 . 5<strong>LC</strong>/<strong>MS</strong>/<strong>MS</strong> is utilized in the analysis <strong>of</strong> gabapentin because itallows for a simple, cost-effective clean up, requires noderivatization <strong>of</strong> the drug <strong>and</strong> provides accurate results withexcellent sensitivity.This application demonstrates that the Varian triple quadrupole<strong>LC</strong>/<strong>MS</strong>/<strong>MS</strong> can identify <strong>and</strong> quantify <strong>Gabapentin</strong> in postmortemspecimens with the use <strong>of</strong> two MRM transitions.Varian Inc. would like to provide a special thank you to SaraKegler <strong>and</strong> Dan Anderson from Los Angeles County Department<strong>of</strong> Coroner for the validation <strong>of</strong> both the extraction procedure<strong>and</strong> the instrument parameters.0 . 00 . 5 1 . 0 1 . 5 2 . 0 2 . 5A m t / M L A m o u n t / . S t d . ( U G )RSD = 6.9%; r2 = 0.999
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