An attempt at an epidemiological explanation - Epib.nl
An attempt at an epidemiological explanation - Epib.nl
An attempt at an epidemiological explanation - Epib.nl
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LOS immunotyping of N. meningitidis by weEunder the assumption th<strong>at</strong> the combin<strong>at</strong>ions L2,B <strong>an</strong>d LA,B do not exist (see Discussion),in which case LB is assigned. The assignment of immunotypes Ll, LS, L6<strong>an</strong>d LlD is based on a positive reaction with the specific moabs described above. AllLlD isol<strong>at</strong>es also reacted with Mn15A17F12. Combin<strong>at</strong>ions of immunotypes, such asLl,B, L3,l, L3,l,B <strong>an</strong>d L3,B, are also discernible. A positive reaction with the combin<strong>at</strong>ionMnI5AB-l (with or without M<strong>nl</strong>5A17F12 or 9-2-L3,7,9) on the oue h<strong>an</strong>d, <strong>an</strong>dMn13H21, 2-1-LB or Mn4DIB3 on the other, is considered non-interpretable in theabsence of a positive reaction with one of the other moabs. Isol<strong>at</strong>es th<strong>at</strong> failed toreact with <strong>an</strong>y moab were labelled nontypeable (NT).lmmunotypes <strong>an</strong>d moab reaction p<strong>at</strong>ternsTable 3 shows examples of the reaction p<strong>at</strong>terns of isol<strong>at</strong>es of the various immunotypes<strong>an</strong>d immunotype combin<strong>at</strong>ions. For the ITs L2 through L6, isol<strong>at</strong>es with aupurell reaction p<strong>at</strong>tern were found. However, all Ll <strong>an</strong>d LIO <strong>an</strong>d m<strong>an</strong>y 13 strainsshowed some reaction with the LB group of moabs (Mn13H21 <strong>an</strong>d 2-1-LB), <strong>an</strong>d m<strong>an</strong>yL8 isol<strong>at</strong>es with the L3 group (Mn4DIB3, MnI5A17FI2, 9-2-L3,7,9, MnI5AB-l),indic<strong>at</strong>ing th<strong>at</strong> the isol<strong>at</strong>es under consider<strong>at</strong>ion partly express other ITs.Tnble 3. Examples of reaction p<strong>at</strong>terns of isol<strong>at</strong>es of various immunotypcs <strong>an</strong>d immunotype combin<strong>at</strong>ionsto a p<strong>an</strong>el of 14 monoclonal <strong>an</strong>tibodies in a whole-cell EUSAMonoclonal <strong>an</strong>tibodylmmunotypc M.ll4P2o..U 14-1-L10 Mn15A17F12 Mn15AB- l Mn4A882 Mn4ClBStrain Mn14F21-11 Mn13H21 M<strong>nl</strong>lAllG 9-2-L3,7,9 Mn4D1B3 Mn3A8CID <strong>an</strong>d/or 17-1-U <strong>an</strong>d/or 2-1-L.8892633 1 + w +900997 1,8 + + +901005 1,8 + + + w + +891357 2 + + +892385 3 + + + + + +900755 3 w + + + + +900820 3 + + + + + + +892463 3,1 + + + + + +891 177 3,1 + w + + + + +900747 3,1,8 + + + + + + + +891021 3,8 + + + + + + +892214 4 +M981 5 w + +M992 6 +900714 8 + + w w891165 8 + + + + + w w900018 8,10 + + + +900100 10 w + w +892411 10,11 + + + + +7889 11 + + +NOrE: + = positive reaction; w = weak positive reaction51
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