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Division of Biosystems ResearchPlant Systems EngineeringResearch CenterRESEARCHERSSuk Yoon Kwon sykwon@kribb.re.krChol Gu Hur hurlee@kribb.re.krHye Ran Kim kimhr@kribb.re.krComparative, functional and structural genomics ofmajor cropsGenomics for molecular breeding of Brassica cropsEvolutionary genomicsJang Ryol Liu jrliu@kribb.re.krWon Joong Jeong wonjoong@kribb.re.krSung Ran Min srmin@kribb.re.krDevelopment of "CyanoCrops" by introducingcyanobacterial genes into the chloroplast genome ofcropsCloning of salt resistant genes from marinecyanobacteria via a functional genomics approach andthe development of salt-tolerant cropsMicroalgae genetics for biofuelsJae Heung Jeon jeonjh@kribb.re.krHyouk Joung joungh@kribb.re.krHyun Soon Kim hyuns@kribb.re.krMass production of the seeds or seedling of usefulvegetative-propagation cropsDevelopment of useful transgenic cropsEstablishment of the optimal system (eg, glycosyationpattern, RNAi knockdown) for molecular farmingHye Sun Cho hscho@kribb.re.krPhotosynthesis regulation and mechanismFunctions of chloroplast immunophilinsIdentification of rice immunophilinsJeong Mee Park jmpark@kribb.re.krMolecular mechanisms of pathogen - induced cell deathPlant immunity to virusesJae Sun Moon jsmoon@kribb.re.krMolecular plant-microbe interactionsDevelopment of the pathogen diagnosis oligo chipsIdentification of the genes involved in the developmentby virus-induced gene silencingRESEARCH AREASPlant genome structural, funtional and evolutionarygenomicsFunctional genomics of plant-microbe interactionsDevelopment of an environmentally-friendly binaryvector systemSignal transduction network of plant cell deathCyanoCrop using cyanobacterial genesOur center focuses on the development of green technologies and platform technologies to improve important plants. Theseinclude identifying functionally important genes as well as establishing a novel transformation system and new transgenicplants with useful traits. We have established platform technologies for structural and functional genomics of important cropsby conducting international Solanaceae genome project. We urrently enlarge our genomics platform technology for improvinguseful crops and developing breeding tools.Director Suk Yoon KwonT + 82-42-860-4300F + 82-42-860-4349E sykwon@kribb.re.krACHIEVEMENTSInternational collaboration on the analysis of the Solanaceae genomeLaunched in 2004, this ten-year project involves twenty nations, ten of which areparticipating in the primary operations to decode the genomic sequence. Korea isresponsible for the 2nd chromosome, which consists of 12% of the entire genome. So far,we have accomplished about 90% of our designated task.Large-scale isolation of pepper genes and public releaseWe have undertaken the task of gene identification in peppers, the most importantvegetable crop in Korea, and have finished analyzing about 120,000 expressed sequencetags. A database containing this information has been built and opened to the public. Webelieve that we have secured more than two-thirds of the pepper genome, which consistsof 30,000 uni-genes, and expect information of varieties to be helpful in the development ofnew strains. (http://sol.pdrc.re.kr)Development of platform technology for research on plant functional genomicsVirus-induced gene silencing technology, developed for the large-scale screening of genesis currently being used in the screening of many types of Solanaceae plants includingNicotiana benthamiana, peppers, and tomatoes.Development of transplastomic technologyWe have succeeded in developing transformation technology for foreign gene expressionin plastids. These genes can only be inherited from the maternal line, which cannot bespread through pollen, thereby ensuring a low environmental risk.Development of an edible plant-derived vaccine for Alzheimer's diseaseAntigens for mutant b-amyloid proteins, which are known to be a cause of Alzheimer'sdisease, were overexpressed in potatoes as an edible vaccine. We have developed thetransgenic potato, and have confirmed through experiments with mice that the potatovaccine does produce specific antibodies for b-amyloids.Development of genomics assisted breeding toolsWe have embarked on an ambitious genomics program entitled the 'Cabbage genomicsassisted breeding support project'. The long-term objective of the project is to create agenome-level closed breeding system for the Brassica oleracea that can be used as aresearch platform to study evolution, development, genome organization, polyploidy,domestication, gene regulatory networks and crop improvement.SELECTED PUBLICATIONSSuk Yoon Kwon (Corresponding) Biochem Biophys Res Commun. 399(4):750-4.Silencing of SlFTR-c, the catalytic subunit of ferredoxin:thioredoxin reductase, inducespathogenesis-related genes and pathogen resistance in tomato plantsHye Sun Cho (Co-corresponding) BMC Plant Biol. 10:253.Classification of rice (Oryza sativa L. Japonica nipponbare) immunophilins (FKBPs, CYPs)and expression patterns under water stressHyun Soon Kim (Corresponding) Clin Vaccine Immunol. 17(12):2029-32.Antibody responses in mice stimulated by various doses of the potato-derived majorsurface antigen of hepatitis B virusSuk Yoon Kwon (Corresponding) J Exp Bot. 61(9):2499-506.Tobacco seeds simultaneously over-expressing Cu/Zn-superoxide dismutase andascorbate peroxidase display enhanced seed longevity and germination rates under stressconditionsWon Joong Chung (Corresponding) Plant Cell Rep. 29(9):967-75.Cucumber mosaic virus 2b protein inhibits RNA silencing pathways in green algaChlamydomonas reinhardtiiWon Joong Chung & Hyun Soon Kim (Co-corresponding) Transgenic Res. 19(6):1099-108.High-level expression of a human β-site APP cleaving enzyme in transgenic tobaccochloroplasts and its immunogenicity in mice40 KRIBB Annual Report 2010KRIBB Annual Report 2010 41