Untitled

Jeonbuk Branch InstituteMicrobe-based FusionTechnology Research CenterRESEARCHERSJae Jun Song jjsong@kribb.re.krDevelopment of platform technologies for massivescreening and the commercialization of industriallyvaluable enzymes using the HTS systemDevelopment of the technology to prepare a genomiclibrary from single unculturable microorganisms sortedfrom natureChul Ho Kim kim3641@kribb.re.krBioprocessBio-refineryJoong Su Kim joongsu@kribb.re.krDevelopment of the glycosyltransferases useful for themanufacturing of glycodrugs and the glycosylation ofnatural compoundsDevelopment of the aldolases applicable to the whitebiotechnologyDevelopment of protocol to screen industrial enzymesfrom metagenomeJeong-Woo Seo jwseo@kribb.re.krMetabolic pathway engineering in microorganismsJong Hyun Choi jhchoi@kribb.re.krDevelopment of platform technologies for screeninguseful enzymes using High Thoughput Screening SystemDevelopment of the tool box applicable to the whitebiotechnology based on systems biotechnologyRESEARCH AREASMicrobial metabolic engineeringProduction of microbial metabolitesMetabolic engineering of industrial microorganismsBioconversion technologyHigh throughput screening of novel biocatalystsDirected evolution of industrial enzymesProduction of useful biomaterials by bioconversionMolecular bioprocess engineeringProduction of therapeutic recombinant proteinsDevelopment of bio-refinery technologiesDirector Jae Jun SongT + 82-63-570-5140F + 82-63-570-5109E jjsong@kribb.re.krACHIEVEMENTSDevelopment of 1,3-propanediol producing microbial strainsRecombinant strains optimized to produce 1,3-propanediol using crude glycerol, derivedfrom biodiesel industry by-products, were developed through metabolic pathwayengineering. Notably, the occurrence of by-products, which are usually an obstacle in thepurification process of 1,3-propandiol, was completely abolished in the engineered strains,resulting in conversion yields with the highest levels reported to date.Development of human papillomavirus vaccineSeveral types of human papillomavirus L1 genes encoding the major capsid protein wereexpressed in Escherichia coli. The structural proteins were found to be immuno-active bywestern blotting analysis and synthesized the virus-like particles by self-assembly in theheterologous host.Screening of the novel enzymesNovel 2-deoxyribosephosphate aldolases, glycosyltransferases, cellulases, lipases, andproteases were screened and isolated from nature, and cloned into E. coli.Preparation of a genomic library from a single microorganismMultiple Displacement Amplification technology was applied to the amplification ofgenomic DNA isolated from single cell, from which novel cellulase was selected.SELECTED PUBLICATIONSChul Ho Kim (Corresponding) Appl Microbiol Biotechnol. 85(3):659-66.Identification and utilization of a 1,3-propanediol oxidoreductase isoenzyme for productionof 1,3-propanediol from glycerol in Klebsiella pneumoniaeJeong-Woo Seo (Corresponding) Biotechnol Lett. 32(8):1077-82.Enhanced production of ethanol from glycerol by engineered Hansenula polymorphaexpressing pyruvate decarboxylase and aldehyde dehydrogenase genes from ZymomonasmobilisJae Jun Song (Corresponding) Int J Syst Evol Microbiol. 60(3):615-9.Jeongeupia naejangsanensis gen. nov., sp. nov., a cellulose-degrading bacterium isolatedfrom forest soil from Naejang Mountain in KoreaChul Ho Kim (Corresponding) J Ind Microbiol Biotechnol. 37(2):195-204.Gene cloning, characterization, and heterologous expression of levansucrase fromBacillus amyloliquefaciensJoong Su Kim (Corresponding) Microbiol Res. 165(5):384-91.Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001Our goal is to develop biotechnologies and bioprocesses for the production of microbial metabolites, proteins, industrial enzymesand bioenergy, all of which are useful for the phamaceutic, nutraceutic, dietetic, cosmetic, feed, fine chemical and other industries.76 KRIBB Annual Report 2010KRIBB Annual Report 2010 77