Division of Biosystems ResearchIndustrial Bio-materialsResearch CenterRESEARCHERSTae-Sook Jeong tsjeong@kribb.re.krSearch, in vivo efficacy test, mechanism study, anddevelopment of bioactive materials for prevention andtreatment of metabolic syndroms (including obesity,hyperlipidemia, atherosclerosis)Young Ik Lee yilee@kribb.re.krMolecular Biology, HepathologyDevelopment of antiviral, anti-cirrhosis and anti-livercancer agents using recombinant DNA techniques andidentifying active compounds from natural resourcesSung Uk Kim kimsu@kribb.re.krSearch and development of bio-materials for agricultureincluding biological control agents, fungicides, and TTSSinhibitors from natural resourcesHo-Yong Park hypark@kribb.re.krHighly active enzymes and bio-materials frominvertebrates & microbes for industrial applicationDevelopment of bio-insecticides for the control ofagricultural insects by insect pathogensKwang-Hee Son sonkh@kribb.re.krMicrobial BiotechnologyEnzymes for industrial and bio-energy applicationsMicrobial natural products for pharmaceutical usesHyun-Woo Oh hwoh@kribb.re.krSearch and development of bio-materials for agricultureincluding biological control agents, insecticides andinsect repellents from natural resourcesProvide imaging and analytical services, includingscanning and transmission EM for bio-researchRESEARCH AREASDevelopment of biodiversity-based bio-materialsthrough convergence technologiesDevelopment of highly active industrial enzymes frominsects and microbesDevelopment of biological catalysts to solve the biomassrecalcitrance for bio-fuelsFunctional study of bio-active substances from insectsand microbesDevelopment of antiviral agents for the treatment ofhepatitis BThree different antiviral agents were isolated fromnatural domestic plants.These antiviral agents which function differently withinthe HBV life cycle prevent the appearance of the drugresistantmutations, which commonly arise duringantiviral agent treatment.Development of a platform technology for metabolicsyndromeInvestigation into the cause of and the discovery oftargets for metabolic syndromeScreening of bioactive substances for the prevention andtreatment of metabolic syndrome and using thesesubtances for the development of functional foods andnutraceuticalsDevelopment of bio-materials inhibiting microbialfunctions from natural resourcesSearch and development of inhibitors for type IIIsecretion system responsible for the virulence ofphytopathogenic bacteriaSearch and development of novel bio-materials, fromnatural resources, exhibiting biological control effectsagainst phytopathogenic fungiResearch at the Industrial Bio-materials Research Center focuses on the basic and applied studies for the development highlyactive enzymes for industry, such as potent functional bio-materials for functional foods and therapeutic agents, and biologicalcontrol agents for agriculture. Our objectives are to discover and develop bio-materials from natural resources and make themavailable to industry, which increases quality-of-life and public welfare.Director Tae-Sook JeongT + 82-42-860-4558F + 82-42-861-2675E tsjeong@kribb.re.krACHIEVEMENTSDevelopment of a proteinase, ArazymeAn enzyme that degrades proteins, even under harsh conditions, was developed througha biodiversity-oriented screening system. Through translational research,the enzyme was customized to become an integral part of therapeutics, bio-functionalcosmetics, feed additives, detergents, waste-treatment and leather processing. Presently,arazyme-related products are being introduced to the global market by a spin-offcompany.Development of highly active xylanase and lipase from insects and microbesHighly active xylanase and lipase were developed from insects and microbes. Thesetechnologies were also transferred to a related corporation for industrialization.Development of anti-obesity, anti-atherogenic, and anti-diabetic agents for theprevention and treatment of metabolic syndrome from soy leavesThe pterocarpan compounds from soy (Glycine max) leaves showed the inhibitory activitiesof adipogenesis, LDL-oxidation, hACAT, α-glucosidase. In particular, the ethanolicextracts of soy leaves containing pterocarpans reduced body weights and fat tissues in 45Kcal% fat diet-fed C57BL/6J mice and suppressed arotic lesion formations in highcholesterol-fed LDLr deficient mice. These active materials can be utilized for thedevelopment of nutraceuticals and new drugs for the prevention and treatment ofmetabolic symdrome.Development of bio-materials inhibiting liver diseasesEstablishment of screening systems for the isolation of the bio-materials treating liverdiseases (improving liver functions, hepatitis B virus diseases, liver cirrhosis, liver cancer)Isolation and structural determination of biomaterials for curing liver diseasesEvaluation of efficacy in animal models and in human subjectsDevelopment of bio-materials exhibiting potent anti-fungal effectsagainst phytopathogeic fungiThe dimeric sesquiterpene CHE-23C isolated from Chloranthus henryi exhibited 91 and100% disease-control activity in vivo against tomato late blight and wheat leaf rust atconcentrations of 33 and 100 µg/ml, respectively. The disease-control activity of thiscompound was stronger than that of the commercially available fungicide chlorothalonil,but weaker than that of dimethomorph. Therefore, this compound might serve as aninteresting link in developing effective fungicides.SELECTED PUBLICATIONSTae-Sook Jeong (Corresponding) Biol Pharm Bull. 33(6):1019-23.Lavandulyl flavonoids from Sophora flavescens suppress lipopolysaccharide-inducedactivation of nuclear factor-kappaB and mitogen-activated protein kinases in RAW264.7cellsSung Uk Kim (Corresponding) Bioorg Med Chem Lett. 20(22):6551-4.Synthesis and antifungal activity of a novel series of 13-(4-isopropylbenzyl)berberinederivativesKwang-Hee Son & Ho Yong Park (Co-corresponding) Bioresour Technol. 101(22):8814-21.Novel intracellular GH10 xylanase from Cohnella laeviribosi HY-21: biocatalytic propertiesand alterations of substrate specificities by site-directed mutagenesis of Trp residuesSung Uk Kim (Corresponding) J Agric Food Chem. 58(24):12657-63.Qualitative and quantitative detection of agricultural microorganisms expressing iturin andmop cyclase in soilsKwang-Hee Son & Ho-Yong Park (Co-corresponding) J Mol Catal B. 62(1):32-9.Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydusHY-15 isolated from the gut of Eisenia fetida42 KRIBB Annual Report 2010KRIBB Annual Report 2010 43
Division of Biosystems ResearchEnvironmental BiotechnologyResearch CenterRESEARCHERSHee-Mock Oh heemock@kribb.re.krEcophysiological study of microalgae and biological CO2fixation using microalgaeSang-Soo Kwak sskwak@kribb.re.krTransgenic plants with enhanced tolerance to multiplestresses on marginal landsHaeng-Soon Lee hslee@kribb.re.krMolecular breeding of crops for bioenergy and functionalfeed on marginal landsStephen Beungtae Ryu sbryu@kribb.re.krEnhancement of multi-resistances of plants usingnatural lipids and plant green biotechnology on naturalrubberWon-Gon Kim wgkim@kribb.re.krCharacterization of bioactive substances frommicroorganisms and environmental sourcesHee-Sik Kim hkim@kribb.re.krMolecular analysis of microbial diversity and functions incontaminated environmentsChi-Yong Ahn cyahn@kribb.re.krEcogenomics and ecoinformatics of microalgae andcyanobacterial bloomJae Cheol Jeong jcjeong@kribb.re.krTransgenic plants with enhanced tolerance to droughtstress for combating desertificationIl-Gin Mok mokig@kribb.re.krMolecular breeding of sweetpotato and potato plants forsustainable agricultureRESEARCH AREASPlant antioxidation researchDeveloping industrial transgenic plants with enhancedtolerance to multiple environmental stresses forsustainable agriculture on marginal landsMicroalgae researchUsing diverse microalgae in carbon dioxidesequestration and developing environmentally-friendlyproduction technologies for useful materialsMicrobial community researchDeveloping functional microbial communities forbioremediation of contaminated soil and monitoringmicrobial diversity and functionsBiomaterials researchScreening, characterization, and mode-of-action studiesof bioactive substances from microorganisms, plants,and other environmental sourcesDirector Hee-Mock OhT + 82-42-860-4321F + 82-42-879-8103E heemock@kribb.re.krACHIEVEMENTSDevelopment of industrial crops with enhanced tolerance to multipleenvironmental stressesVarious transgenic crops (e.g. sweetpotatoes, potatoes, poplars) through the genemanipulation of their antioxidative mechanisms were developed for sustainableagriculture on marginal lands. Root-specific promoters are under study for the productionof bioenergy and functional feed materials in transgenic sweetpotato plants.Greenhouse gas reduction and beneficial material productionOutdoor mass bioreactors and microalgae harvesting apparatus were developed whichexhibited improved efficiency in carbon dioxide fixation. The resultant microalgae biomasscan be used in biofuels, health foods, cosmetics, and medicines.Development of functional microbial communities for bioremediationDozens of functional microbial communities (FMCs), capable of degrading petroleum oil,were obtained from oil-contaminated soil. We developed an effective technologies for FMCpreservation and field applications.New bioactive metabolites from microorganismsMicroorganisms, including microalgae and cyanobacteria, are rich in secondarymetabolites with diverse chemical structures and various biological functions. Newinhibitors of bacterial fatty acid synthase (FabI, K, G) and peptide deformylase were foundin microorganisms for the first time in this study and have potential for newantibacterials.Environmentally safe natural bioactive substances from plantsBioactive lipid compounds that enhance multi-resistances in plants to abiotic and bioticstresses were found and are being applied to agricultural fields.SELECTED PUBLICATIONSHee-Mock Oh (Corresponding) Bioresour Technol. 101(Suppl 1):S71-4.Selection of microalgae for lipid production under high levels carbon dioxideHee-Sik Kim (Corresponding) Biotechnol Lett. 32(12):1829-35.Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbialenrichment culture developed from sedimentSang-Soo Kwak (Corresponding) Chemosphere. 81(1):79-85.Differential responses of sweetpotato peroxidases to heavy metalsWon-Gon Kim (Corresponding) Food Chem. 123(2):501-6.Isolation and identification of pentagalloylglucose with the broad-spectrum of antibacterialactivity from Rhus trichocarpa MiquelHaeng-Soon Lee (Corresponding) Physiol Plant. 140(2):153-62.Enhanced tolerance to methyl viologen-induced oxidative stress and high temperature intransgenic potato plants overexpressing the CuZnSOD, APX and NDPK2 genesWe aim to develop industrial platform technologies using high-tech ecogenomics and biological resources in response to theUnited Nations Framework Convention on Climate Change. We also investigate water pollution and soil pollution forsustainable development. To achieve these goals, we focus on the development of integrated fusion technologies combinedwith plant science, microbial science (including microalgae), and environmentally-friendly materials science.44 KRIBB Annual Report 2010KRIBB Annual Report 2010 45