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Prefaceixcolleagues demonstrate how to use the deletion of genes in the chicken B cellline DT40 to determine gene function. In chapter 12, Zhang and colleaguesdescribe a retroviral-based short hairpin RNA delivery system for knockingdown genes in mammalian systems. In chapter 13, Cheng and Chang describe aDNA vector-based short hairpin RNA system for inhibiting gene activities in aninheritable or inducible manner. In chapter 14, Hust and colleagues discussmethods to select antibodies for specific proteins, permitting users to determinewhere and when proteins are present and active in their systems. In chapters 15and 16, Tikhmyanova, Serebriiski, and colleagues present two modifications ofyeast two-hybrid protein interaction traps that utilize a linked yeast-bacterialapproach for refining identification of protein interaction partners. In chapter17, Thibodeau-Beganny and Joung describe how to use bacterial two-hybridtechnology to select Cys2His2 zinc finger domains with specific properties.Michael F. Ochs
Page 2: Gene Function Analysis
Page 6: METHODS IN MOLECULAR BIOLOGYGene Fu
Page 12: PrefaceThis volume of Methods in Mo
Page 18: ContentsPreface ...................
Page 22: ContributorsPETER D. ADAMS • Fox
Page 32: Gene Function Inference in Deletion
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Page 64: 2Association Analysis for Large-Sca
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Table 1Tools That Can Perform Gene
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Association Analysis for Large-Scal
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36 Wang and Ochsfunction (9). Herei
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38 Wang and Ochs1. Download the LS-
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40 Wang and OchsFig. 1. The PattRun
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42 Wang and OchsFig. 3. The PattRun
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44 Wang and OchsFig. 4. The gene ta
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46 Wang and Ochsresults posttreatme
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4From Promoter Analysis to Transcri
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Prediction Using PAINT 51even in si
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Prediction Using PAINT 53Fig. 1. A
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Prediction Using PAINT 55first exon
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58 Gonye et al.Fig. 3. A network vi
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60 Gonye et al.exGeneList.txt) is a
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62 Gonye et al.(http://www.tm4.org)
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64 Gonye et al.does not span the en
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66 Gonye et al.4.7. Interpreting th
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68 Gonye et al.18. Dozmorov, M. G.,
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70 Uversky et al.in protein functio
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72 Uversky et al.sequence space and
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Table 1 (Continued)Server name URL
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76 Uversky et al.1.5. When to Use t
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78 Uversky et al.elucidating compos
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Table 2Averaged Frequencies of Diff
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82 Uversky et al.the box Raw Output
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84 Uversky et al.peculiarities of t
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86 Uversky et al.Fig. 4. Prediction
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88 Uversky et al.13. Uversky, V. N.
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90 Uversky et al.50. Vucetic, S., O
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92 Uversky et al.84. Ritter, L. M.,
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6Sybil: Methods and Software for Mu
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Sybil: Multiple Genome Comparison a
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7Estimating Protein Function Using
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Estimating Protein Function Using P
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130 Davuluriinteracting proteins an
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Table 1Web URLs of Promoter, TF Dat
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134 DavuluriPWM-based models do not
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136 DavuluriTF-map alignments of or
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138 Davuluridiscussed which program
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140 DavuluriTable 2ER-a-Responsive
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Table 3Sample Data Matrix Represent
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Table 3 (Continued)Class MYCMAX MYC
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146 DavuluriFig. 3. (A) CART Tree:
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148 Davuluri11. Vlieghe, D., Sandel
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150 Davuluri44. Berezikov, E., Gury
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9Mining Biomedical Data Using MetaM
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Mining Biomedical Data Using MMTx a
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172 Ho et al.Fig. 1. Artificial exa
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174 Ho et al.allowing for cases whe
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176 Ho et al.A different measure is
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178 Ho et al.3.1.3. LA and Generali
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180 Ho et al.The ECF-statistic can
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182 Ho et al.In the special case of
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184 Ho et al.Fig. 5. An illustratio
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186 Ho et al.Fig. 7. The power curv
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188 Ho et al.this section were not
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190 Ho et al.References1. Schena, M
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IIIEXPERIMENTAL METHODS
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194 Caldwell et al.for sequences th
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196 Caldwell et al.query because it
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198 Caldwell et al.Fig. 1. (A) Prot
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200 Caldwell et al.outside primer o
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202 Caldwell et al.5. Targeting scr
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204 Caldwell et al.will allow the s
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206 Caldwell et al.3.1.6. Plasmid P
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208 Caldwell et al.PCR amplify the
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210 Caldwell et al.8. Thawing cells
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212 Zhang et al.Going one step beyo
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214 Zhang et al.Fig. 2. Generation
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216 Zhang et al.Perform PCR cycles,
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218 Zhang et al.Fig. 4. Schematic m
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220 Zhang et al.Fig. 5. Replacement
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13Construction of Simple and Effici
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DNA Vector-Based shRNA-Expression S
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244 Hust et al.overcome by two appr
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246 Hust et al.Fig. 1. Schematic de
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248 Hust et al.interaction during p
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250 Hust et al.3.4. Titering1. Inoc
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252 Hust et al.10. Shortly before u
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254 Hust et al.activity by preservi
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15A Bacterial/Yeast Merged Two-Hybr
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Screening in Yeast With a Bacterial
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16A Bacterial/Yeast Merged Two-Hybr
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Dual Bait-Compatible Bacterial Two-
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318 Thibodeau-Beganny and Joungbeen
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320 Thibodeau-Beganny and JoungFig.
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324 Thibodeau-Beganny and JoungTypi
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328 Thibodeau-Beganny and JoungPCR
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330 Thibodeau-Beganny and Joung16-1
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332 Thibodeau-Beganny and Joung2. P
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334 Thibodeau-Beganny and Joung11.
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336 IndexKknockin (gene knockin) 19
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