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Dual Bait-Compatible Bacterial Two-Hybrid System 31511. Struhl, K., Cameron, J. R., and Davis, R. W. (1976) Functional genetic expressionof eukaryotic DNA in Escherichia coli. Proc. Natl. Acad. Sci. USA 73, 1471–1475.12. Struhl, K. and Davis, R. W. (1977) Production of a functional eukaryotic enzymein Escherichia coli: cloning and expression of the yeast structural gene forimidazole-glycerolphosphate dehydratase (his3). Proc. Natl. Acad. Sci. USA 74,5255–5259.13. Brennan, M. B. and Struhl, K. (1980) Mechanisms of increasing expression of ayeast gene in Escherichia coli. J. Mol. Biol. 136, 333–338.14. Hollingshead, S. and Vapnek, D. (1985) Nucleotide sequence analysis of a geneencoding a streptomycin/spectinomycin adenylyltransferase. Plasmid 13, 17–30.15. Giesecke, A. V. and Joung, J. K. (2005) Bacterial Two-hybrid System forStudying Protein-Protein Interactions, in Protein-Protein Interactions: A MolecularCloning Manual, (Golemis, E. A., ed.), Cold Spring Harbor Laboratory Press,Cold Spring Harbor, NY, pp. 195–216.16. Miller, J. H. (1992) A short course in bacterial genetics, 1st ed., Cold SpringHarbor Laboratory Press, Cold Spring Harbor, NY.17. Sambrook, J. and Russell, D. (eds.), (2001) Molecular cloning: a laboratorymanual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.18. Thibodeau, S. A., Fang, R., and Joung, J. K. (2004) High-throughput beta-galactosidaseassay for bacterial cell-based reporter systems. Biotechniques 36,410–415.19. Serebriiskii, I. G. and Golemis, E. A. (2000) Uses of lacZ to study gene function:evaluation of beta-galactosidase assays used in the yeast two-hybrid system.Anal. Biochem. 285, 1–15.20. Hartley, J. L., Temple, G. F., and Brasch, M. A. (2000) DNA cloning using invitro site-specific recombination. Genome Res. 10, 1788–1795.21. Walhout, A. J., Temple, G. F., Brasch, M. A., et al. (2000) GATEWAY recombinationalcloning: application to the cloning of large numbers of open readingframes or ORFeomes. Methods Enzymol. 328, 575–592.22. Meng, X., Smith, R. M., Giesecke, A. V., Joung, J. K., and Wolfe, S. A. (2006)Counter-selectable marker for bacterial-based interaction trap systems.Biotechniques 40, 179–184.23. Stanyon, C. A., Limjindaporn, T., and Finley, R. L., Jr. (2003) Simultaneous cloningof open reading frames into several different expression vectors. Biotechniques 35,520–522, 524–526.

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