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Estimating Protein Function Using Protein–Protein Relationships 113MSLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEICDAE>hsapiens|gi|20093442MGVIEDMMKVGMRSAKAGLEATEELIKLFREDGRLVGSILKEMEPEEITELLEGASSQLIRMIR>hsapiens|gi|20093443MSGNFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTACQLVQ>>celegans|gi|1453778MEYIYAALLLHAAGQEINEDNLRKVLEAAGVDVDDARLKATVAALEEVDIDEAIEEAAVPAAAP>celegans|gi|1453779MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYALAHD>scerevisiae|gi|6799765MAEHELRVLEIPWVEKYRPKRLDDIVDQEHVVERLKAYVNRGDMPNLLFAGPPGTGKTTAALCLThe contents of this file are in the “FASTA” format, wherein lines startingwith the “>” sign are treated as comments (for more details, seehttp://www.ebi.ac.uk/ help/formats_frame.html) and lines that do not start withthe “>” sign are treated as sequence. For the proteins described in the mockdatabase above, the comment line contains an abbreviation of the organismname (the first letter of the genus name concatenated with the entire speciesname), followed by the words “gi,” which alert the user to the fact that the followingidentifier is a Genbank identifier. The identifier associated with eachsequence is unique, and can be used to retrieve records from the NCBI website.This comment structure is used here just for illustration purposes, andother more suitable formats can be envisioned depending on need.Advanced users familiar with this step and the subsequent database formattingstep for BLAST can choose to construct more advanced databases, such asthose with indices. If an advanced database is to be created, users are advisedto carefully follow instructions with respect to identifiers associated with theindividual sequences.3.1.2. Formatting the Database for Use by BLASTThe database of reference genomes requires formatting before it can be usedfor sequence comparison by BLAST. A special program called “formatdb,”included with the BLAST tools package, is needed for this task. A number ofoptions can be set for formatdb, depending on the type of input and outputdesired. However, for this protocol, as the input is a file containing amino acidsequences and no additional information is to be generated, no options need tobe specified for formatdb (i.e., formatdb is run with default options):% /path/to/blast/package/formatdb –imyDatabaseFile
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Gene Function Analysis
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METHODS IN MOLECULAR BIOLOGYGene Fu
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PrefaceThis volume of Methods in Mo
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Prefaceixcolleagues demonstrate how
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xiiContentsPART III EXPERIMENTAL ME
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ICOMPUTATIONAL METHODS I
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4 BidautTable 1Input File Format Us
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6 BidautTable 2Folder Layout to Use
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8 Bidaut• alphaA: this is the num
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10 Bidautcomputing the maximum corr
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12 BidautFig. 3. The complete Clutr
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Table 3Some Identified Patterns (5,
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16 BidautFig. 4. This is a comparis
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18 BidautReferences1. Hughes, T. R.
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20 Kirov et al.way to associate gen
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22 Kirov et al.based on a study ass
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24 Kirov et al.1. Retrieve the gene
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26Fig. 1. Functional associations f
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28 Kirov et al.Fig. 2. Pathway anal
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30 Kirov et al.3. Gene symbols usag
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32 Kirov et al.9. OBO_Team, Open Bi
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3Estimating Gene Function With Leas
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Estimating Gene Function With LS-NM
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50 Gonye et al.activity and problem
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52 Gonye et al.Currently, PAINT can
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54 Gonye et al.dynamic nature of th
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Prediction Using PAINT 57represente
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Prediction Using PAINT 59In PAINT,
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Prediction Using PAINT 6114. On the
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Prediction Using PAINT 634.2. Size
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65Fig. 4. Localization of enrichmen
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Prediction Using PAINT 673. Okubo,
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5Prediction of Intrinsic Disorder a
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Prediction of ID and Its Use in Fun
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Table 1Summary of the Web Servers O
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Page 208: IICOMPUTATIONAL METHODS II
Page 212: 94 Crabtree et al.genomes, which is
Page 216: 96 Crabtree et al.Fig. 2. Sybil pro
Page 220: 98 Crabtree et al.Fig. 3. Computing
Page 224: 100 Crabtree et al.3.1.5.1. FILTER
Page 228: 102 Crabtree et al.3. For the sake
Page 232: 104 Crabtree et al.Fig. 5. Best bid
Page 236: 106 Crabtree et al.17. Some cluster
Page 240: 108 Crabtree et al.19. Chado—The
Page 244: 110 Dateproducts prevents the under
Page 248: 112 DateDetails of these tasks are
Page 254: Estimating Protein Function Using P
Page 282: 130 Davuluriinteracting proteins an
Page 286: Table 1Web URLs of Promoter, TF Dat
Page 290: 134 DavuluriPWM-based models do not
Page 294: 136 DavuluriTF-map alignments of or
Page 298: 138 Davuluridiscussed which program
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140 DavuluriTable 2ER-a-Responsive
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Table 3Sample Data Matrix Represent
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Table 3 (Continued)Class MYCMAX MYC
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146 DavuluriFig. 3. (A) CART Tree:
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148 Davuluri11. Vlieghe, D., Sandel
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150 Davuluri44. Berezikov, E., Gury
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9Mining Biomedical Data Using MetaM
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Mining Biomedical Data Using MMTx a
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172 Ho et al.Fig. 1. Artificial exa
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174 Ho et al.allowing for cases whe
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176 Ho et al.A different measure is
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178 Ho et al.3.1.3. LA and Generali
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180 Ho et al.The ECF-statistic can
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182 Ho et al.In the special case of
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184 Ho et al.Fig. 5. An illustratio
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186 Ho et al.Fig. 7. The power curv
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188 Ho et al.this section were not
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190 Ho et al.References1. Schena, M
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IIIEXPERIMENTAL METHODS
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194 Caldwell et al.for sequences th
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196 Caldwell et al.query because it
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198 Caldwell et al.Fig. 1. (A) Prot
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200 Caldwell et al.outside primer o
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202 Caldwell et al.5. Targeting scr
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204 Caldwell et al.will allow the s
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206 Caldwell et al.3.1.6. Plasmid P
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208 Caldwell et al.PCR amplify the
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210 Caldwell et al.8. Thawing cells
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212 Zhang et al.Going one step beyo
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214 Zhang et al.Fig. 2. Generation
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216 Zhang et al.Perform PCR cycles,
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218 Zhang et al.Fig. 4. Schematic m
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220 Zhang et al.Fig. 5. Replacement
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13Construction of Simple and Effici
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DNA Vector-Based shRNA-Expression S
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244 Hust et al.overcome by two appr
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246 Hust et al.Fig. 1. Schematic de
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248 Hust et al.interaction during p
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250 Hust et al.3.4. Titering1. Inoc
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252 Hust et al.10. Shortly before u
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254 Hust et al.activity by preservi
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15A Bacterial/Yeast Merged Two-Hybr
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Screening in Yeast With a Bacterial
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16A Bacterial/Yeast Merged Two-Hybr
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Dual Bait-Compatible Bacterial Two-
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318 Thibodeau-Beganny and Joungbeen
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320 Thibodeau-Beganny and JoungFig.
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324 Thibodeau-Beganny and JoungTypi
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328 Thibodeau-Beganny and JoungPCR
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330 Thibodeau-Beganny and Joung16-1
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332 Thibodeau-Beganny and Joung2. P
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334 Thibodeau-Beganny and Joung11.
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336 IndexKknockin (gene knockin) 19
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