SUNDAY, APRIL 22 Across Societies – Experimental Biology

BIOCHEMISTRYA236 581.5 Evidence for the presence of phosphate groupson the two major proteins of the hamster sperm acrosomalmatrix and their potential role in acrosomal hydrolase bindingactivities. G. Alvarez, T.L. Buchanan, S. Raychoudhury andS.K. Nagdas. Fayetteville State Univ., NC and Benedict Col.,SC.A237 581.6 Glycoprotein changes in the bovine spermplasma membrane during maturation in the epididymis. T.L.Buchanan, E. McLean, S. Raychoudhury and S.K. Nagdas.Fayetteville State Univ., NC and Benedict Col., SC.A238 581.7 Development of analytical methods forcharacterization of Fab fragments. L.C. Harwick, J.R.Fishpaugh and K.R. Rupprecht. Abbott Labs.A239 581.8 Creation of a new protein by substituting allarginine residues by its toxic analogue, canavanine. Y. Ishida,J-h. Park and M. Inouye. Robert Wood Johnson Med. Sch.A240 581.9 The roles of conserved cysteine residues in thestructure and function of glyoxysomal malate dehydrogenase.R. Jones and E. Bell. Univ. of Richmond and Math. & Sci. HighSch. at Clover Hill, VA.A241 581.10 Adhesion of albumin to FDA type II soft contactlenses. Y. Harkas, M. Harkas, J. Nichols, D. Cooper, A. Janoffand E.O. Keith. Nova Southeastern Univ. Col. of Arts and Sci.and Col. of Optom.A242 581.11 High selectivity purifi cation screening ofhistidine-tagged proteins using small sample preparationformats containing cobalt IMAC media. M. Björner, J.Lundqvist and H. Hedlund. GE Healthcare, Uppsal, Sweden.A243 581.12 Stability and functionality of HL heteropolymerferritins responsible for a hereditary ferritinopathy disorder. J.McNally, P. Arosio and F. Bou-Abdallah. SUNY Potsdam andUniv. of Brescia, Italy.A244 581.13 The linkage between citrate, pH regulation andprotein folding in glyoxysomal malate dehydrogenase. H. Yantaand E. Bell. Univ. of Richmond.582. ELECTROPHORESISPosterSUN. 7:30 AM—SAN DIEGO CONVENTION CENTER, EXHIBITHALLS A-DPresentation time: 1:05 PM-2:35 PMA245 582.1 Operation invisible dead: a blind study tocompare molecular fi ngerprints of soil samples between crimescene and criminal’s shoe. N. Patel, S. Larson, V. Freemanand C. Bailey. Univ. of Nebraska-Lincoln.583. NEW MOLECULAR AND CELLULAR IMAGINGAGENTSPosterSUN. 7:30 AM—SAN DIEGO CONVENTION CENTER, EXHIBITHALLS A-DPresentation time: 1:05 PM-2:35 PMA246 583.1 Ultrasensitive, background-free single copyDNA detection: benchmarking performance using HPV infectedcell lines and tissue biopsies. J. Coleman, I. Lebedeva, M.Szczepanik, P. Pande, F. Liang and W. Patton. ENZO LifeSci., Farmingdale, NY.A247 583.2 New cell biology assays using a stabilizedluciferase exhibiting red luminescence. Y. Jiang and J.J.Naleway. Marker Gene Technol. Inc., Eugene, OR.584. RECOMBINANT DNA TECHNOLOGYPosterSUN. 7:30 AM—SAN DIEGO CONVENTION CENTER, EXHIBITHALLS A-DPresentation time: 1:05 PM-2:35 PMA248 584.1 Expresso ® cloning and expression systemsstreamline recombinant protein expression. S. Sen, H.Sternard, L. Franz, C. Drinkwater, L. Sheets, C. Niebauer,D. Mead, R. Godiska and E.J. Steinmetz. Lucigen Corp.,Middleton, WI.A249 584.2 Molecular cloning of a dithiol glutaredoxincDNA from sweet potato. L. Wen, X-W. Chi, C-T. Lin, Y-C.Jiang and C-T. Lin. Western Illinois Univ., Natl. Taiwan OceanUniv. and Natl. Changhua Univ. of Educ., Taiwan.A250 584.3 A novel approach to isolate unselectedrecombinants facilitates markerless DNA recombineering. G.Lezin, Y. Kosaka, H.J. Yost, M.R. Kuehn and L. Brunelli. Univ.of Utah and NCI-Frederick.A251 584.4 Differentiating frequency of recombination fromfrequency of recombinants. G. Lezin, Y. Kosaka, H.J. Yost,M.R. Kuehn and L. Brunelli. Univ. of Utah and NCI-Frederick.A252 584.5 Cloning of a putative L-3-hydroxyacyl CoAdehydrogenase from Micrococcus luteus. V. Takkalapalli, J-K.Huang and L. Wen. Western Illinois Univ.585. MITOCHONDRIAL DYNAMICSPosterSUN. 7:30 AM—SAN DIEGO CONVENTION CENTER, EXHIBITHALLS A-DPresentation time: 12:25 PM-1:55 PMSUNDAYA253 585.1 Molecular nanoenvironment of themitochondrial presequence translocase. C.S. Mehnert, M.Gebert, S. Oeljeklaus, N. Pfanner and M. van der Laan. Univ.of Freiburg, Germany.A254 585.2 Lithocholic acid delays yeast aging by alteringmitochondrial dynamics. V. Titorenko, A. Beach and M.Burstein. Concordia Univ., Canada.A255 585.3 Identifi cation and characterization of novelfactors regulating kinesin-1 activation and mitochondriadynamics. K. Cho, H. Patil and P. Ferreira. Duke Univ. Med.Ctr.A256 585.4 Modeling dynamic regulation of mitochondrialfree Ca 2+ : effects of Ca 2+ sequestration and precipitation. R.K.Pradhan, C.A. Blomeyer, J. Bazil, A.K.S. Camara, D.F. Stoweand R.K. Dash. Med. Col. of Wiscosnsin.A257 585.5 Cardiac-specifi c deletion of prohibitin-2 inadult mice destabilizes mitochondrial proteins, nucleoids,and cristae morphology, resulting in lethal cardiomyopathy. T.Gawlowski, J. Yan, C. Merkwirth, W. Dillmann, T. Langer andM. Hoshijima. UCSD and Univ. of Cologne, Germany.A258 585.6 A novel mitochondrial ubiquitin ligase involvedin the regulation of mitochondrial fusion. A-S. Benischke,C. Hemion, M. Neutzner, K. Norris, S. Frank, R. Youle, M.Karbowski and A. Neutzner. Univ. of Basel, NIH and Univ. ofMaryland Biotechnol. Inst.158