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4404 J. Med. Plants Res. A B Figure 1. Callus formation and in vitro shoot regeneration of Tribulus terrestris L., a) direct shoot regeneration for epicotyl on MS media supplemented with 0.1 mg/l NAA + 2 mg/l BAP and b) indirect regeneration of shoots from the hypocotyls on medium with 0.4 mg/l NAA + 2 mg/l BAP. Table 3. Effect of different concentration of NAA and BAP on shoot regeneration from hypocotyl and epicotyl of Tribulus terrestris after 14 to 21 days of culture. Plant regulator concentration Explant producing shoot NAA BAP Epicotyl (%) Hypocotyls (%) Leaf (%) 0.1 0.2 0.4 0.5 0 0 0 1 0 0 0 2 22 b 10 d 0 0.5 0 0 0 1 0 0 0 2 0 0 0 0.5 22 b 0 0 1 0 0 0 2 15.3 c 28.4 a 0 Value within a column followed by different letters are significantly different at the 0.05 probability level, analyzed by Duncan’s multiple range test. Shoots were excised from the explants and subculture on MS medium for the development of plantlets. To promote the development of roots, the plantlets were transferred to MS medium alone or supplemented with 2 mg/l IBA (Figure 3a). The rooting of regenerated and elongated shoots was observed on MS with IBA and without IBA after 14 to 30 days of culture and the percentage of rooted shoots fluctuated between 70 and 100%. Rooted plantlets were then transferred to a bed of sterile, moist sand and perlite (Figure 3b). DISCUSSION Due to its high medicinal value and increasing demands, in vitro studies have great importance in the propagation and genetic improvement programmer in this species. In nature, there are many mechanisms producing the crack of the tegumentary barrier in legumes, as temperature oscillation and the alternance of dry and wet periods (Quinlivan, 1971; Roslton, 1978), bacteria and other soil microrganism action, and the chemical
A Figure 2. Shoot multiplication from node explants on MS medium with 3 mg/l BA after 4 weeks. Table 4. Effect of BA (cytokinin) on shoot induction from nodal explants of Tribulus terrestris. BA (mg/l) Explant initiating shoots (%) Number of shoots/explant 3.0 54 a 2.53 a 4.0 46 ab 2.06 a 5.0 30 b 2.53 a Value within a column followed by different letters are significantly different at the 0.05 probability level, analyzed by Duncan’s multiple range test. B Sharifi et al. 4405 Figure 3. a) Transferring of regenerated shoots to hormone-free MS medium for developing and rooting after 4 weeks and b) transferring rooted plantlets to soil. scarification suffered through the herbivore digestive system (Pereiras et al., 1985). In the current study, it seems that soil bacteria and other microrganisms stimulate germination of T. terrestris but physical scarification of seed coat and chemical treatment (with H2SO4) did not have any effect on germination. Petkov (2010) reported that cyanobacteria and microalgae, being photosynthesizing organisms, enrich the soil with oxygen, thus increased seed germination of T. terrestris.
Page 1 and 2: B Journal of Medicinal Plants Resea
Page 3 and 4: Editors Prof. Akah Peter Achunike E
Page 5 and 6: Electronic submission of manuscript
Page 7 and 8: Fees and Charges: Authors are requi
Page 9 and 10: ences Table of Contents: Volume 6 N
Page 11 and 12: supplement of dietary minerals. The
Page 13 and 14: several studies. MO leaves ethanoli
Page 15 and 16: (Nandave et al., 2009). In ocular d
Page 17 and 18: Journal of Medicinal Plants Researc
Page 19 and 20: Table 1. Pa and Pi of PTK inhibitor
Page 23 and 24: Table 1. Plant collection and stora
Page 25 and 26: Table 2. Count’d. Psydrax livida
Page 27 and 28: Mukandiwa et al. 4385 Figure 3. Chr
Page 29 and 30: Euphorbiaceae family to which S. af
Page 33 and 34: Table 1. Number of axillary shoots
Page 35 and 36: REFERENCES Hui et al. 4393 Figure 1
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Page 39 and 40: Table 2. Analysis of variance of yi
Page 41 and 42: Pirzad 4399 Table 3. Correlation co
Page 45: Table 1. Analysis of variance (ANOV
Page 49 and 50: capacity could be a model for micro
Page 53 and 54: Table 1. 13 C-NMR data for compound
Page 55 and 56: Figure 1. Chemical structures of co
Page 57 and 58: potent activity against the HepG2 c
Page 59 and 60: Lippia spp. using gas chromatograph
Page 61 and 62: Table 1. Contd. Singulani et al. 44
Page 63 and 64: Previously, β-caryophyllene was id
Page 67 and 68: consumers' individual characteristi
Page 69 and 70: Table 3. Contd. Hong et al. 4427 C2
Page 73 and 74: RESULTS Buncharoen et al. 4431 Tabl
Page 75 and 76: Buncharoen et al. 4433 Figure 2. Ph
Page 77 and 78: Stemona curtisii Hkf. Proceeding of
Page 79 and 80: Table 1. Herbal tea samples. Aboule
Page 81 and 82: Figure 2. Chloride levels in herbal
Page 83 and 84: Table 3. Herbal tea ingredients and
Page 87 and 88: Mean feed intake (g) 6 5 4 3 2 1 0
Page 89 and 90: Percentage mortality Percentage mor
Page 91 and 92: involvement according to Okoye et a
Page 93 and 94: properties of green leafy vegetable
Page 95 and 96: Table 1. Percentage yield extracts
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