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Value added fish by-products - Nordic Innovation

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Figure 3.5. Sensorial attributes of <strong>fish</strong> protein isolates made from Arctic charr, saithe and cod, respectively.<br />

Evaluation of the effects of salt concentration, cryoprotectants and chilled and frozen<br />

storage on physical properties of cod protein solutions and haddock protein isolate.<br />

In the second test influence of variation in salt concentration, cryoprotectants and chilled and<br />

frozen storage of cod protein solutions (CPS) and haddock protein isolate (HPI) was measured<br />

with regard to viscosity, colour, water holding capacity and stability (microbial count, TVB-<br />

N). The <strong>fish</strong> protein solutions and <strong>fish</strong> protein isolate were extracted from cut-offs of cod<br />

(Gadus morhua) and haddock (Melanogrammus aeglefinus), respectively, using the pH-shift<br />

process.<br />

The results indicate that <strong>fish</strong> protein solutions and <strong>fish</strong> protein isolates are affected <strong>by</strong><br />

different amounts of additives (salt and cryoprotectants). The physicochemical and<br />

rheological properties of these <strong>products</strong> depend on the additives and time and temperature of<br />

storage. Using cryoprotectants for CPS and a mixture of salt and sucrose for HPI were<br />

recommended to stabilise these <strong>products</strong>. The most stable frozen cod protein solution was<br />

with 5% salt and cryoprotectants followed <strong>by</strong> the solution with 3% salt and cryoprotectants.<br />

To make <strong>fish</strong> protein solutions that would be stable during frozen storage it is recommended<br />

to add cryoprotectants to the <strong>products</strong> (preferably containing 3-5% salt) at the end of the pH-<br />

shift process. The cryoprotectants increased the water holding capacity and viscosity<br />

(Brabender and Pascal), decreased the weight loss and improved whiteness in CPS containing<br />

1.2, 3, 5 and 15% salt. Shelf life of <strong>fish</strong> protein solution containing 3-5% protein can be<br />

extended <strong>by</strong> thermal processing (pasteurization) but risking the loss of desirable functional<br />

properties of the protein solution.<br />

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