IARA Vol. XXVI No. 1 Year. 2009 (pdf, 1420 kb) - Pustaka Deptan

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Indonesian Agricultural Research Abstracts Vol. 26, No. 1, 2009COWS; BREEDS (ANIMALS); SUPPLEMENTS; PROGESTERONE; PROLACTIN; NUTRIENTS.The influence of multinutrient blocks during pre and postcalving on progesterone and prolactin profiles ofbali cows were evaluated. Seventeen multiparous pregnant cows with BCS 1 to 2, approximately 90 daybefore the expected date of calving, were divided randomly into groups A (n=9) and B (n=8), and weregrazed on the native pasture as a basal diet, while those of group B received 1.25 kg multinutrient blocks,whose constitute was as follows (%): molasses (28), urea (5), coconut cake (15), fishmeal (5), rice bran(25), lime (8.5), salt (7.5), grit (5) and ultramineral (1). Cows were weighed and assessed for BCS (on afive-point scale) every two weeks, commencing at 12 weeks prior to calving, within 24 day after calvingup to 16 weeks after calving. Plasma progesterone was measured twice weekly using RIA procedure whileserum prolactin concentrations was measured using an ELISA at weekly interval. Bali cow grazing onnatural pasture had a similar pattern of prepartum progesterone profile to bali cows supplemented withmultinutrient blocks. Plasma progesterone of both groups was low after parturition, but 4 cows from nonsupplementedand 5 cows from supplemented group had a transient rise of progesterone prior to the firstestrus. Supplementation with multinutrient blocks to pre and postcalving bali cows grazing on naturalpasture did not affect serum prolactin concentration. The fluctuation of serum prolactin levels were stillsubstantially lower than that occurred at calving. The magnitude of prolactin concentrations in bali cows ismuch lower than that in other breeds. Plasma progesterone concentrations between the two groups aroundprecalving period were not significantly affected by the multinutrient block.116 SAID, S.Live capacity of fresh spermatozoa of simmental cattle which is kept in straw at temperature 5°C.Daya tahan hidup sperma cair sapi Simmental yang disimpan dalam straw pada temperatur 5°C/Said,S.; Gunawan, M.; Kaiin, E.M.; Tappa, B. (Pusat Penelitian Bioteknologi-LIPI, Bogor (Indonesia)).[Proceedings of the national seminar on animal husbandry and veterinary technology. Book 1], Bogor(Indonesia) 12-13 Sep 2005/Mathius, I W.; Bahri, S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.;Tiesnamurti, B.; Sendow, I.; Suhardono (eds.) Pusat Penelitian dan Pengembangan Peternakan, Bogor(Indonesia). Bogor: Puslitbangnak, 2005: p. 87-90, 5 tables; 11 ref. 636:338.439/SEM/pCATTLE; SPERMATOZOA; STORAGE; STRAW; TEMPERATURE.This research was conducted to know live capacity of fresh spermatozoa of simmental cattle in tris-eggyolk dilution (TKT, 20% v/v) which is kept in straw at 5°C (K 1). Examination of live capacity wasconducted every day until fourth day, including: percentage of motility, live cell, abnormality and plasmamembrane integrity. Fresh semen placed in glass bottle as control (K0). Results of this research indicatedthat motility and live cell of fresh spermatozoa after fourth days Kl (20.63% and 56.98%, respectively)were higher compared to control K0 (15.63% and 54.89%, respectively). Furthermore, percentage ofabnormality and plasma membrane integrity after fourth days K0 (9.95% and 65.03%, respectively) washigher compared to Kl (9.18% and 61.49%, respectively). These result indicated that live capacity freshspermatozoa of simmental cattle kept in straw at 5°C was better than that in glass bottle and can be usedfor insemination until on third day.L70 VETERINARY SCIENCE AND HYGIENE – GENERAL ASPECTS117 ARIYANTI, T.Development enzyme linked immunosorbent assay for evaluating antibody response of egg yolkfrom chicken immunized with killed whole cell antigen of Salmonella enteritidis phage type 4.Pengembangan enzyme linked immunosorbent assay untuk evaluasi respon antibodi pada egg yolk dariayam yang diimunisasi antigen sel utuh inaktif S. enteritidis phage type 4/Ariyanti, T.; Supar; Djaenuri;Iskandar (Balai Penelitian Veteriner, Bogor (Indonesia)). [Proceedings of the national seminar on animalhusbandry and veterinary technology], Bogor (Indonesia) 12-13 Sep 2005/Mathius, I W.; Bahri, S.;Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.; Tiesnamurti, B.; Sendow, I.; Suhardono (eds.) Pusat57
Vol. 26, No. 1, 2009Indonesian Agricultural Research AbstractsPenelitian dan Pengembangan Peternakan, Bogor (Indonesia). Bogor: Puslitbangnak, 2005: p. 1056-1069,6 ill., 2 tables; 20 ref. 636:338.439/SEM/pCHICKENS; EGG YOLK; ELISA; ANTIBODIES; MASTIGOPHORA; SALMONELLAENTERITIDIS; IMMUNOLOGICAL TECHNIQUES.Salmonellosis is an important disease in poultry industries that may occur in the animal level at farms aswell as in the laying eggs or egg product. S. enteritidis phage type 4 is one of the most important serotypecausing disease in chicken which could spread vertically through the eggs as well as horizontally by directcontact. Detection of S. enteritidis phage type 4 infections or its antibodies in eggs are important andappropriate for reducing egg-borne disease transmission. At present, enzyme linked immunosorbent assaywas developed to detect the presence of S. enteritidis phage type 4 antibody responses in egg derived fromexperimental chicken against somatic antigen (0), extracellular toxin and H:g,m flagella antigen. A groupof 14 weeks old of layer chicken (group I) were immunized with killed whole cell antigen of S. enteritidisphage type 4 isolated from Sukabumi. A group of the same age of other chickens (group II) was leftunimmunized used as control. Each of that group were divided into 3 subgroups designated as IA, IB, ICand IIA, IIB, IIC, respectively. Two weeks postboostered, subgroup IB and IIB were challenged with lifehomologous of S. enteritidis phage type 4 whereas subgroup IC and IIC were challenged 12 weekspostboostered, subgroup IA, IIA were left unchallenged. ELISA antigen of whole sonicated extract, heatedwhole sonicated extract, extracellular toxins and H:g,m flagella antigen were prepared from homologousS. enteritidis phage type 4 serotype. The enzyme substract reaction of the ELISA were determined byoptical density reading (OD), then converted to ELISA unit based on the positive control. The somatic (0)antibody responses and antitoxin antibody responses were detected from egg yolk of chicken immunizedwith killed whole cell antigen. Anti H:g,m flagella antibody responses could be detected earlier at 14 daysafter immunization. From four types of the ELISAs developed, the H:g,m flagella antigen coating ELISAmicroplates demonstrated specific for detecting anti H:g,m flagella antibody responses from eggs yolk,whereas somatic (O) antigen coating ELISA microplates detected anti-somatic (O) antibody from eggs,produced from chicken injected with other Salmonella serotype of group D. From this study conduted, theuse of H:g,m flagella for coating ELISA microplate could be used for differentiation Salmonellosisinfection status due to the S. enteritidis or other group of D.118 KAIIN, E.M.Quality of sperm after sexing frozen in dynamic and static racks. Kualitas sperma hasil pemisahanyang dibekukan menggunakan rak dinamis dan statis/Kaiin, E.M.; Said, S.; Tappa, B. (Pusat PenelitianBioteknologi LIPI, Bogor (Indonesia)); Ginting, S.S.; Djuarsawidjaja, M. [Proceedings of the nationalseminar on animal husbandry and veterinary technology. Book 1], Bogor (Indonesia) 12-13 Sep2005/Mathius, I W.; Bahri, S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.; Tiesnamurti, B.; Sendow,I.; Suhardono (eds.) Pusat Penelitian dan Pengembangan Peternakan, Bogor (Indonesia). Bogor:Puslitbangnak, 2005: p. 105-111, 5 tables; 21 ref. 636:338.439/SEM/pBULLS; SEMEN; FREEZING; QUALITY.The aim of this study is to evaluate the quality of sperm after sexing and frozen in two different kinds ofracks. Factorial randomized design (2x3) with five replications was used to evaluate the result. A malehungarian bull was used as sperm donor. Semen of the bull was collected using an artificial vagina (AV)and was evaluated macroscopically and microscopically. Separation of sperm was done using a columnalbumin methods, with 5% BSA for the upper fraction and 10% BSA for the lower fraction. Sperm aftersexing was diluted using Tris buffer solution containing 20% egg yolk and 8% glycerol (v/v). Sperm aftersexing in the 0.25 ml straw were frozen in 10 cm above liquid nitrogen using dynamic and static racks for10 minutes. Thawing of straw was done in warm water (37°C) for 30 seconds. Higher percentage of spermmotility was seen at sperm frozen with dynamic racks, for control group (47 %) and separatedspermatozoa (X=44.1% and Y=43.7%) than static racks (control = 39.9%; X=35.4% and Y=36.8%). Thesame result was seen also in percentage of live sperm (control = 51.2%; X = 40.9% and Y = 47.3%) for58
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IARA Vol. XXVI No. 1 Year. 2009 (pdf, 1420 kb) - Pustaka Deptan

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