Indonesian Agricultural Research Abstracts <strong>Vol</strong>. 26, <strong>No</strong>. 1, <strong>2009</strong>139 HARSOJO.Decontamination of some phatogenic bacterials on goat meat and bowel by gamma irradiation.Dekontaminasi bakteri patogen pada daging dan jeroan kambing dengan iradiasi gamma/Harsojo;Andini, L. (Pusat Penelitian dan Pengembangan Teknologi Isotop dan Radiasi, Jakarta (Indonesia));Trimey T., N.R. [Proceedings of the national seminar on animal husbandry and veterinary technology],Bogor (Indonesia) 12-13 Sep 2005/Mathius, I W.; Bahri, S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih,E.; Tiesnamurti, B.; Sendow, I.; Suhardono (eds.) Pusat Penelitian dan Pengembangan Peternakan, Bogor(Indonesia). Bogor: Puslitbangnak, 2005: p. 1027-1031, 4 tables; 17 ref. 636:338.439/SEM/pGOATS; MEAT; BACTERIA; PATHOGENS; SALMONELLA; IRRADIATION; RADIOACTIVEDECONTAMINATION.Goat meat and bowel are consumed a lot by Indonesians to make roast goat meat or curry soup. Animalderived product like the others meat is the best media for the growing of microorganims/bacteria. Somemethods were also done to preserve meat. An experiment has conducted to study the effect of irradiationon pathogenic bacteria which inoculated at goat meat and bowel. Some pathogenic bacteria such asSalmonella agona, Salmonella kentucky and Staphylococcus aureus were inoculated on the goat meat andbowel, respectively. The measured parameter was the amount of colonies which still survive afterirradiation at 0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 kGy. The irradiaton was done at a multipurpose panoramicbatch irradiator (PANBIT) with the dose rate of 2.657 kGy/h. Results showed that Salmonella was moreradioresistant compared to S. aureus. The D10 value of S. agona for goat meat and bowel were 0.31 and0.65 kGy, while D10 value of S. kentucky were 0.68 and 0.79 kGy. On the otherhand, D10 value of S.aureus were 0.58 and 0.64 kGy.140 INDRANINGSIH.Pesticide residues in milk and animal feeds in some areas of Java. Residu pestisida dalam susu segardan pakan dari beberapa daerah di Jawa/Indraningsih; Sani, Y. (Balai Penelitian Veteriner, Bogor(Indonesia)). [Proceedings of the national seminar on animal husbandry and veterinary technology], Bogor(Indonesia) 12-13 Sep 2005/Mathius, I W.; Bahri, S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.;Tiesnamurti, B.; Sendow, I.; Suhardono (eds.) Pusat Penelitian dan Pengembangan Peternakan, Bogor(Indonesia). Bogor: Puslitbangnak, 2005: p. 956-962, 2 ill., 2 tables; 21 ref. 636:338.439/SEM/pMILK; PESTICIDES; RESIDUES; FEEDS; CONTAMINATION; ORGANOCHLORINECOMPOUNDS; PHOSPHATES; JAVA.Analysis of pesticide residues had been undertaken in milk and animal feeds collected from some placesof West Java (Bogor, Lembang and Pangalengan), Central Java (Solo) and East Java (<strong>No</strong>ngkojajar andNgantang). The purposes of this study were to investigate the status of pesticide residues in milk andanimal feeds and a source of contamination in milk. The samples were extracted with organic solvents anddetected by gas chromatography. The results showed that both groups of pesticides (organochlorines/OCsand organophosphates/OPs) were detected in milk of the three provinces. Milk of Central Java has thehighest level of total pesticide residues (13.15 ppb) compares to West Java (11.15 ppb) and East Java(1.06 ppb). The OP residues in milk were higher than OC in Central Java (10.65 ppb vs 2.5 ppb) and WestJava (5.93 ppb vs 5.22 ppb), but not detected in East Java. Similar situation was also noted in animal feedsto which the total pesticide residues were 186.25 ppb (Central Java), 134.57 ppb (West Java) and 54.82ppb (East Java) subsequently. The OP residues in feeds were higher than OC in all provinces as shown inWest Java (129.18 ppb vs 5.39 ppb), Central Java (97.86 ppb vs 88.39 ppb) and East Java (52.72 ppb vs2.1 ppb). The OCs were still higher in animal feeds of Central Java at the level of 88.39 ppb. It appearedthat there was a correlation between contamination of animal feeds and the occurence of pesticide residuesin milk. Pesticide contaminated feed has an important role as source of contamination sources for milk.69
<strong>Vol</strong>. 26, <strong>No</strong>. 1, <strong>2009</strong>Indonesian Agricultural Research Abstracts141 MISGIYARTA.Concentrations of antibiotic residues in fresh milk. Status tingkat residu antibiotik pada sususegar/Misgiyarta; Roswita S.; Munarso, S.J.; Abubakar; Usmiati, S. (Balai Besar Penelitian danPengembangan Pascapanen Pertanian, Bogor (Indonesia)). [Proceedings of the national seminar on animalhusbandry and veterinary technology. Book 1], Bogor (Indonesia) 12-13 Sep 2005/Mathius, I W.; Bahri,S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.; Tiesnamurti, B.; Sendow, I.; Suhardono (eds.) PusatPenelitian dan Pengembangan Peternakan, Bogor (Indonesia). Bogor: Puslitbangnak, 2005: p. 206-214, 6tables; 12 ref. 636:338.439/SEM/pCOW MILK; ANTIBIOTICS; RESIDUES; QUALITY.Milk is an important livestock product commodity as a very good source of nutrition produced by dairycattle at dairy farms centers. Relatively few number of cattle owned, rearing method and inadequatepostharvest handling result in low quality milk. Low quality milk causes farmers having very wea<strong>kb</strong>argaining position to obtain high fresh milk price. The improvement of milk quality is crucial to becarried out, which eventually will increase the income of dairy cattle farmers. Before conducting theefforts on improving the milk quality, it is necessary to find out the initial milk quality. The milkprocessing industries start implementing the requirements concerning the price in accepting fresh milkincluding the present of antibiotic contaminants. Researches to find out the milk quality were conducted atKSU Tandang Sari, Tanjung Sari, Sumedang, and KUD Sarwamukti, Lembang, West Java. Thecontaminants observed were antibiotic residues including penicillin, oxytetracycline, tetracycline, andchlortetracycline. The milk residue concentrations measured were at the level of farmers, collectors, andcooperative bodies. The concentrations of residues were analyzed by using high pressure liquidchromatography (HPLC) method. The antibiotic concentrations in fresh milk from KSU Tandang Sari andKUD Sarwamukti areas varied. The antibiotic residue concentrations in ppm at the farmer levels arepenicillin 0.0023, tetracycline 0.0002, oxytetracycline 0.0002, and chlortetracycline 0.0055. At the level ofcollectors, the antibiotic residue concentrations in ppm are penicillin 0.0008, tetracycline 0.0002,oxytetracycline 0.0002, and chlortetracycline 0.0037. The antibiotic residue concentrations in ppm at thelevel of cooperative body are: penicillin undetected, tetracycline undetected, oxytetracycline undetected,and chlortetracycline 0.02. The Indonesian National Standard (Standar Nasional Indonesia (SNI)) 01-6366-2000 allows a maximum limit for antibiotic in fresh milk amounted (ppm); penicillin 0.1,tetracycline 0.05, oxytetracycline 0.05, and chlortetracycline 0.05. The concentrations of antibioticresidues in fresh milk are still safe as they are still below the maximum antibiotic limit recommended bySNI 01-6366-2000.142 RACHMAWATI, S.ELISA kit (Aflavet) for detecting aflatoxin in agricultural product. Kit ELISA (Aflavet) untuk deteksiaflatoksin pada produk pertanian/Rachmawati, S. (Balai Penelitian Veteriner, Bogor (Indonesia)).[Proceedings of the national seminar on animal husbandry and veterinary technology], Bogor (Indonesia)12-13 Sep 2005/Mathius, I W.; Bahri, S.; Tarmudji; Prasetyo, L.H.; Triwulanningsih, E.; Tiesnamurti, B.;Sendow, I.; Suhardono (eds.) Pusat Penelitian dan Pengembangan Peternakan, Bogor (Indonesia). Bogor:Puslitbangnak, 2005: p. 1105-1110, 2 ill., 1 table; 36 ref. 636:338.439/SEM/pFOODS; FEEDS; GROUNDNUTS; MAIZE; CONTAMINATION; AFLATOXINS; ELISA;CHEMILUMINESCENCE; METHOD; QUALITY CONTROLS.Aflatoxin is a carcinogenic toxic compound which is dangerous for animal and human health. ResearchInstitute for Veterinary Bogor has been developed an ELISA method for AFB1 analysis named Af1avet,which has been validated with an accurate and consistent results compared to standard method ofchromatography. In this paper the use of the ELISA kit was applied for AFB1 analysis in peanut, corn as afeed ingredient and poultry feed. Twenty of peanut samples including peanut butter, 12 corn samples and20 of feed samples were collected from tradisional market, supermarket and poultry shops around Bogor.Samples were grounded, extracted with 60% methanol, centrifuged, and the supernatant were taken for70
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