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Before using the FireStingO2 and its sensors, read carefully theinstructions and user manual for the oxygen meter FireStingO2.In case of problems or damage, disconnect the instrument andmark it to prevent any further use! Consult PyroScience foradvice! There are no serviceable parts inside the device. Pleasenote that opening the housing will void the warranty!The FireStingO2 is not watertight, is sensitive to corrosiveconditions and to changes in temperature causingcondensation. Avoid any condition (e.g. direct sun light) causinga heating of the device above 50°C (122°F) or below 0°C. Avoidany elevated humidity causing condensing conditions.Handle the sensors with care especially after removal of theprotective cap! Prevent mechanical stress to the fragile sensingtip! Avoid strong bending of the fiber cable! Prevent injurieswith needle-type sensors!Calibration and application of the sensors is on the user’sauthority, as well as data acquisition, treatment andpublication!The sensors and the oxygen meter FireStingO2 are not intendedfor medical, diagnostic, therapeutic, or military purposes or anyother safety-critical applications. The sensors must not be usedfor applications in humans and should not be brought in directcontact with foods intended for consumption by humans.The FireStingO2 and sensors should be used in the laboratoryonly by qualified personal following the user instructions andthe safety guidelines of the manual, as well as the appropriatelaws and guidelines for safety in the laboratory!Keep the sensors and the oxygen meter FireStingO2 outside thereach of children!10
3 Introduction to the FireStingO2The FireStingO2 is an optical oxygen meter that is compatible witha broad range of oxygen sensors from PyroScience:microsensors and minisensors (retractable needle-type,fixed needle-type, or bare fiber),robust probes andcontactless sensors (sensor spots, respiration vials, flowthroughcells)Most sensors are available in versions for the full range (0-100%O2) and for the trace range (0-21% O2). The optical detectiontechnology is based on the unique oxygen-sensitive REDFLASHindicators which use red light excitation and lifetime detection inthe near infrared (see Appendix 12.4 for more details).The FireStingO2 is a high precision, compact USB-powered fiberopticoxygen meter with 1, 2 or 4 channels for measurements inthe laboratory. Additionally, one temperature sensor can beconnected for automatic temperature compensation of the oxygenmeasurement. The integrated sensors for atmospheric pressure(mbar) and relative humidity (%RH) of the ambient air enable aprecise and easy sensor calibration, as well as automatic pressurecompensation of the oxygen measurements.The FireStingO2 is operated via a Micro-USB connection to aPC/tablet with a Windows operation system. The included loggingsoftware Pyro Oxygen Logger provides comfortable calibrationand logging functionality.The FireStingO2 is a laboratory instrument, and if used in the field,please protect the FireStingO2 from heating, moisture andcorrosion.11
Page 2 and 3: Document Version 3.07Refers to Pyro
Page 4 and 5: 7.1.6 Calibration Mode: Custom Mode
Page 6 and 7: 1 OverviewThe compact USB-powered f
Page 8 and 9: 2 Safety GuidelinesThe FireStingO2
Page 12 and 13: The FireStingO2 comes with 1, 2, or
Page 14 and 15: 5 Oxygen Sensor TypesFor an overvie
Page 16 and 17: position 0 (retracted position) of
Page 18 and 19: (P) to the oxygen meter FireStingO2
Page 20 and 21: oxygen towards the 3 mm tip has a m
Page 22 and 23: It is important to fix or glue the
Page 24 and 25: 5.7 Connecting the SensorsThe fiber
Page 26 and 27: The bare fiber sensors need to be s
Page 28 and 29: Note that placing the mouse on many
Page 30 and 31: The unit of the x-axis can be chang
Page 32 and 33: Signal too high There might be too
Page 34 and 35: continuous sampling (default settin
Page 36 and 37: In the settings the user has to def
Page 38 and 39: 6.2.2 Advanced SettingsIf Advanced
Page 40 and 41: of the respective oxygen sensor rea
Page 42 and 43: After activation of a temperature s
Page 44 and 45: The activation of the Analog In but
Page 46 and 47: 6.4 Raw Data WindowThe Raw Data Win
Page 48 and 49: 7 Calibration with “Pyro Oxygen L
Page 50 and 51: calibration in air saturated water
Page 52 and 53: If the Sensor code is correct, the
Page 54 and 55: NOTE: If using retractable needle-t
Page 56 and 57: NOTE: If using retractable needle-t
Page 58 and 59: Note that the button Set 0% will be
Page 60 and 61:
(°C). Note that the button Set Air
Page 62 and 63:
And for the 0% calibration the foll
Page 64 and 65:
Consequently, the 0% calibration sh
Page 66 and 67:
7.2.1 Calibration Mode: Factory (1.
Page 68 and 69:
due to the actual weather condition
Page 70 and 71:
Wait for steady state until the sen
Page 72 and 73:
7.3 Advanced adjustmentsThis sectio
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8 Calibration Standards8.1 The Air
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elevation the typical atmospheric p
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8.2 The 0% StandardThe 0% calibrati
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9 Calibration of Contactless Sensor
Page 82 and 83:
one end of the Optical Fiber is con
Page 84 and 85:
10.3 Automatic Temperature Compensa
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11 Analog Output and Auto-ModeThe s
Page 88 and 89:
voltage signals proportional to the
Page 90 and 91:
Here the Auto-Mode Sample Interval
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trigger signal was detected at the
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External Temperature Sensors*Range,
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Pin Name Function Description1 GND
Page 98 and 99:
Auto-Mode with External Power Suppl
Page 100 and 101:
12.2.2 Connector X2 (Analog Output)
Page 102 and 103:
12.4 Measuring PrincipleThe new RED
Page 104 and 105:
12.5 Operating several FireStingO2
Page 106 and 107:
with p100 O2 = 0.2095 ( p atm - p H
Page 108 and 109:
Sal(PSU)Temp0(°C)5 10 15 20 25 30
Page 110:
LED IntensityA 10% E 40%B 15% F 60%
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