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It is important to fix or glue the spot adapter firmly to the outercontainer wall. The position of the spot adapter should not bechanged after calibration of the sensor spot (otherwise a recalibrationmight be needed).The sensor spots offer a versatile field of application for multisamplingmeasurements and online-monitoring of oxygen atgreater scales. This includes the application in e.g. respiration andphotosynthesis chambers, bioreactors, studies of enzyme kinetics,cell biological approaches and industrial process water-monitoring.5.5 Flow-Through CellsThe flow-through cells have an integratedoxygen sensor, allowing onlinemonitoringof the oxygen content in a gasor liquid sample pumped through the cell.The flow-through cells are offered as asmall version (item no. OXFTC) and as a large version (item no.OXFTC2). The attached tubing has an outer/inner diameter of ca.2/1.5 mm for the OXFTC and of ca. 7/4 mm for the OXFTC2.The flow-through cells are equipped with luer-lock connectors onboth tubing ends. A set of luer-lock adapters is included allowingthe direct connection of gas-tight tubings with ca. 1.5-3.0 mminner diameter tothe OXFTC andca. 4.0-5.5 mm tothe OXFTC2.The flow-through cell can be directly connected with the OpticalFiber (item no. SPFIB or SPFIB-BARE) to the FireStingO2.CAUTION: For measurements in liquids, a flow rate of ca. 10-100mL min -1 is recommended for the OXFTC and of ca. 20-500 mLmin -1 for the OXFTC2!22
5.6 Respiration VialsBased on the contactless sensortechnology from Pyro Science, readyassembled respiration vials withintegrated oxygen sensors areavailable with 4ml or 20ml volume(item no. OXVIAL4 and OXVIAL20),respectively. Stripes of the oxygen sensitive REDFLASH indicatorare glued to the inner wall of the respiration vials. Separatelyavailable adapter rings (item no. ADVIAL4 and ADVIAL20) allowan easy fixation of a bare optical fiber (item no. SPFIB-BARE orSPFIB-BARE-CL2), which connects the respiration vial to onechannel of the FireStingO2. The bare-fiber-versions of the opticalfiber allow submersion in a water bath, as their fiber ends do notcontain metallic parts potentially prone to corrosion. The positionof the adapter rings (and the spot fiber) can be fixed by clampingscrews. The elongated shape of the built-in sensor allowsoxygen measurements at different heights within the vial bysimply sliding the adapter rings up and down. Therefore it is e.g.possible to measure the oxygen content both in a liquid and inthe gaseous headspace within the same vial.For high precision respiration rate measurements, the respirationvials would be typically placed within a water bath with a regulatedconstant temperature at 0.1°C precision. If higher temperaturefluctuations are expected, the oxygen measurement should beautomatically temperature compensated by connectinga temperature sensor (item no. TDIP15 or TSUB36) to theFireStingO2 (see chapter 10) and placing it in the water bath.The respiration vials can be used for small-scale respirometry andmetabolic rate measurements of e.g. cell cultures, eggs, larvae,small crustaceans, small fish, water-, plant-, algal-samples etc.23
Page 2 and 3: Document Version 3.07Refers to Pyro
Page 4 and 5: 7.1.6 Calibration Mode: Custom Mode
Page 6 and 7: 1 OverviewThe compact USB-powered f
Page 8 and 9: 2 Safety GuidelinesThe FireStingO2
Page 10 and 11: Before using the FireStingO2 and it
Page 12 and 13: The FireStingO2 comes with 1, 2, or
Page 14 and 15: 5 Oxygen Sensor TypesFor an overvie
Page 16 and 17: position 0 (retracted position) of
Page 18 and 19: (P) to the oxygen meter FireStingO2
Page 20 and 21: oxygen towards the 3 mm tip has a m
Page 24 and 25: 5.7 Connecting the SensorsThe fiber
Page 26 and 27: The bare fiber sensors need to be s
Page 28 and 29: Note that placing the mouse on many
Page 30 and 31: The unit of the x-axis can be chang
Page 32 and 33: Signal too high There might be too
Page 34 and 35: continuous sampling (default settin
Page 36 and 37: In the settings the user has to def
Page 38 and 39: 6.2.2 Advanced SettingsIf Advanced
Page 40 and 41: of the respective oxygen sensor rea
Page 42 and 43: After activation of a temperature s
Page 44 and 45: The activation of the Analog In but
Page 46 and 47: 6.4 Raw Data WindowThe Raw Data Win
Page 48 and 49: 7 Calibration with “Pyro Oxygen L
Page 50 and 51: calibration in air saturated water
Page 52 and 53: If the Sensor code is correct, the
Page 54 and 55: NOTE: If using retractable needle-t
Page 56 and 57: NOTE: If using retractable needle-t
Page 58 and 59: Note that the button Set 0% will be
Page 60 and 61: (°C). Note that the button Set Air
Page 62 and 63: And for the 0% calibration the foll
Page 64 and 65: Consequently, the 0% calibration sh
Page 66 and 67: 7.2.1 Calibration Mode: Factory (1.
Page 68 and 69: due to the actual weather condition
Page 70 and 71: Wait for steady state until the sen
Page 72 and 73:
7.3 Advanced adjustmentsThis sectio
Page 74 and 75:
8 Calibration Standards8.1 The Air
Page 76 and 77:
elevation the typical atmospheric p
Page 78 and 79:
8.2 The 0% StandardThe 0% calibrati
Page 80 and 81:
9 Calibration of Contactless Sensor
Page 82 and 83:
one end of the Optical Fiber is con
Page 84 and 85:
10.3 Automatic Temperature Compensa
Page 86 and 87:
11 Analog Output and Auto-ModeThe s
Page 88 and 89:
voltage signals proportional to the
Page 90 and 91:
Here the Auto-Mode Sample Interval
Page 92 and 93:
trigger signal was detected at the
Page 94 and 95:
External Temperature Sensors*Range,
Page 96 and 97:
Pin Name Function Description1 GND
Page 98 and 99:
Auto-Mode with External Power Suppl
Page 100 and 101:
12.2.2 Connector X2 (Analog Output)
Page 102 and 103:
12.4 Measuring PrincipleThe new RED
Page 104 and 105:
12.5 Operating several FireStingO2
Page 106 and 107:
with p100 O2 = 0.2095 ( p atm - p H
Page 108 and 109:
Sal(PSU)Temp0(°C)5 10 15 20 25 30
Page 110:
LED IntensityA 10% E 40%B 15% F 60%
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