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oxygen towards the 3 mm tip has a measurable effect on theresponse time. For optimum operation conditions it is thereforerecommended to utilize these robust probes in permanently stirredliquids (this diffusion limitation is negligible in gaseous samples;see also the respective sensor specifications).Ensure that the complete sensing surface of the Robust OxygenProbe is covered completely by the sample and preventmechanical stress (e.g. scratching) to the sensing surface!5.4 Sensor SpotsPyroScience offers arange of contactlessoxygen sensors,comprising sensorspots, respirationvials and flowthroughcells. Thesensor spots arecoated with theREDFLASH indicatorwith PET foil or glassas carrier material.The sensor spots allow oxygen measurements in closed vessels incombination with the FireStingO2 equipped with Optical Fibers(e.g. item no. SPFIB or SPFIB-CL2). The sensor spots havestandard diameters of 5 mm (item no. OXSP5) and can beoptionally obtained with an optical isolation (black coating) on thesensing surface (e.g. item no. OXSP5OI, OXSG8OI). Opticalisolation is recommended if strong illuminationmight disturb the measurement or if no light shallbe introduced into the sample.20
The sensor spots have a rough greensensing surface. Their backside is greensmooth and shiny, and adheres on theadhesive side of the packaging (on whichthe sensor code is printed on the outside).The sensor spots can be glued with their backside on transparent,clean and dry inner container walls (plastic or glass, wall thickness0-6 mm) using an appropriate adhesive, like e.g. transparentsilicone based on acetic acid (item no. SPGLUE). After the glue hasdried, the gas or liquid sample has to be filled into the container sothat the rough green sensing surface of the spot is completelycovered and in contact with the sample.For containers with wall thicknesses of 0-2 mm, theBasic Spot Adapter (a 10x10x10 mm plastic cube,item no. SPADBAS) needs to be fixed tightly orglued to the outer container wall at the spotposition. Then the sensor signal can be read outusing the Optical Fiber (item no. SPFIB or thebare-fiber-version SPFIB-BARE) connecting thebasic spot adapter with the FireStingO2.For measurements through containers with wall thicknesses of 2-6 mm, the metallic Lens Spot Adapter (item no.SPADLNS) with an integrated collimating lens hasto be used instead. It comes together with a flexiblere-adjustable cable binder for quick positioning of spot adapterse.g. on laboratory flasks with a maximal diameter of ~10 cm.Please note, that the Lens Spot Adapter doesnot work when submersed in water, because theintegrated lens is working properly only in thegas phase. The spot adapter is then connectedwith the Optical Fiber (item no. SPFIB, SPFIB-BARE) to one channel of the FireStingO2.21
Page 2 and 3: Document Version 3.07Refers to Pyro
Page 4 and 5: 7.1.6 Calibration Mode: Custom Mode
Page 6 and 7: 1 OverviewThe compact USB-powered f
Page 8 and 9: 2 Safety GuidelinesThe FireStingO2
Page 10 and 11: Before using the FireStingO2 and it
Page 12 and 13: The FireStingO2 comes with 1, 2, or
Page 14 and 15: 5 Oxygen Sensor TypesFor an overvie
Page 16 and 17: position 0 (retracted position) of
Page 18 and 19: (P) to the oxygen meter FireStingO2
Page 22 and 23: It is important to fix or glue the
Page 24 and 25: 5.7 Connecting the SensorsThe fiber
Page 26 and 27: The bare fiber sensors need to be s
Page 28 and 29: Note that placing the mouse on many
Page 30 and 31: The unit of the x-axis can be chang
Page 32 and 33: Signal too high There might be too
Page 34 and 35: continuous sampling (default settin
Page 36 and 37: In the settings the user has to def
Page 38 and 39: 6.2.2 Advanced SettingsIf Advanced
Page 40 and 41: of the respective oxygen sensor rea
Page 42 and 43: After activation of a temperature s
Page 44 and 45: The activation of the Analog In but
Page 46 and 47: 6.4 Raw Data WindowThe Raw Data Win
Page 48 and 49: 7 Calibration with “Pyro Oxygen L
Page 50 and 51: calibration in air saturated water
Page 52 and 53: If the Sensor code is correct, the
Page 54 and 55: NOTE: If using retractable needle-t
Page 56 and 57: NOTE: If using retractable needle-t
Page 58 and 59: Note that the button Set 0% will be
Page 60 and 61: (°C). Note that the button Set Air
Page 62 and 63: And for the 0% calibration the foll
Page 64 and 65: Consequently, the 0% calibration sh
Page 66 and 67: 7.2.1 Calibration Mode: Factory (1.
Page 68 and 69: due to the actual weather condition
Page 70 and 71:
Wait for steady state until the sen
Page 72 and 73:
7.3 Advanced adjustmentsThis sectio
Page 74 and 75:
8 Calibration Standards8.1 The Air
Page 76 and 77:
elevation the typical atmospheric p
Page 78 and 79:
8.2 The 0% StandardThe 0% calibrati
Page 80 and 81:
9 Calibration of Contactless Sensor
Page 82 and 83:
one end of the Optical Fiber is con
Page 84 and 85:
10.3 Automatic Temperature Compensa
Page 86 and 87:
11 Analog Output and Auto-ModeThe s
Page 88 and 89:
voltage signals proportional to the
Page 90 and 91:
Here the Auto-Mode Sample Interval
Page 92 and 93:
trigger signal was detected at the
Page 94 and 95:
External Temperature Sensors*Range,
Page 96 and 97:
Pin Name Function Description1 GND
Page 98 and 99:
Auto-Mode with External Power Suppl
Page 100 and 101:
12.2.2 Connector X2 (Analog Output)
Page 102 and 103:
12.4 Measuring PrincipleThe new RED
Page 104 and 105:
12.5 Operating several FireStingO2
Page 106 and 107:
with p100 O2 = 0.2095 ( p atm - p H
Page 108 and 109:
Sal(PSU)Temp0(°C)5 10 15 20 25 30
Page 110:
LED IntensityA 10% E 40%B 15% F 60%
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