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5.7 Connecting the SensorsThe fiber-optic oxygen sensors, including needle-type and barefiber micro- and minisensors, robust probes, as well as opticalfibers needed for contactless sensors (sensor spots, flow-throughcells, respiration vials) are connected to the ST-connectors of theFireStingO2 (1 to 4) with a male fiber plug.First, removethe black capsfrom the plug ofthe sensor/fiber.Remove the red caps from the receptacles at the FireStingO2 (thered caps should be put on again if the FireStingO2 is not in useanymore to protect the optics).Then insert the male fiber plug (M) of the sensor cable into the STreceptacle(female fiber connector) of the FireStingO2 and turn thebayonet coupling gently clockwise until the plug is locked firmly.24
5.8 Cleaning and Maintenance of the SensorsAll oxygen sensors can be sterilized with ethylene oxide (EtO) andcan be cleaned with peroxide (3% H2O2), soap solution or ethanol.They can be applied in gas phases, aqueous solutions, ethanol,methanol and isopropanol. Other organic solvents and gaseouschlorine (Cl2) induce interferences with the sensor reading. Nocross-sensitivity is found for pH 1-14, CO2, CH4, H2S and any ionicspecies.A signal drift of the sensor can indicate photobleaching of theoxygen-sensitive REDFLASH indicator, depending on the ambientlight intensity, as well as the intensity of the excitation light andthe sample frequency. This can necessitate a recalibration of thesensor and eventually also a readjustment of the Sensor Settings(LED intensity; see also chapter 6.2.2). In case of sensor spots, thiscould require a re-positioning of the optical fiber on the sensorspot and a subsequent new calibration. If the signal intensity isgetting too low, as indicated by the horizontal indicator bar in thePyro Oxygen Logger software and by the respective warning (seechapter 6), the sensor needs to be replaced.A reasonable normal range oxygen sensor shows signal intensitieswell above 20 (typically 50-500).After finalization of the measurements, the sensor tip of theneedle-type and bare fiber oxygen sensors, as well as the robustprobes should be rinsed carefully with demineralized water.Especially after application in seawater, it is recommended toclean the sensor thoroughly with demineralized water to preventsalt crystallization in the needle which can cause breaking of thesensor tip.In case of retractable sensors, retract the sensor tip into the needleafter drying. For all needle-type sensors, put on the protective caponto the needle to protect the sensor tip and to avoid injuries.25
Page 2 and 3: Document Version 3.07Refers to Pyro
Page 4 and 5: 7.1.6 Calibration Mode: Custom Mode
Page 6 and 7: 1 OverviewThe compact USB-powered f
Page 8 and 9: 2 Safety GuidelinesThe FireStingO2
Page 10 and 11: Before using the FireStingO2 and it
Page 12 and 13: The FireStingO2 comes with 1, 2, or
Page 14 and 15: 5 Oxygen Sensor TypesFor an overvie
Page 16 and 17: position 0 (retracted position) of
Page 18 and 19: (P) to the oxygen meter FireStingO2
Page 20 and 21: oxygen towards the 3 mm tip has a m
Page 22 and 23: It is important to fix or glue the
Page 26 and 27: The bare fiber sensors need to be s
Page 28 and 29: Note that placing the mouse on many
Page 30 and 31: The unit of the x-axis can be chang
Page 32 and 33: Signal too high There might be too
Page 34 and 35: continuous sampling (default settin
Page 36 and 37: In the settings the user has to def
Page 38 and 39: 6.2.2 Advanced SettingsIf Advanced
Page 40 and 41: of the respective oxygen sensor rea
Page 42 and 43: After activation of a temperature s
Page 44 and 45: The activation of the Analog In but
Page 46 and 47: 6.4 Raw Data WindowThe Raw Data Win
Page 48 and 49: 7 Calibration with “Pyro Oxygen L
Page 50 and 51: calibration in air saturated water
Page 52 and 53: If the Sensor code is correct, the
Page 54 and 55: NOTE: If using retractable needle-t
Page 56 and 57: NOTE: If using retractable needle-t
Page 58 and 59: Note that the button Set 0% will be
Page 60 and 61: (°C). Note that the button Set Air
Page 62 and 63: And for the 0% calibration the foll
Page 64 and 65: Consequently, the 0% calibration sh
Page 66 and 67: 7.2.1 Calibration Mode: Factory (1.
Page 68 and 69: due to the actual weather condition
Page 70 and 71: Wait for steady state until the sen
Page 72 and 73: 7.3 Advanced adjustmentsThis sectio
Page 74 and 75:
8 Calibration Standards8.1 The Air
Page 76 and 77:
elevation the typical atmospheric p
Page 78 and 79:
8.2 The 0% StandardThe 0% calibrati
Page 80 and 81:
9 Calibration of Contactless Sensor
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one end of the Optical Fiber is con
Page 84 and 85:
10.3 Automatic Temperature Compensa
Page 86 and 87:
11 Analog Output and Auto-ModeThe s
Page 88 and 89:
voltage signals proportional to the
Page 90 and 91:
Here the Auto-Mode Sample Interval
Page 92 and 93:
trigger signal was detected at the
Page 94 and 95:
External Temperature Sensors*Range,
Page 96 and 97:
Pin Name Function Description1 GND
Page 98 and 99:
Auto-Mode with External Power Suppl
Page 100 and 101:
12.2.2 Connector X2 (Analog Output)
Page 102 and 103:
12.4 Measuring PrincipleThe new RED
Page 104 and 105:
12.5 Operating several FireStingO2
Page 106 and 107:
with p100 O2 = 0.2095 ( p atm - p H
Page 108 and 109:
Sal(PSU)Temp0(°C)5 10 15 20 25 30
Page 110:
LED IntensityA 10% E 40%B 15% F 60%
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