The qpcR Package - NexTag Supports Open Source Initiatives

10 efficiencyValueA list with the following components:effresVarAICcAICRsqcpD1cpD2cpEcpRfluoinitcfthe PCR efficiency.the residual variance.the bias-corrected Akaike Information Criterion.the Akaike Information Criterion.the R-square value.the first derivative maximum (point of inflection in ’l4’ or ’b4’ models, can beused for defining the threshold cycle).the second derivative maximum (turning point of cpD1, more often used fordefining the threshold cycle).the PCR cycle with the highest efficiency, if "maxE" method is chosen.the PCR cycle within the exponential region calculated as under ’Details’, if"expR" method is chosen.the raw fluorescence value at the point defined by type.the initial template fluorescence, i.e. F(0), calculated as under ’Details’.the conversion factor between raw fluorescence and template amount, if the latteris defined.NoteThree parameter models (’b3’ or ’l3’) do not work very well in calculating the PCR efficiency. Itis advisable not to take too many cycles of the plateau phase prior to fitting the model as this has astrong effect on the validity of the efficiency estimates.Author(s)Andrej-Nikolai Spiess & Christian RitzReferencesWeihong Liu and David A. Saint (2002) Validation of a quantitative method for real time PCRkinetics, BBRC, 294, 347 - 353.Examples## Fitting initial modelm1